ID B0TFJ5_HELMI Unreviewed; 440 AA.
AC B0TFJ5;
DT 08-APR-2008, integrated into UniProtKB/TrEMBL.
DT 08-APR-2008, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=Phenylacetate-coenzyme A ligase {ECO:0000256|PIRNR:PIRNR006444};
DE EC=6.2.1.30 {ECO:0000256|PIRNR:PIRNR006444};
DE AltName: Full=Phenylacetyl-CoA ligase {ECO:0000256|PIRNR:PIRNR006444};
GN Name=paaK {ECO:0000313|EMBL:ABZ83094.1};
GN ORFNames=HM1_0480 {ECO:0000313|EMBL:ABZ83094.1};
OS Heliobacterium modesticaldum (strain ATCC 51547 / Ice1).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Heliobacteriaceae;
OC Heliomicrobium.
OX NCBI_TaxID=498761 {ECO:0000313|EMBL:ABZ83094.1, ECO:0000313|Proteomes:UP000008550};
RN [1] {ECO:0000313|EMBL:ABZ83094.1, ECO:0000313|Proteomes:UP000008550}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51547 / Ice1 {ECO:0000313|Proteomes:UP000008550};
RX PubMed=18441057; DOI=10.1128/JB.00299-08;
RA Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M.,
RA Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E.,
RA Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T.,
RA Raymond J., Chen M., Blankenship R.E., Touchman J.W.;
RT "The genome of Heliobacterium modesticaldum, a phototrophic representative
RT of the Firmicutes containing the simplest photosynthetic apparatus.";
RL J. Bacteriol. 190:4687-4696(2008).
CC -!- FUNCTION: Catalyzes the activation of phenylacetic acid (PA) to
CC phenylacetyl-CoA (PA-CoA). {ECO:0000256|PIRNR:PIRNR006444}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-phenylacetate + ATP + CoA = AMP + diphosphate +
CC phenylacetyl-CoA; Xref=Rhea:RHEA:20956, ChEBI:CHEBI:18401,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57390, ChEBI:CHEBI:456215; EC=6.2.1.30;
CC Evidence={ECO:0000256|PIRNR:PIRNR006444};
CC -!- PATHWAY: Aromatic compound metabolism; phenylacetate degradation.
CC {ECO:0000256|PIRNR:PIRNR006444}.
CC -!- SIMILARITY: Belongs to the phenylacetyl-CoA ligase family.
CC {ECO:0000256|PIRNR:PIRNR006444}.
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DR EMBL; CP000930; ABZ83094.1; -; Genomic_DNA.
DR AlphaFoldDB; B0TFJ5; -.
DR STRING; 498761.HM1_0480; -.
DR KEGG; hmo:HM1_0480; -.
DR eggNOG; COG1541; Bacteria.
DR HOGENOM; CLU_035301_1_1_9; -.
DR UniPathway; UPA00930; -.
DR Proteomes; UP000008550; Chromosome.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0047475; F:phenylacetate-CoA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0010124; P:phenylacetate catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd05913; PaaK; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR028154; AMP-dep_Lig_C.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR011880; PA_CoA_ligase.
DR PANTHER; PTHR43845; BLR5969 PROTEIN; 1.
DR PANTHER; PTHR43845:SF1; BLR5969 PROTEIN; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF14535; AMP-binding_C_2; 1.
DR PIRSF; PIRSF006444; PaaK; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|PIRNR:PIRNR006444, ECO:0000313|EMBL:ABZ83094.1};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR006444};
KW Reference proteome {ECO:0000313|Proteomes:UP000008550}.
FT DOMAIN 85..291
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 340..430
FT /note="AMP-dependent ligase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14535"
SQ SEQUENCE 440 AA; 49484 MW; 233C969FD4FDA0E9 CRC64;
MTPSAVACER TEMRRPIDFD AIAQSQFPLL VQTIRRAYQM SPFYRERFQA AGIAPDHIRS
MDDLARLPFT TKQDLRQAYP LGLMAVNEEE VVRIHSSSGT TGKPVIVPYT RKDVDDWAVM
MARCLKTVGV SRRDRVQVTP GYGLWTAGIG FQAGVEKLGA MTIPTGPGNT DKQLEMMTDL
KASVLIGTSS YGLLLAEEAE RRGILEAIQL RIGIFGSERW GDKMRRRIEE IFGIETFDIY
GLTEIYGPGI AIDCPCHEGL HFWSDYLIFE VIDPETGHVL PPGQEGELVI TTIAKEGMPL
IRYRTRDITT IETYACSCGS PFPMIRRVLG RTDDMIKIKG VNIYPGQIDH VLKHTPGVSS
EYQIVLERVD NKDHIRIRVE LSNGHEPEDV ARACRKNIKS VIGIVADVEV LPHGGLPRSE
KKTRRVFDHR YDYLENPPKA
//
