ID B1ZPN9_OPITP Unreviewed; 711 AA.
AC B1ZPN9;
DT 20-MAY-2008, integrated into UniProtKB/TrEMBL.
DT 20-MAY-2008, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN OrderedLocusNames=Oter_2209 {ECO:0000313|EMBL:ACB75492.1};
OS Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1).
OC Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae; Opitutus.
OX NCBI_TaxID=452637 {ECO:0000313|EMBL:ACB75492.1, ECO:0000313|Proteomes:UP000007013};
RN [1] {ECO:0000313|EMBL:ACB75492.1, ECO:0000313|Proteomes:UP000007013}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 11246 / JCM 15787 / PB90-1
RC {ECO:0000313|Proteomes:UP000007013};
RX PubMed=21398538; DOI=10.1128/JB.00228-11;
RA van Passel M.W., Kant R., Palva A., Copeland A., Lucas S., Lapidus A.,
RA Glavina del Rio T., Pitluck S., Goltsman E., Clum A., Sun H., Schmutz J.,
RA Larimer F.W., Land M.L., Hauser L., Kyrpides N., Mikhailova N.,
RA Richardson P.P., Janssen P.H., de Vos W.M., Smidt H.;
RT "Genome sequence of the verrucomicrobium Opitutus terrae PB90-1, an
RT abundant inhabitant of rice paddy soil ecosystems.";
RL J. Bacteriol. 193:2367-2368(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR EMBL; CP001032; ACB75492.1; -; Genomic_DNA.
DR AlphaFoldDB; B1ZPN9; -.
DR STRING; 452637.Oter_2209; -.
DR CAZy; GH42; Glycoside Hydrolase Family 42.
DR KEGG; ote:Oter_2209; -.
DR eggNOG; COG1874; Bacteria.
DR HOGENOM; CLU_012430_1_0_0; -.
DR Proteomes; UP000007013; Chromosome.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006012; P:galactose metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013739; Beta_galactosidase_C.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08533; Glyco_hydro_42C; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084, ECO:0000313|EMBL:ACB75492.1};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084, ECO:0000313|EMBL:ACB75492.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000007013};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..711
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002774425"
FT DOMAIN 54..424
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 462..640
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT DOMAIN 652..708
FT /note="Beta-galactosidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08533"
FT ACT_SITE 189
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 346
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 150
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 188
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 711 AA; 79290 MW; 6D875EAC35F7D31C CRC64;
MLPRFLPIMT ALALASSLPA ANPSGQPGLA PSANRPPSHL RTSTFQEFHW GVAYYPEHGE
LATLDQDAER MLAAGMNTVR MAEFAWDLME PEEGRYDFSF FDDAIARLGA KGIRTILCTP
TAAPPRWLSL SHPEILRVDA RGVVQRHGSR QHASTASDVY RDYSRKITRA MAEHFANNPN
VIGWQTDNEF NCHFSEDYSA GAAQAWIGFL REKFHDDIAA LNRAWGTAFW AQTYARFEDV
PLPAAGQPTH LNPAHRLDYT RFIAWTVARF QHDQVEILRA AQPRWWITHN GMFRLVDYRG
QFGRDLDVLG CDVYPMFTWD PRQRGATQAW ALDAARAWTG NFFVPEHQSG PGGQTDYFHD
NPEPGELRRM LYTSFAHGAD SVLLFRWRTA RFGAEEYWCG VLDHDNVPRR RYAEVAQVGA
ELARVGPALL GTHVAIDTAI AATDFDNTNA HEALHFNLPA PDQVAAELHG HFWRAGYRVG
AVHPGDTLRG LKLYIIPHFT IIDTAWLPQL ESWVRDGGVL VIGARAGTKD ADNNVTTDLL
PGVLRPLVGA TVVEYGRQNA PEQRPLELEI AGKRVLSREW YEQLAPDDGT EVWLRWATRH
QTGTPAATLH RLGKGAVVYV GTYLNGAVGG ALVPELAKLA HVLPALAGAP AGVEVVRREA
DDRALWFLIN HTEQPQYVTG LPRGRDLVAD RDVRDELTLP PRGVAVIRTP R
//
