ID ANC2_CAEEL Reviewed; 731 AA.
AC P34514; P34513;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 3.
DT 27-MAR-2024, entry version 168.
DE RecName: Full=Anaphase-promoting complex subunit 2 {ECO:0000312|WormBase:K06H7.6};
DE Short=APC2;
DE AltName: Full=Cyclosome subunit 2;
GN Name=apc-2 {ECO:0000312|WormBase:K06H7.6};
GN ORFNames=K06H7.6/K06H7.5 {ECO:0000312|WormBase:K06H7.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11861581; DOI=10.1093/genetics/160.2.805;
RA Davis E.S., Wille L., Chestnut B.A., Sadler P.L., Shakes D.C., Golden A.;
RT "Multiple subunits of the Caenorhabditis elegans anaphase-promoting complex
RT are required for chromosome segregation during meiosis I.";
RL Genetics 160:805-813(2002).
CC -!- FUNCTION: Probable component of the anaphase promoting
CC complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that
CC controls progression through mitosis and the G1 phase of the cell
CC cycle. The APC/C complex acts by mediating ubiquitination and
CC subsequent degradation of target proteins. Developmental role in early
CC embryogenesis and the metaphase to anaphase transition in meiosis and
CC mitosis. {ECO:0000269|PubMed:11861581}.
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: The APC/C is probably composed of at least 12 subunits: apc-2,
CC apc-10, apc-11, cdc-26, emb-1, emb-27, emb-30, mat-1, mat-2, mat-3,
CC such-1 and gfi-3. {ECO:0000305}.
CC -!- INTERACTION:
CC P34514; Q20052: apc-11; NbExp=8; IntAct=EBI-321211, EBI-314913;
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in defective
CC metaphase to anaphase transition (Mat phenotype) and embryos that
CC arrest at the one-cell stage. {ECO:0000269|PubMed:11861581}.
CC -!- SIMILARITY: Belongs to the cullin family. {ECO:0000255|PROSITE-
CC ProRule:PRU00330}.
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DR EMBL; BX284603; CCD64457.1; -; Genomic_DNA.
DR PIR; S44845; S44845.
DR PIR; S44846; S44846.
DR RefSeq; NP_498762.2; NM_066361.5.
DR AlphaFoldDB; P34514; -.
DR SMR; P34514; -.
DR BioGRID; 41345; 10.
DR ComplexPortal; CPX-3382; Anaphase-promoting complex.
DR IntAct; P34514; 4.
DR STRING; 6239.K06H7.6.1; -.
DR iPTMnet; P34514; -.
DR EPD; P34514; -.
DR PaxDb; 6239-K06H7-6; -.
DR PeptideAtlas; P34514; -.
DR EnsemblMetazoa; K06H7.6.1; K06H7.6.1; WBGene00000143.
DR GeneID; 176139; -.
DR KEGG; cel:CELE_K06H7.6; -.
DR UCSC; K06H7.6; c. elegans.
DR AGR; WB:WBGene00000143; -.
DR WormBase; K06H7.6; CE41679; WBGene00000143; apc-2.
DR eggNOG; KOG2165; Eukaryota.
DR GeneTree; ENSGT00390000016127; -.
DR HOGENOM; CLU_380467_0_0_1; -.
DR InParanoid; P34514; -.
DR OMA; IFRMFAS; -.
DR OrthoDB; 2786196at2759; -.
DR PhylomeDB; P34514; -.
DR Reactome; R-CEL-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; P34514; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:P34514; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00000143; Expressed in germ line (C elegans) and 4 other cell types or tissues.
DR GO; GO:0005680; C:anaphase-promoting complex; IBA:GO_Central.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IEA:InterPro.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; NAS:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007091; P:metaphase/anaphase transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0070979; P:protein K11-linked ubiquitination; IBA:GO_Central.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; NAS:ComplexPortal.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; NAS:ComplexPortal.
DR Gene3D; 1.20.1310.10; Cullin Repeats; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR044554; APC2-like.
DR InterPro; IPR014786; APC2_C.
DR InterPro; IPR016158; Cullin_homology.
DR InterPro; IPR036317; Cullin_homology_sf.
DR InterPro; IPR001373; Cullin_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45957; ANAPHASE-PROMOTING COMPLEX SUBUNIT 2; 1.
DR PANTHER; PTHR45957:SF1; ANAPHASE-PROMOTING COMPLEX SUBUNIT 2; 1.
DR Pfam; PF00888; Cullin; 1.
DR SMART; SM01013; APC2; 1.
DR SMART; SM00182; CULLIN; 1.
DR SUPFAM; SSF75632; Cullin homology domain; 1.
DR PROSITE; PS50069; CULLIN_2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Developmental protein; Meiosis; Mitosis;
KW Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..731
FT /note="Anaphase-promoting complex subunit 2"
FT /evidence="ECO:0000305"
FT /id="PRO_0000119813"
SQ SEQUENCE 731 AA; 84512 MW; BFA67A3C39AC2BB4 CRC64;
MSIFDRMTTS KEAAMKYRQF CVSKKKEVWD ALIYHAKYAK NIEKVYDMTT VLQRVQQECL
MFALEKGVGQ PDIRDAAELI FPLAMQKVVI KVLRPYFMSH FTNYRLAMYL PHRKIDHGID
LNQELWTPEK KEICGQYVQD FNPFVNELKT GILNANRHMN AALSVYIREL AATLVMHEKA
AFDNELRCSM FQEIMESLRI WSDQVTMQDA FHLISDTIYK DVFQMLSKNA YEMIAVDFPV
KFRSLITMKY CLLRTNNHGR VDFTNYLIDQ VNTKLLVASV DTKDILKAYA ACVESLREMD
NSCVVMHKVC GVIREYLKRR PDTVQQIISY ITSNKKNELE KDMSLQSKTV RSAMMDEEEL
KGVNDDFLPE NMETLGWENW MPNPTDATVG DGAPGHQGVD VFNMLVSVYG SKELFVKEYR
NLLAERLSSS DNKDPEFEKR YLDLLKLRFQ YSELQHCEVM LRDVIHSLDI DKKFEDMSER
SAGNYDVPII PISACIISSH YWPKLETEKT EALMPQPLQA AMDVYQETYL DVKRDRKLEW
LRSVGCVEVS INIDGVEVDR TIPNMYALLL FLFLEKETWT TAEVVEKMGM SVVVTRKRLE
WLVKQGFICM NPIISSDTWT LTRNPSGISI VRPGTPDLED DEDVEPEENS DMVDALEQYW
GYTRNFIANH APNGEVKAER MHRVYRMFGS PTSAGPTLDH VSAFLQRKVA LGLLTCINGS
YRIIQEKKDG E
//
