ID B1GYH0_NICSY Unreviewed; 1558 AA.
AC B1GYH0;
DT 29-APR-2008, integrated into UniProtKB/TrEMBL.
DT 29-APR-2008, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=DNA (cytosine-5)-methyltransferase {ECO:0000256|PIRNR:PIRNR037404};
DE EC=2.1.1.37 {ECO:0000256|PIRNR:PIRNR037404};
GN Name=met1 {ECO:0000313|EMBL:CAQ18900.1};
GN Synonyms=LOC104234926 {ECO:0000313|RefSeq:XP_009786876.1};
OS Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4096 {ECO:0000313|EMBL:CAQ18900.1};
RN [1] {ECO:0000313|EMBL:CAQ18900.1}
RP NUCLEOTIDE SEQUENCE.
RA Fulnecek J., Matyasek R., Kovarik A.;
RT "Faithful inheritance of cytosine methylation patterns in repeated
RT sequences of the allotetraploid tobacco correlates with the expression of
RT DNA methyltransferase gene families from both parental genomes.";
RL Mol. Genet. Genomics 281:407-420(2009).
RN [2] {ECO:0000313|Proteomes:UP000189701}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23773524; DOI=10.1186/gb-2013-14-6-r60;
RA Sierro N., Battey J.N., Ouadi S., Bovet L., Goepfert S., Bakaher N.,
RA Peitsch M.C., Ivanov N.V.;
RT "Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana
RT tomentosiformis.";
RL Genome Biol. 14:R60.1-R60.17(2013).
RN [3] {ECO:0000313|RefSeq:XP_009786876.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_009786876.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:13681, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:11370,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:85454; EC=2.1.1.37;
CC Evidence={ECO:0000256|PIRNR:PIRNR037404,
CC ECO:0000256|RuleBase:RU000417};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|PIRNR:PIRNR037404}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. C5-methyltransferase family.
CC {ECO:0000256|PIRNR:PIRNR037404, ECO:0000256|PROSITE-ProRule:PRU01016,
CC ECO:0000256|RuleBase:RU000416}.
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DR EMBL; AM946605; CAQ18900.1; -; mRNA.
DR RefSeq; NP_001289530.1; NM_001302601.1.
DR RefSeq; XP_009786876.1; XM_009788574.1.
DR STRING; 4096.B1GYH0; -.
DR GeneID; 104234926; -.
DR KEGG; nsy:104234926; -.
DR eggNOG; ENOG502QPKK; Eukaryota.
DR OrthoDB; 317994at2759; -.
DR Proteomes; UP000189701; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003682; F:chromatin binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR CDD; cd04712; BAH_DCM_I; 1.
DR CDD; cd04708; BAH_plantDCM_II; 1.
DR Gene3D; 2.30.30.490; -; 2.
DR Gene3D; 3.90.120.10; DNA Methylase, subunit A, domain 2; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR001025; BAH_dom.
DR InterPro; IPR043151; BAH_sf.
DR InterPro; IPR018117; C5_DNA_meth_AS.
DR InterPro; IPR001525; C5_MeTfrase.
DR InterPro; IPR031303; C5_meth_CS.
DR InterPro; IPR022702; Cytosine_MeTrfase1_RFD.
DR InterPro; IPR017198; DNMT1-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR00675; dcm; 1.
DR PANTHER; PTHR10629; CYTOSINE-SPECIFIC METHYLTRANSFERASE; 1.
DR PANTHER; PTHR10629:SF52; DNA (CYTOSINE-5)-METHYLTRANSFERASE 1; 1.
DR Pfam; PF01426; BAH; 2.
DR Pfam; PF00145; DNA_methylase; 1.
DR Pfam; PF12047; DNMT1-RFD; 2.
DR PIRSF; PIRSF037404; DNMT1; 5.
DR PRINTS; PR00105; C5METTRFRASE.
DR SMART; SM00439; BAH; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51038; BAH; 2.
DR PROSITE; PS00094; C5_MTASE_1; 1.
DR PROSITE; PS00095; C5_MTASE_2; 1.
DR PROSITE; PS51679; SAM_MT_C5; 1.
PE 2: Evidence at transcript level;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR037404};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000256|PIRNR:PIRNR037404};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR037404};
KW Reference proteome {ECO:0000313|Proteomes:UP000189701};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PIRNR:PIRNR037404};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR037404}.
FT DOMAIN 761..895
FT /note="BAH"
FT /evidence="ECO:0000259|PROSITE:PS51038"
FT DOMAIN 935..1075
FT /note="BAH"
FT /evidence="ECO:0000259|PROSITE:PS51038"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 295..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 691..737
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..55
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..309
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 320..340
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 691..710
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 711..737
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1225
FT /evidence="ECO:0000256|PIRSR:PIRSR037404-1,
FT ECO:0000256|PROSITE-ProRule:PRU01016"
SQ SEQUENCE 1558 AA; 175372 MW; B0FF953A68FCEB43 CRC64;
MGSLAGLDKP DTDAAGHKKG KSRQDSVSKR KAPATDKKEK KQPVSEAIEE PTAACKRPKR
AAACSNFKEK TVHLSKNSSV IETKKYQCVE EEVVAIRLTV GLQDSQRPCR RLTDFIFHNS
EGIPQPFEMS EVDDLFITGL ILPLEDNIDK EKAKGIRCEG FGRIEEWAIS GYEDGTPIIW
ISTETADYDC KKPSGGYKKF YDHFFAKATA CIEVYKKLSK SSGGNPDLSL DELLAGVVRA
MSGLKCFSGG VSIRDFLISQ GEFVYKQLIG LDDTSKKTDQ LFVELPVLAS LRDESSNQEM
LSQPEPLSFG KTLTIGPKVG KGEGKRDQSD LTTGPEQEEE DLKLAKLLHE QEYWHSLNQK
KSRSTSSSSS KFYIKINEDE IASDYPLPAY YKTCNEETDE YIVFDSGVDT YYIDDLPRSM
LHNWALYNSD SRLISLELLP MKPCADIDVT IFGSGVMTAD DGSGYNVDAD ANNSSSGGSG
SAEIDGMPIY LSAIKEWMIE FGSSMIFISI RTDMAWYRLG KPSKQYAPWY EPVLKTAKLA
VSIITLLKEQ SRCARLSFGD VIKRVSEFKK HHPAYISSNT DVVERYVVVH GQIILQQFSE
FPDESIRKCA FVIGLSRKME ERHHTKWLIK KKKVVQRHEQ NLNPRASMAP SVKRKAMQAT
TTRLINRIWG EYYSNYSPET SKEVVACEVK DDEEADEQEE NDEDDAQEEN LEVSEKTHTP
CSTRRHIKSR SDSKEINWDG ESIGKTASGE LLFKKARIHG NEIAVGDSVL VEHDEPDELP
SIYFVEYMFE KLDGSKMLHG RMMQRGSETV LGNAANEREV FLINECMDLQ LGDVKESVVV
SIRMMPWGHQ HRKANAYVDK LDRAKAEDRK KKGLPSEFYC KSFYQPDRGA FFRLPFDKMG
LGNGLCYSCE LQQIDQEKES FKLDMSNSSF VYLGTEYSID DFVYIHPDHF AVERGGSGTF
KAGRNVGLMA YVVCQLIEIS GPKGSKQAKV DSTNVKVRRF FRPEDISSDK AYSSDIREIY
YSEEIHTVPV ETIEGKCEVR KKYDIPSEDV PAIFDHVFFC EYFYDPLNGS LKQLPAQVKL
RFSRVKLDDA ASRKRKGKGK EGEDELRVGQ LNEASQQNRL ATLDIFAGCG GLSEGLQRSG
VSDTKWAIEY EEPAGDAFKL NHPEAKVFIQ NCNVFLRVVM QKCGDAEDCI STPEASELAA
AMDESELNSL PLPGQVDFIN GGPPCQGFSG MNRFNQSTWS KVQCEMILAF LSFADYYRPK
FFLLENVRNF VSFNQKQTFR LTVASLLEMG YQVRFGILEA GAFGVPQSRK RAFIWAASPE
EILPEWPEPM HVFGVPELKI ALSETSHYAA VRSTASGAPF RSLTVRDTIG DLPAVGNGAS
KTCIEYQVDP VSWFQRKIRG NSITLSDHIT KEMNELNLIR CQRIPKRPGA DWRDLPDEKV
KLSNGQLVDL IPWCLPNTAK RHNQWKGLFG RLDWDGNFPT SITDPQPMGK VGMCFHPDQD
RIVTVRECAR SQGFPDSYQF AGNILHKHRQ IGNAVPPPLA YALGRKLKEA VESNKRST
//
