ID B0JQI1_MICAN Unreviewed; 998 AA.
AC B0JQI1;
DT 18-MAR-2008, integrated into UniProtKB/TrEMBL.
DT 18-MAR-2008, sequence version 1.
DT 27-MAR-2024, entry version 75.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=MAE_08180 {ECO:0000313|EMBL:BAG00640.1};
OS Microcystis aeruginosa (strain NIES-843 / IAM M-2473).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Microcystaceae; Microcystis.
OX NCBI_TaxID=449447 {ECO:0000313|EMBL:BAG00640.1, ECO:0000313|Proteomes:UP000001510};
RN [1] {ECO:0000313|EMBL:BAG00640.1, ECO:0000313|Proteomes:UP000001510}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-843 {ECO:0000313|EMBL:BAG00640.1,
RC ECO:0000313|Proteomes:UP000001510};
RX PubMed=18192279; DOI=10.1093/dnares/dsm026;
RA Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M.,
RA Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A.,
RA Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M.,
RA Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.;
RT "Complete genomic structure of the bloom-forming toxic cyanobacterium
RT Microcystis aeruginosa NIES-843.";
RL DNA Res. 14:247-256(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; AP009552; BAG00640.1; -; Genomic_DNA.
DR RefSeq; WP_012264362.1; NC_010296.1.
DR AlphaFoldDB; B0JQI1; -.
DR STRING; 449447.MAE_08180; -.
DR REBASE; 132278; Mae843ORF8180P.
DR PaxDb; 449447-MAE_08180; -.
DR EnsemblBacteria; BAG00640; BAG00640; MAE_08180.
DR KEGG; mar:MAE_08180; -.
DR PATRIC; fig|449447.4.peg.758; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_002151_1_1_3; -.
DR BioCyc; MAER449447:MAE_RS03650-MONOMER; -.
DR Proteomes; UP000001510; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR041635; Type_ISP_LLaBIII_C.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF18135; Type_ISP_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:BAG00640.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:BAG00640.1}.
FT DOMAIN 282..467
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 652..978
FT /note="Type ISP restriction-modification enzyme LLaBIII C-
FT terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF18135"
SQ SEQUENCE 998 AA; 117719 MW; 4AB723572C1FA950 CRC64;
MSRLLISQYQ AEVEKIVQYG GSRKETSIRV AFQNLLNDYC KARDFLLIPE LDYRTKSGKV
VYPDGTVKDA LRLDWGYWES KDQYDNLDEE IEKKLAKGYP NDNILFEDSQ TAVLIQGGEE
RLRVSMRDDE ALDGIINAFI NYVRPEVEDF REAIDSFKED LPTILEALRD LIALQSETNR
NFVTARDNFL EICRKSINPE ISLEDVREMI IQHILTEDIF INIFNESQFH RENNIARELQ
GVIETFFTGN TKRNTLGTIE RYYAVIRRTA ANIYNHHEKQ KFLKAIYENF YKAYNPKAAD
RLGIVYTPNE IVRFMIESVD YLVHKHFRKL LADPGVEILD PATGTGTFIT ELIEYLPKDK
LRYKYKHEMH CNEVAILPYY IANLNIEFTY KQKMGEYEEF EHICFVDTLD HAAFHLKQMD
LFAMSVENTQ RIQNQNDRNI SVIIGNPPYN ANQQNENDNN KNRKYPAIDK RIKDTYIEES
TAQKTKLYDM YSRFFRWATD RLGENGIIAF ITNSSFIDAR TFDGFRKVVE NEFSEIYIID
LGGNVRKNPK LSGTTHNVFG IQTGVAISLI VKRESNNLPC RILYTRRPEL DTASQKLEFL
SSTKLNQLDF EHIIPDKKHN WIEQSDNDFN DLIPVVDKNT KLLKNKTDIQ ALFEFFSLGV
STNRDEWVFE DDEQLLSKKM QYFISIYNKS IECNHINYSI KWSSSLISKF KNKEKSEYFP
RFVISLIYRP YITKYYYSNK FFSDRLTSNH YQVFGNELIN SNQVIMFSGV GSSKPNSVLV
TNKIFCLDTL EKTQCLPLYY YEKEGNRIDN ITDWGLQQFQ NHYNDKNLTK LDIFHYTYAV
LHYPEYRSKY ELNLKREFPR LPFYDNFSQW VEWGSKLMEL HINYETVAPY PLTRIDTNNN
LKPKTKLKAD REKNSINLDD VTFLQDIPKI AWEYKLGNRS ALEWILDQYK EKKPKDQTIA
ERFNNYRFAD YKETVIDLLQ RVCTVSVETM KIIEAMRH
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