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Database: UniProt
Entry: B1KJA5
LinkDB: B1KJA5
Original site: B1KJA5 
ID   RAPA_SHEWM              Reviewed;         968 AA.
AC   B1KJA5;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   31-JUL-2019, entry version 71.
DE   RecName: Full=RNA polymerase-associated protein RapA {ECO:0000255|HAMAP-Rule:MF_01821};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_01821};
DE   AltName: Full=ATP-dependent helicase HepA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   Name=rapA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   OrderedLocusNames=Swoo_4324;
OS   Shewanella woodyi (strain ATCC 51908 / MS32).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=392500;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51908 / MS32;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T.,
RA   Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Zhao J.-S., Richardson P.;
RT   "Complete sequence of Shewanella woodyi ATCC 51908.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription regulator that activates transcription by
CC       stimulating RNA polymerase (RNAP) recycling in case of stress
CC       conditions such as supercoiled DNA or high salt concentrations.
CC       Probably acts by releasing the RNAP, when it is trapped or
CC       immobilized on tightly supercoiled DNA. Does not activate
CC       transcription on linear DNA. Probably not involved in DNA repair.
CC       {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SUBUNIT: Interacts with the RNAP. Has a higher affinity for the
CC       core RNAP than for the holoenzyme. Its ATPase activity is
CC       stimulated by binding to RNAP. {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. RapA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01821}.
DR   EMBL; CP000961; ACA88577.1; -; Genomic_DNA.
DR   RefSeq; WP_012326903.1; NC_010506.1.
DR   STRING; 392500.Swoo_4324; -.
DR   EnsemblBacteria; ACA88577; ACA88577; Swoo_4324.
DR   KEGG; swd:Swoo_4324; -.
DR   eggNOG; ENOG4105BZK; Bacteria.
DR   eggNOG; COG0553; LUCA.
DR   HOGENOM; HOG000218482; -.
DR   KO; K03580; -.
DR   OMA; MSILERD; -.
DR   OrthoDB; 291634at2; -.
DR   BioCyc; SWOO392500:G1GBD-4750-MONOMER; -.
DR   Proteomes; UP000002168; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   HAMAP; MF_01821; Helicase_RapA; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR023949; Helicase_RapA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022737; RapA_C.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   InterPro; IPR040765; Tudor_1_RapA.
DR   InterPro; IPR040766; Tudor_2_RapA.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF12137; RapA_C; 1.
DR   Pfam; PF00176; SNF2_N; 1.
DR   Pfam; PF18339; Tudor_1_RapA; 1.
DR   Pfam; PF18337; Tudor_RapA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; Complete proteome; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    968       RNA polymerase-associated protein RapA.
FT                                /FTId=PRO_1000188192.
FT   DOMAIN      163    332       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   DOMAIN      491    645       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   NP_BIND     176    183       ATP. {ECO:0000255|HAMAP-Rule:MF_01821}.
FT   MOTIF       278    281       DEAH box.
SQ   SEQUENCE   968 AA;  108948 MW;  399BD68A087B4F62 CRC64;
     MPFSLGQRWI SDTESELGLG TVVAVEGRMV TVMFPATDEN RMFSRADAPL TRVIFNPGDK
     AESHEGWSLT VSEVEEKDNL IIYHGIHDET GEQVSLRETL LNHNIRFNKP QDRLFAGQID
     RLERFGIRYQ CQQLRHKLAT SDMLGLQGPR VGLIPHQQWI AHEVGRRFAP RVLLADEVGL
     GKTIEAGLII HQQLLTGRAE RILVIVPDTL RHQWLVEMLR RFNLKFSVFD EDRCVEAYAD
     SDNPFYTEQL VICSLELLRK KRRLDQALAA DWDLMVVDEA HHLEWTEDAP SRAYRIVEAL
     SEEIPGVLLL TATPDQLGHQ SHFARLRLLD PDRFYDYEAF LKEETNYADI ASTADALAGN
     EPLSQEIIDN LKTQLAEKDI TAATDIIQAT DADVDQQQAA RDALLQDLLD RHGTGRVLYR
     NSRASVKGFP TRIFNQYPQK MPAQYVTAAR VGAMMNGHLD TAGKVKQALS PEKIYQEFES
     SSASWWKFDP RVDWLIDFLK ENRREKVLII ASQAETALSL EEALRTREGI QATVFHEGMS
     IIERDKAGAY FAQETGGAQA LICSEIGSEG RNFQFASQLI LFDLPLNPDL LEQRIGRLDR
     IGQNNDVSIH VPYLEDTAQE SLMQWYHKGL NAFEQTCPSG HILFNEFSES LLNVLISQDK
     EVLEQVLSDT QTRYAELKSV MEQGRDKLLE INSHGGERAN KLVNALAERD EDTNLIGSVI
     RLWDIIGVEQ EDSGENAIVL RPSEHMMFPT YPGLPEDGIT VTFDREMALS RDDIALITQE
     HPLVQTGLDL ITSSETGTTS VAVLKNKSLP AGTIFLELIY MADASAPKSS QLYRYLPPTP
     VRVLLDKNGN NLADNVNYES FNKQLSAVNR HIASKLVNAS QAVLHPLFAK GEEFASSELT
     LLTESSRAKM TTQLNGELER LEALKAVNPN IRDEELAHLR EQMVELNGYL DGAVLQLDAI
     RLVLVSHA
//
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