ID ALST_BACSU Reviewed; 465 AA.
AC Q45068; P40743;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 132.
DE RecName: Full=Amino-acid carrier protein AlsT;
GN Name=alsT; OrderedLocusNames=BSU18120;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969507; DOI=10.1099/13500872-142-11-3097;
RA Rose M., Entian K.-D.;
RT "New genes in the 170 degrees region of the Bacillus subtilis genome encode
RT DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid
RT transporter.";
RL Microbiology 142:3097-3101(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-465.
RC STRAIN=CK-2;
RX PubMed=7710280; DOI=10.1007/bf00882768;
RA Lindahl V., Aa K., Tronsmo A.;
RT "Nucleotide sequence of an endo-beta-1,4-glucanase gene from Bacillus
RT subtilis CK-2.";
RL Antonie Van Leeuwenhoek 66:327-332(1994).
RN [4]
RP REGULATION BY TNRA.
RX PubMed=12823818; DOI=10.1046/j.1365-2958.2003.03567.x;
RA Yoshida K., Yamaguchi H., Kinehara M., Ohki Y.-H., Nakaura Y., Fujita Y.;
RT "Identification of additional TnrA-regulated genes of Bacillus subtilis
RT associated with a TnrA box.";
RL Mol. Microbiol. 49:157-165(2003).
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Negatively regulated by TnrA under nitrogen-limited
CC conditions.
CC -!- SIMILARITY: Belongs to the alanine or glycine:cation symporter (AGCS)
CC (TC 2.A.25) family. {ECO:0000305}.
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DR EMBL; Z73234; CAA97609.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13695.1; -; Genomic_DNA.
DR EMBL; X67044; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A69585; A69585.
DR RefSeq; NP_389694.1; NC_000964.3.
DR RefSeq; WP_003231542.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; Q45068; -.
DR SMR; Q45068; -.
DR STRING; 224308.BSU18120; -.
DR TCDB; 2.A.25.1.5; the alanine or glycine:cation symporter (agcs) family.
DR PaxDb; 224308-BSU18120; -.
DR EnsemblBacteria; CAB13695; CAB13695; BSU_18120.
DR GeneID; 938191; -.
DR KEGG; bsu:BSU18120; -.
DR PATRIC; fig|224308.179.peg.1976; -.
DR eggNOG; COG1115; Bacteria.
DR InParanoid; Q45068; -.
DR OrthoDB; 9804874at2; -.
DR PhylomeDB; Q45068; -.
DR BioCyc; BSUB:BSU18120-MONOMER; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005283; F:amino acid:sodium symporter activity; IEA:InterPro.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR001463; Na/Ala_symport.
DR NCBIfam; TIGR00835; agcS; 1.
DR PANTHER; PTHR30330; AGSS FAMILY TRANSPORTER, SODIUM-ALANINE; 1.
DR PANTHER; PTHR30330:SF1; AMINO-ACID CARRIER PROTEIN ALST; 1.
DR Pfam; PF01235; Na_Ala_symp; 1.
DR PRINTS; PR00175; NAALASMPORT.
DR PROSITE; PS00873; NA_ALANINE_SYMP; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..465
FT /note="Amino-acid carrier protein AlsT"
FT /id="PRO_0000161568"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 298..300
FT /note="VFF -> KLI (in Ref. 3; X67044)"
FT /evidence="ECO:0000305"
FT CONFLICT 360..361
FT /note="YY -> ID (in Ref. 3; X67044)"
FT /evidence="ECO:0000305"
FT CONFLICT 419
FT /note="A -> T (in Ref. 3; X67044)"
FT /evidence="ECO:0000305"
FT CONFLICT 465
FT /note="A -> V (in Ref. 3; X67044)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 465 AA; 50300 MW; 636B6D843BC6F604 CRC64;
MESFFNSLIN IPSDFIWKYL FYILIGLGLF FTIRFGFIQF RYFIEMFRIV GEKPEGNKGV
SSMQAFFISA ASRVGTGNLT GVALAIATGG PGAVFWMWVV AAVGMASSFV ESTLAQLYKV
RDGEDFRGGP AYYIQKGLGA RWLGIVFAIL ITVSFGLIFN AVQTNTIAGA LDGAFHVNKI
VVAIVLAVLT AFIIFGGLKR VVAVSQLIVP VMAGIYILIA LFVVITNITA FPGVIATIVK
NALGFEQVVG GGIGGIIVIG AQRGLFSNEA GMGSAPNAAA TAHVSHPAKQ GFIQTLGVFF
DTFIICTSTA FIILLYSVTP KGDGIQVTQA ALNHHIGGWA PTFIAVAMFL FAFSSVVGNY
YYGETNIEFI KTSKTWLNIY RIAVIAMVVY GSLSGFQIVW DMADLFMGIM ALINLIVIAL
LSNVAYKVYK DYAKQRKQGL DPVFKAKNIP GLKNAETWED EKQEA
//
