GenomeNet

Database: UniProt
Entry: B0XDC4
LinkDB: B0XDC4
Original site: B0XDC4 
ID   SPNE_CULQU              Reviewed;        1396 AA.
AC   B0XDC4;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   31-JUL-2019, entry version 64.
DE   RecName: Full=Probable ATP-dependent RNA helicase spindle-E;
DE            EC=3.6.4.13;
GN   Name=spn-E; ORFNames=CPIJ017541;
OS   Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
OC   Pterygota; Neoptera; Holometabola; Diptera; Nematocera; Culicoidea;
OC   Culicidae; Culicinae; Culicini; Culex; Culex.
OX   NCBI_TaxID=7176;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JHB;
RG   The Broad Institute Genome Sequencing Platform;
RA   Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.D.,
RA   Hannick L.I., Megy K., O'Leary S.B., Pearson M., Haas B.J.,
RA   Mauceli E., Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L.,
RA   Amedeo P., Antoine C.H., Arensburger P., Bidwell S.L., Crawford M.,
RA   Camaro F., Devon K., Engels R., Hammond M., Howarth C., Koehrsen M.,
RA   Lawson D., Montgomery P., Nene V., Nusbaum C., Puiu D.,
RA   Romero-Severson J., Severson D.W., Shumway M., Sisk P., Stolte C.,
RA   Zeng Q., Eisenstadt E., Fraser-Liggett C.M., Strausberg R.,
RA   Galagan J., Birren B., Collins F.H.;
RT   "Annotation of Culex pipiens quinquefasciatus.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable ATP-binding RNA helicase which plays a central
CC       role during gametogenesis by repressing transposable elements and
CC       preventing their mobilization, which is essential for the germline
CC       integrity. Acts via the piRNA metabolic process, which mediates
CC       the repression of transposable elements during meiosis by forming
CC       complexes composed of piRNAs and Piwi proteins and govern the
CC       methylation and subsequent repression of transposons (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=Component of
CC       the nuage, also named P granule, a germ-cell-specific organelle
CC       required to repress transposon during meiosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH
CC       subfamily. {ECO:0000305}.
DR   EMBL; DS232752; EDS45402.1; -; Genomic_DNA.
DR   RefSeq; XP_001867646.1; XM_001867611.1.
DR   SMR; B0XDC4; -.
DR   STRING; 7176.CPIJ017541-PA; -.
DR   PRIDE; B0XDC4; -.
DR   EnsemblMetazoa; CPIJ017541-RA; CPIJ017541-PA; CPIJ017541.
DR   GeneID; 6051164; -.
DR   KEGG; cqu:CpipJ_CPIJ017541; -.
DR   VectorBase; CPIJ017541-RA; CPIJ017541-PA; CPIJ017541.
DR   eggNOG; KOG0920; Eukaryota.
DR   eggNOG; COG1643; LUCA.
DR   HOGENOM; HOG000047965; -.
DR   InParanoid; B0XDC4; -.
DR   KO; K18408; -.
DR   OMA; DPCRTVY; -.
DR   OrthoDB; 278674at2759; -.
DR   PhylomeDB; B0XDC4; -.
DR   Proteomes; UP000002320; Partially assembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0007275; P:multicellular organism development; IEA:UniProtKB-KW.
DR   GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.90; -; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR035437; SNase_OB-fold_sf.
DR   InterPro; IPR002999; Tudor.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00567; TUDOR; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50304; TUDOR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Developmental protein;
KW   Differentiation; Helicase; Hydrolase; Meiosis; Nucleotide-binding;
KW   Reference proteome; RNA-mediated gene silencing; Spermatogenesis.
FT   CHAIN         1   1396       Probable ATP-dependent RNA helicase
FT                                spindle-E.
FT                                /FTId=PRO_0000391912.
FT   DOMAIN       68    234       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      292    468       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   DOMAIN      885    950       Tudor. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00211}.
FT   NP_BIND      81     88       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       180    183       DEAH box.
SQ   SEQUENCE   1396 AA;  158391 MW;  2E06F4195481C69C CRC64;
     MDEAAGPSTS RTSNLEDVDD EGASLAEEDE EHTKALKAKE MMAPLFQRYN FTMKKNDLPI
     NWNKPEILDK IRSNAVVVLQ GATGCGKTTQ VPQYLLEEAF ERKEYCNIIV TQPRKIAAIS
     IARRVAQERK CDLGTLVGYK VGLKEQHNED TRLLYVTTGV LLQSLINSKT MATYTHVILD
     EVHEREVDMD FLLIVVRRLL STNSKKTKVI LMSATIDAKG FSEYFKIPKK SGYLSAPVIS
     VERPRLHVVQ EFYFDDLEKL KVDFQIDYEA PGISEQMYNI AAKLVVVCDH LKGQEFGDSL
     EYKPSIIIFL PGINEIEKME GALEKLIASI QNAAQRPNLL IMKLHSTLPA DDQTAVFRKP
     GPNQRKVILS TNIAESSITV PDIKFVIDFC LQRILVTDTL TNFSTLRTEW ASKSNCIQRA
     GRAGRLMSGR VYRLVDRRFF ENNMDVSTSP EILRCPLETV VLKAKLLEMG TPPSILALAM
     APPNLDDIRN TILLLKEVGA MLRTVKGNYD QLDGDLTYLG RIMSKLPLDI RISKLIMLGY
     IFSVMEEAVT IGAGMNVKNI FLNQNSVKTY SQKMYWADGS GSDAIAILNA YTAWKSRQEQ
     AGDTADMYNW ARRMSLDRKS LIDMAELIHE VKDRLNRSGL KPVSGPNRVV WNAREKTVIL
     KVILAGAFYP NYFIPMSVGG KELMERQSFT ELGGRDPCNT VFFTGFDHER YIGPLYTVQI
     KKILSEGDYS KHQAMKVMYD RTTNRIFVTF LGTTDERDQR GSFMPGKVHA DVYRAIKLRK
     LGARNRITEI RTMRQRDAID YATSAGLGHW EDANGWVPRR KIVRNAHLSV LPPIFREKVV
     CQVTHVVHPN KFFLRPEDNR NKDIFREIHT QLNARQLHPF PADANFAMGQ MVAAPVQENQ
     ETYARAVLRS YRNVRTTGSV SWTVFFIDYG HTAAMEETAF RQLDDSLGQL KDIPPRAFEA
     TLTEIQPSAI ISPQGSWTTE SIHRFKELVL GKIFVAKVYS VVGVVASVEL SRDEIRMNSE
     LIRLKYAQYA EESYISKLDH DHRERKQREI MMDENLRNEV YRSAELTQNT YEDDELEDVN
     PPEDKLRCKV VLSGPHSPLE TSASATIRSS VMKPVSIESD SVNSILLDSN PQDTHEKLLV
     AGGVNEQGNR LVLRQTSVMP NIPGFGAIMS LIFCPTAQLK KDKDETRVVT VLSGLGYDPS
     TGEALYPEHD MALTLDVVLN DDDITNINAL RYTMDSILHT GEGQTDPKFG DASIQKLKLQ
     VKQYIIKILE HERRFLDLRH APNDYNWRCD LNLTAGSSSS KKMKGDFNIY DKKAIFPLLE
     PLNLLPVSAS QLTFLKKHCH ELHKLAHTDV QLPRHGITCQ LCNNVLETLP QLRIHLYSKL
     HRDRESQIKY RSPQMY
//
DBGET integrated database retrieval system