GenomeNet

Database: UniProt
Entry: B0UT76
LinkDB: B0UT76
Original site: B0UT76 
ID   RIMK_HISS2              Reviewed;         299 AA.
AC   B0UT76;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   13-FEB-2019, entry version 70.
DE   RecName: Full=Probable alpha-L-glutamate ligase {ECO:0000255|HAMAP-Rule:MF_01552};
DE            EC=6.3.2.- {ECO:0000255|HAMAP-Rule:MF_01552};
GN   Name=rimK {ECO:0000255|HAMAP-Rule:MF_01552};
GN   OrderedLocusNames=HSM_0995;
OS   Histophilus somni (strain 2336) (Haemophilus somnus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Histophilus.
OX   NCBI_TaxID=228400;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2336;
RG   US DOE Joint Genome Institute;
RA   Siddaramappa S., Duncan A.J., Challacombe J.F., Rainey D.,
RA   Gillaspy A.F., Carson M., Gipson J., Gipson M., Bruce D., Detter J.C.,
RA   Han C.S., Land M., Tapia R., Thompson L.S., Orvis J., Zaitshik J.,
RA   Barnes G., Brettin T.S., Dyer D.W., Inzana T.J.;
RT   "Complete sequence of Haemophilus somnus 2336.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01552};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01552};
CC       Note=Binds 2 magnesium or manganese ions per subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_01552};
CC   -!- SIMILARITY: Belongs to the RimK family. {ECO:0000255|HAMAP-
CC       Rule:MF_01552}.
DR   EMBL; CP000947; ACA32682.1; -; Genomic_DNA.
DR   RefSeq; WP_012341793.1; NC_010519.1.
DR   SMR; B0UT76; -.
DR   PRIDE; B0UT76; -.
DR   EnsemblBacteria; ACA32682; ACA32682; HSM_0995.
DR   GeneID; 31487293; -.
DR   KEGG; hsm:HSM_0995; -.
DR   HOGENOM; HOG000293092; -.
DR   KO; K05844; -.
DR   OMA; NYLRCYM; -.
DR   OrthoDB; 937913at2; -.
DR   BioCyc; HSOM228400:G1GB8-1045-MONOMER; -.
DR   Proteomes; UP000008543; Chromosome.
DR   GO; GO:0016881; F:acid-amino acid ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006464; P:cellular protein modification process; IEA:InterPro.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   HAMAP; MF_01552; RimK; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013651; ATP-grasp_RimK-type.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR023533; RimK.
DR   InterPro; IPR004666; RpS6_RimK/Lys_biosynth_LsyX.
DR   Pfam; PF08443; RimK; 1.
DR   TIGRFAMs; TIGR00768; rimK_fam; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN         1    299       Probable alpha-L-glutamate ligase.
FT                                /FTId=PRO_1000087746.
FT   DOMAIN      112    294       ATP-grasp. {ECO:0000255|HAMAP-
FT                                Rule:MF_01552}.
FT   NP_BIND     185    186       ATP. {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   NP_BIND     218    220       ATP. {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   METAL       255    255       Magnesium or manganese 1.
FT                                {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   METAL       267    267       Magnesium or manganese 1.
FT                                {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   METAL       267    267       Magnesium or manganese 2.
FT                                {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   METAL       269    269       Magnesium or manganese 2.
FT                                {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   BINDING     148    148       ATP. {ECO:0000255|HAMAP-Rule:MF_01552}.
FT   BINDING     194    194       ATP. {ECO:0000255|HAMAP-Rule:MF_01552}.
SQ   SEQUENCE   299 AA;  33344 MW;  311D46291643BB4F CRC64;
     MKLLMLCREP RLYSCQRLKE SAEDNGHQID ILDPNRCLLK LSKNAPHFEL YYQVNSKSEP
     YLLPDYDAII PRFGSTSTRM GCAVLRHFRT KNVFCLNDDV AFLKARDKWL SLQLLTEQGI
     AVPNSALSGA EFSATQAILQ IQSPTILKTL HGSQGIGVIL AENRKSAVSI METLTQADVP
     LLMQDFIQEA QGTDIRCFVI GDKVVATMQR IGQEDEFRAN FHRGGSAEKI QLTEQEKVLA
     LKATKCLGLD VAGVDLIRSK QGLLVLEVNA SPGLEMIEKT SGIDIALQMI VHIEKQFKR
//
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