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Database: UniProt
Entry: ACR2_CAEEL
LinkDB: ACR2_CAEEL
Original site: ACR2_CAEEL 
ID   ACR2_CAEEL              Reviewed;         575 AA.
AC   P48182;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   18-SEP-2019, entry version 146.
DE   RecName: Full=Acetylcholine receptor subunit beta-type acr-2;
DE   Flags: Precursor;
GN   Name=acr-2; ORFNames=K11G12.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH NICOTINIC
RP   ACETYLCHOLINE RECEPTOR.
RC   STRAIN=Bristol N2;
RX   PubMed=8581398;
RA   Squire M.D., Tornoe C.A., Baylis H.A., Fleming J.T., Barnard E.A.,
RA   Sattelle D.B.;
RT   "Molecular cloning and functional co-expression of a Caenorhabditis
RT   elegans nicotinic acetylcholine receptor subunit (acr-2).";
RL   Recept. Channels 3:107-115(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
RN   [3]
RP   FUNCTION, INTERACTION WITH NICOTINIC ACETYLCHOLINE RECEPTOR, TISSUE
RP   SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND
RP   MUTAGENESIS OF VAL-309.
RX   PubMed=20027209; DOI=10.1371/journal.pbio.1000265;
RA   Jospin M., Qi Y.B., Stawicki T.M., Boulin T., Schuske K.R.,
RA   Horvitz H.R., Bessereau J.L., Jorgensen E.M., Jin Y.;
RT   "A neuronal acetylcholine receptor regulates the balance of muscle
RT   excitation and inhibition in Caenorhabditis elegans.";
RL   PLoS Biol. 7:E1000265-E1000265(2009).
RN   [4]
RP   FUNCTION, AND MUTAGENESIS OF VAL-309.
RX   PubMed=23658528; DOI=10.1371/journal.pgen.1003472;
RA   Stawicki T.M., Takayanagi-Kiya S., Zhou K., Jin Y.;
RT   "Neuropeptides function in a homeostatic manner to modulate
RT   excitation-inhibition imbalance in C. elegans.";
RL   PLoS Genet. 9:E1003472-E1003472(2013).
RN   [5]
RP   FUNCTION.
RX   PubMed=27782882; DOI=10.7554/elife.21734;
RA   Takayanagi-Kiya S., Zhou K., Jin Y.;
RT   "Release-dependent feedback inhibition by a presynaptically localized
RT   ligand-gated anion channel.";
RL   Elife 5:21734-21749(2016).
CC   -!- FUNCTION: Non-alpha subunit of nicotinic acetylcholine receptor
CC       (nAChR) (PubMed:8581398, PubMed:20027209). Acts in cholinergic
CC       motoneurons to regulate presynaptic neurotransmitter release,
CC       thereby ensuring normal level of excitation of cholinergic
CC       motoneurons during locomotion (PubMed:20027209, PubMed:23658528,
CC       PubMed:27782882). {ECO:0000269|PubMed:20027209,
CC       ECO:0000269|PubMed:23658528, ECO:0000269|PubMed:8581398}.
CC   -!- SUBUNIT: Component of nicotinic acetylcholine receptor. In
CC       cholinergic motoneurons, composed of 2 non-alpha subunits acr-2
CC       and acr-3, and 3 alpha subunits unc-38, unc-63 and acr-12.
CC       {ECO:0000269|PubMed:20027209, ECO:0000269|PubMed:8581398}.
CC   -!- SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell
CC       membrane {ECO:0000250|UniProtKB:Q23022}; Multi-pass membrane
CC       protein {ECO:0000255}. Cell membrane
CC       {ECO:0000250|UniProtKB:Q23022}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Specifically expressed in cholinergic ventral
CC       cord motoneurons of the VA, VB, DA and DB classes but not AS and
CC       VC classes. Expressed in PVQ and DVC neurons in the tail.
CC       {ECO:0000269|PubMed:20027209}.
CC   -!- DEVELOPMENTAL STAGE: Expressed from L1 larval stage through
CC       adults. {ECO:0000269|PubMed:20027209}.
CC   -!- DISRUPTION PHENOTYPE: Locomotion is slower. Moderately resistant
CC       to paralysis induced by acetylcholine esterase inhibitor aldicarb
CC       but sensitivity to acetylcholine agonist levamisole is normal.
CC       Reduced both cholinergic and GABAergic motoneuron activities
CC       characterized by a reduction in miniature postsynaptic currents in
CC       muscles. {ECO:0000269|PubMed:20027209}.
CC   -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9)
CC       family. Acetylcholine receptor (TC 1.A.9.1) subfamily.
CC       {ECO:0000305}.
DR   EMBL; X86403; CAA60157.1; -; mRNA.
DR   EMBL; FO081325; CCD70794.1; -; Genomic_DNA.
DR   PIR; S57648; S57648.
DR   RefSeq; NP_509128.1; NM_076727.5.
DR   SMR; P48182; -.
DR   STRING; 6239.K11G12.2; -.
DR   TCDB; 1.A.9.1.13; the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.
DR   TCDB; 1.A.9.1.2; the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.
DR   PaxDb; P48182; -.
DR   PRIDE; P48182; -.
DR   EnsemblMetazoa; K11G12.2.1; K11G12.2.1; WBGene00000042.
DR   GeneID; 180936; -.
DR   KEGG; cel:CELE_K11G12.2; -.
DR   UCSC; K11G12.2; c. elegans.
DR   CTD; 180936; -.
DR   WormBase; K11G12.2; CE07380; WBGene00000042; acr-2.
DR   eggNOG; KOG3645; Eukaryota.
DR   eggNOG; ENOG410XQGR; LUCA.
DR   HOGENOM; HOG000006756; -.
DR   InParanoid; P48182; -.
DR   KO; K05312; -.
DR   OMA; PHVFDFV; -.
DR   OrthoDB; 381858at2759; -.
DR   PhylomeDB; P48182; -.
DR   Reactome; R-CEL-629587; Highly sodium permeable acetylcholine nicotinic receptors.
DR   Reactome; R-CEL-629594; Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
DR   Reactome; R-CEL-629597; Highly calcium permeable nicotinic acetylcholine receptors.
DR   Reactome; R-CEL-6798695; Neutrophil degranulation.
DR   PRO; PR:P48182; -.
DR   Proteomes; UP000001940; Chromosome X.
DR   Bgee; WBGene00000042; Expressed in 4 organ(s), highest expression level in multi-cellular organism.
DR   GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0043005; C:neuron projection; IDA:WormBase.
DR   GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045202; C:synapse; IBA:GO_Central.
DR   GO; GO:0022848; F:acetylcholine-gated cation-selective channel activity; IDA:WormBase.
DR   GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IDA:WormBase.
DR   GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR   GO; GO:0098703; P:calcium ion import across plasma membrane; IDA:WormBase.
DR   GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR   GO; GO:0098662; P:inorganic cation transmembrane transport; IDA:WormBase.
DR   GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR   GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR   GO; GO:0007274; P:neuromuscular synaptic transmission; IMP:UniProtKB.
DR   GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR   GO; GO:0040012; P:regulation of locomotion; IMP:UniProtKB.
DR   GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR   GO; GO:0006937; P:regulation of muscle contraction; IMP:UniProtKB.
DR   GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR   Gene3D; 2.70.170.10; -; 1.
DR   InterPro; IPR006202; Neur_chan_lig-bd.
DR   InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR   InterPro; IPR006201; Neur_channel.
DR   InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR   InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR   InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR   InterPro; IPR002394; Nicotinic_acetylcholine_rcpt.
DR   PANTHER; PTHR18945; PTHR18945; 1.
DR   Pfam; PF02931; Neur_chan_LBD; 1.
DR   Pfam; PF02932; Neur_chan_memb; 1.
DR   PRINTS; PR00254; NICOTINICR.
DR   PRINTS; PR00252; NRIONCHANNEL.
DR   SUPFAM; SSF63712; SSF63712; 1.
DR   SUPFAM; SSF90112; SSF90112; 1.
DR   TIGRFAMs; TIGR00860; LIC; 1.
DR   PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE   1: Evidence at protein level;
KW   Cell junction; Cell membrane; Complete proteome; Disulfide bond;
KW   Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel;
KW   Membrane; Postsynaptic cell membrane; Receptor; Reference proteome;
KW   Signal; Synapse; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL        1     20       {ECO:0000255}.
FT   CHAIN        21    575       Acetylcholine receptor subunit beta-type
FT                                acr-2.
FT                                /FTId=PRO_0000000398.
FT   TOPO_DOM     21    270       Extracellular. {ECO:0000255}.
FT   TRANSMEM    271    291       Helical. {ECO:0000255}.
FT   TRANSMEM    299    319       Helical. {ECO:0000255}.
FT   TRANSMEM    331    351       Helical. {ECO:0000255}.
FT   TOPO_DOM    352    527       Cytoplasmic. {ECO:0000255}.
FT   TRANSMEM    528    548       Helical. {ECO:0000255}.
FT   CARBOHYD     41     41       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD     42     42       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD     80     80       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    131    131       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   DISULFID    185    199       {ECO:0000250}.
FT   MUTAGEN     309    309       V->M: In n2420; constitutively active.
FT                                Spontaneous convulsions and shrinking
FT                                behavior in response to touch.
FT                                Convulsions are suppressed in an unc-74,
FT                                unc-50, unc-38 or unc-63 mutant
FT                                background. Enhanced neurotransmitter
FT                                release by cholinergic motoneurons and
FT                                reduced neurotransmitter release by
FT                                GABAergic motoneurons. Increases
FT                                neuropeptide flp-18 expression in
FT                                cholinergic B-type motor neurons.
FT                                Increases sensitivity to paralysis
FT                                induced by acetylcholine esterase
FT                                inhibitor aldicarb or acetylcholine
FT                                agonist levamisole in L3-L4 larvae and
FT                                adults. {ECO:0000269|PubMed:20027209,
FT                                ECO:0000269|PubMed:23658528}.
SQ   SEQUENCE   575 AA;  65595 MW;  7EB24296100EEB05 CRC64;
     MKKTVKILLI LITVFLKVHC NGGHDDEAAD FLSHTNIDDP NNSSDPNKNS DQGDTMGEDE
     DRLVIDLFRE YNFLIRPVKN VSSPPVVVDF GVAMILLINV DEKNQILQTN VWLTMKWNDF
     QLAWNPAEYG NISNLHVPSD RVWLPDIVLF NNADGNYEVS FKSNVFVDHH GDVTWVPPAM
     FKSSCRIDVE WFPFDEQCCT LVFGSWTYNS EEVRLHWYNN IQAVQLHDYS YSGIWDVIDV
     PGQLVHKPDL KENKMVFNVV IRRKTLFYTV ILIIPTVLMA FLSVMAFYLP VDSGEKVSLT
     ISLLLALVVF LLLVSKILPP TSNIPLMGKY LLLAFVLNIT AVVGTVVIVN IYFRSALSHK
     MPTWVRKVFL EFLPHLLVMK RPERIPIFNG YFVEEYCASE IFDASLVMPS MTATMLPFLQ
     VTTNLKAASS TSSGQSSEHH ENCSKWKKRL SIRMSKRRAP RARLDDDSED IIDDTNGNHV
     DSLQEKISKE MKTTVEAIAY IAEHMKREMS LKKMRDDWKY VAMVLDRLIL LIFFGVTLGG
     TLGIICSAPH VFDFVDQEAI ISKLNAKYLP SDMYS
//
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