ID ARMC3_MOUSE Reviewed; 881 AA.
AC A2AU72; Q8BY67;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 27-MAR-2024, entry version 108.
DE RecName: Full=Armadillo repeat-containing protein 3;
GN Name=Armc3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INTERACTION WITH
RP PIK3C3; PIK3R4; BECN1 AND ATG14, PALMITOYLATION AT CYS-507 AND CYS-518, AND
RP MUTAGENESIS OF CYS-507 AND CYS-518.
RX PubMed=34428398; DOI=10.1016/j.devcel.2021.07.015;
RA Lei Y., Zhang X., Xu Q., Liu S., Li C., Jiang H., Lin H., Kong E., Liu J.,
RA Qi S., Li H., Xu W., Lu K.;
RT "Autophagic elimination of ribosomes during spermiogenesis provides energy
RT for flagellar motility.";
RL Dev. Cell 56:2313-2328(2021).
CC -!- FUNCTION: Essential for male fertility and sperm motility. During
CC spermatogenesis, promotes the autophagic degradation of excessive
CC ribosomes, providing energy resources for mitochondria and thus
CC ensuring sperm flagellar motility. {ECO:0000269|PubMed:34428398}.
CC -!- SUBUNIT: Homodimer (By similarity). Interacts with PIK3C3, PIK3R4 and
CC BECN1. Interacts (via ARM domains) with ATG14 (PubMed:34428398).
CC {ECO:0000250|UniProtKB:Q5W041, ECO:0000269|PubMed:34428398}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2AU72-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2AU72-2; Sequence=VSP_024502, VSP_024503, VSP_024504;
CC -!- TISSUE SPECIFICITY: Testis-specific. {ECO:0000269|PubMed:34428398}.
CC -!- PTM: Palmitoylation is important for its function in autophagy.
CC {ECO:0000269|PubMed:34428398}.
CC -!- DISRUPTION PHENOTYPE: Mice have normal viability but show complete male
CC infertility. Autophagic degradation of cytosolic ribosomes is blocked
CC in ARMC3-deficient spermatids, which causes low energy levels of
CC mitochondria and motionless sperm flagella.
CC {ECO:0000269|PubMed:34428398}.
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DR EMBL; AK041744; BAC31050.1; -; mRNA.
DR EMBL; AL929034; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS38052.1; -. [A2AU72-1]
DR RefSeq; NP_001074552.1; NM_001081083.2. [A2AU72-1]
DR AlphaFoldDB; A2AU72; -.
DR SMR; A2AU72; -.
DR STRING; 10090.ENSMUSP00000110287; -.
DR PhosphoSitePlus; A2AU72; -.
DR SwissPalm; A2AU72; -.
DR MaxQB; A2AU72; -.
DR PaxDb; 10090-ENSMUSP00000110287; -.
DR ProteomicsDB; 277292; -. [A2AU72-1]
DR ProteomicsDB; 277293; -. [A2AU72-2]
DR Antibodypedia; 44327; 106 antibodies from 18 providers.
DR Ensembl; ENSMUST00000114640.9; ENSMUSP00000110287.3; ENSMUSG00000037683.15. [A2AU72-1]
DR GeneID; 70882; -.
DR KEGG; mmu:70882; -.
DR UCSC; uc008imd.2; mouse. [A2AU72-1]
DR AGR; MGI:1918132; -.
DR CTD; 219681; -.
DR MGI; MGI:1918132; Armc3.
DR VEuPathDB; HostDB:ENSMUSG00000037683; -.
DR eggNOG; KOG0167; Eukaryota.
DR GeneTree; ENSGT00940000157476; -.
DR InParanoid; A2AU72; -.
DR OMA; LYPMQSR; -.
DR OrthoDB; 3152378at2759; -.
DR PhylomeDB; A2AU72; -.
DR TreeFam; TF329738; -.
DR BioGRID-ORCS; 70882; 0 hits in 75 CRISPR screens.
DR ChiTaRS; Armc3; mouse.
DR PRO; PR:A2AU72; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; A2AU72; Protein.
DR Bgee; ENSMUSG00000037683; Expressed in spermatid and 48 other cell types or tissues.
DR ExpressionAtlas; A2AU72; baseline and differential.
DR Genevisible; A2AU72; MM.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:UniProtKB.
DR GO; GO:0034517; P:ribophagy; IMP:UniProtKB.
DR GO; GO:0007286; P:spermatid development; IMP:UniProtKB.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 3.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR PANTHER; PTHR46618; ARMADILLO REPEAT-CONTAINING PROTEIN 3; 1.
DR PANTHER; PTHR46618:SF1; ARMADILLO REPEAT-CONTAINING PROTEIN 3; 1.
DR Pfam; PF00514; Arm; 4.
DR Pfam; PF14381; EDR1; 1.
DR SMART; SM00185; ARM; 9.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR PROSITE; PS50176; ARM_REPEAT; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Autophagy; Differentiation; Lipoprotein; Palmitate;
KW Reference proteome; Repeat; Spermatogenesis.
FT CHAIN 1..881
FT /note="Armadillo repeat-containing protein 3"
FT /id="PRO_0000284404"
FT REPEAT 15..54
FT /note="ARM 1"
FT REPEAT 57..96
FT /note="ARM 2"
FT REPEAT 98..138
FT /note="ARM 3"
FT REPEAT 140..179
FT /note="ARM 4"
FT REPEAT 181..220
FT /note="ARM 5"
FT REPEAT 222..262
FT /note="ARM 6"
FT REPEAT 264..304
FT /note="ARM 7"
FT REPEAT 306..345
FT /note="ARM 8"
FT REPEAT 346..385
FT /note="ARM 9"
FT REPEAT 388..427
FT /note="ARM 10"
FT REPEAT 429..468
FT /note="ARM 11"
FT REPEAT 470..509
FT /note="ARM 12"
FT REGION 605..659
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 624..639
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 507
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000269|PubMed:34428398"
FT LIPID 518
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000269|PubMed:34428398"
FT VAR_SEQ 644
FT /note="N -> NFRFS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024502"
FT VAR_SEQ 676..680
FT /note="SKKKS -> RKGKG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024503"
FT VAR_SEQ 681..881
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024504"
FT MUTAGEN 507
FT /note="C->A: Reduced level of palmitoylation and inability
FT to rescue the autophagic flux in ARMC3-deficient embryonic
FT fibrobalsts; when associated with A-518."
FT /evidence="ECO:0000269|PubMed:34428398"
FT MUTAGEN 518
FT /note="C->A: Reduced level of palmitoylation and inability
FT to rescue the autophagic flux in ARMC3-deficient embryonic
FT fibrobalsts; when associated with A-507."
FT /evidence="ECO:0000269|PubMed:34428398"
SQ SEQUENCE 881 AA; 97357 MW; 6898CE3C8F2C10D7 CRC64;
MGKKIKKEVE PPPKDVFDPI TIESKKAATV VLMLKSPEED ILAKACEAIY KFALKGEENK
ATLLELGAVE PLTKLLTHED KTVRRNAMMI FGILASNSDV KKLLRELEVM NSVIAQLSPE
EEVVIHEFAS LCLANMSVEY TGKVQIFEHG GLEPLIRLLS SSDPDVKKNS IECIYNLVQD
FQCRTTLQEL NAIPPILELL RSEYPIIQLL ALKTLGVITC DKEARTMLKE NQGLDHLTKI
LETKELNDLH VEALSVIANC LEDMDTMVLM QQTGSLKKVL SFAESSTIPD IQKNAAKAIA
KAAYDPENRK VFHEQEVEKC LVTLLGSDSD GTKIAASQAI SALCENLSCK EFFNTQGIPQ
IVQLLRSDNE EVREAAALAL ANLTTSSPAN ANAAAEADAI DPLINILSSK RDGAIANAAT
VLTNMATQEP LRAIIQNHEI MHALLGPLHS TNTLVQSTAA LTVAATACDV EARTQLRNCG
GLVPLVGLLH SKNDEVRRHA SWAVMVCAGD EPMAVELCRL GALNILEEIN RSLSRKNKFS
EAAYNKLLNN NLSLKYSQTG YLSSSNIISD GFYDYGRINP GTKLLSLKEL CLQELNDQRA
ILLVNNKSDT SPPPSMEDKS SDVGYGRSIS SSSSLRRGSK EKANAIFGSP TEEKSEPASV
RNTILSRAFT KEKGVSKKKS RLQLICSSYL LWKGKGKKEE EKVKEEEEIL ALPKFTEGSP
EKEWNPPPDP EFCVYVLEVT KSILPIVNLK EQIEVLAKYV ADKMGGKIPK EKLADFSWEL
HISELKFQLK SNVVPIGYIK KGIFYHRALL FKALADKIGV GCSLVRGEYS RGWNEVKLVN
EARKGMIGNL PPPEEYIVDL MFHPGNLLKL RSKEADLYRF L
//
