ID AP4S_ARATH Reviewed; 143 AA.
AC O82201;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 24-JAN-2024, entry version 132.
DE RecName: Full=AP-4 complex subunit sigma;
DE AltName: Full=AP-4 adaptor complex subunit sigma;
DE AltName: Full=Adaptor-related protein complex 4 subunit sigma;
DE AltName: Full=Sigma subunit of AP-4;
DE AltName: Full=Sigma4-adaptin;
GN OrderedLocusNames=At2g19790; ORFNames=F6F22.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND REVIEW.
RX PubMed=11598180; DOI=10.1091/mbc.12.10.2907;
RA Boehm M., Bonifacino J.S.;
RT "Adaptins: the final recount.";
RL Mol. Biol. Cell 12:2907-2920(2001).
RN [6]
RP INTERACTION WITH EHD2.
RX PubMed=19936242; DOI=10.1371/journal.pone.0007973;
RA Bar M., Sharfman M., Schuster S., Avni A.;
RT "The coiled-coil domain of EHD2 mediates inhibition of LeEix2 endocytosis
RT and signaling.";
RL PLoS ONE 4:E7973-E7973(2009).
CC -!- FUNCTION: Subunit of novel type of clathrin- or non-clathrin-associated
CC protein coat involved in targeting proteins from the trans-Golgi
CC network (TGN) to the endosomal-lysosomal system. {ECO:0000250}.
CC -!- SUBUNIT: Adaptor protein complex 4 (AP-4) is a heterotetramer composed
CC of two large adaptins (epsilon-type subunit and beta-type subunit), a
CC medium adaptin (mu-type subunit) and a small adaptin (sigma-type
CC subunit) (By similarity). Interacts with EHD2 (PubMed:19936242).
CC {ECO:0000250|UniProtKB:Q9Y587, ECO:0000269|PubMed:19936242}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000250}. Membrane, coated pit {ECO:0000250}. Note=Associated with
CC the trans-Golgi network. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC005169; AAC62137.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06926.1; -; Genomic_DNA.
DR EMBL; AF370185; AAK44000.1; -; mRNA.
DR EMBL; AY059143; AAL15249.1; -; mRNA.
DR EMBL; AY088273; AAM65813.1; -; mRNA.
DR PIR; B84581; B84581.
DR RefSeq; NP_179569.1; NM_127537.4.
DR AlphaFoldDB; O82201; -.
DR SMR; O82201; -.
DR STRING; 3702.O82201; -.
DR iPTMnet; O82201; -.
DR PaxDb; 3702-AT2G19790-1; -.
DR ProteomicsDB; 244406; -.
DR EnsemblPlants; AT2G19790.1; AT2G19790.1; AT2G19790.
DR GeneID; 816498; -.
DR Gramene; AT2G19790.1; AT2G19790.1; AT2G19790.
DR KEGG; ath:AT2G19790; -.
DR Araport; AT2G19790; -.
DR TAIR; AT2G19790; AP4S.
DR eggNOG; KOG0934; Eukaryota.
DR HOGENOM; CLU_061221_4_0_1; -.
DR InParanoid; O82201; -.
DR OMA; GHVVETN; -.
DR OrthoDB; 21148at2759; -.
DR PhylomeDB; O82201; -.
DR PRO; PR:O82201; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82201; baseline and differential.
DR Genevisible; O82201; AT.
DR GO; GO:0030124; C:AP-4 adaptor complex; IPI:TAIR.
DR GO; GO:0005905; C:clathrin-coated pit; IEA:UniProtKB-KW.
DR GO; GO:0005802; C:trans-Golgi network; IDA:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR CDD; cd14832; AP4_sigma; 1.
DR Gene3D; 3.30.450.60; -; 1.
DR InterPro; IPR016635; AP_complex_ssu.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR PANTHER; PTHR11753; ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY; 1.
DR PANTHER; PTHR11753:SF4; CLAT_ADAPTOR_S DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR PIRSF; PIRSF015588; AP_complex_sigma; 1.
DR SUPFAM; SSF64356; SNARE-like; 1.
PE 1: Evidence at protein level;
KW Coated pit; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..143
FT /note="AP-4 complex subunit sigma"
FT /id="PRO_0000397860"
SQ SEQUENCE 143 AA; 16921 MW; B5A3672313F48623 CRC64;
MGIRFILMVN KQGQTRLAQY YEWLTLEERR ALEGEIVRKC LARNDQQCSF VEHRNYKIVY
RRYASLFFMV GVDDDENELA ILEFIHLLVE TMDKHFGNVC ELDIMFHLEK AHFMLEEMVM
NGCIVETSKA NILSPIQLMD KAH
//
