ID B1_USTMA Reviewed; 410 AA.
AC P22015; A0A0D1E8M0; Q4PH36;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 12-DEC-2006, sequence version 2.
DT 24-JAN-2024, entry version 121.
DE RecName: Full=Mating-type locus allele B1 protein;
GN ORFNames=UMAG_12052;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=1967554; DOI=10.1016/0092-8674(90)90744-y;
RA Schulz B., Banuett F., Dahl M., Schlesinger R., Schaefer W., Martin T.,
RA Herskowitz I., Kahmann R.;
RT "The b alleles of U. maydis, whose combinations program pathogenic
RT development, code for polypeptides containing a homeodomain-related
RT motif.";
RL Cell 60:295-306(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=2227416; DOI=10.1101/gad.4.8.1384;
RA Kronstad J.W., Leong S.A.;
RT "The b mating-type locus of Ustilago maydis contains variable and constant
RT regions.";
RL Genes Dev. 4:1384-1395(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=521 / FGSC 9021;
RA Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The B locus has at least 25 alleles, and any combination of
CC two different B alleles yields a multimeric regulatory protein, that
CC activates genes responsible for the pathogenicity and for the sexual
CC development of the fungus within the corn plant.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the TALE/M-ATYP homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=KIS72159.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; M58553; AAA63553.1; -; Genomic_DNA.
DR EMBL; X61305; CAA43602.1; -; Genomic_DNA.
DR EMBL; CM003140; KIS72159.1; ALT_SEQ; Genomic_DNA.
DR PIR; A32696; A32696.
DR PIR; A36671; A36671.
DR RefSeq; XP_011386823.1; XM_011388521.1.
DR AlphaFoldDB; P22015; -.
DR SMR; P22015; -.
DR STRING; 237631.P22015; -.
DR EnsemblFungi; KIS72159; KIS72159; UMAG_12052.
DR GeneID; 23567838; -.
DR KEGG; uma:UMAG_12052; -.
DR eggNOG; ENOG502RSBJ; Eukaryota.
DR HOGENOM; CLU_671208_0_0_1; -.
DR InParanoid; P22015; -.
DR OMA; LWFINAR; -.
DR OrthoDB; 1475673at2759; -.
DR Proteomes; UP000000561; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR008888; Ustilago_mating.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF05722; Ustilago_mating; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 3: Inferred from homology;
KW DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..410
FT /note="Mating-type locus allele B1 protein"
FT /id="PRO_0000049401"
FT DNA_BIND 107..184
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..110
FT /note="Variable domain between B alleles"
FT REGION 111..410
FT /note="Highly conserved between B alleles"
FT REGION 202..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 278..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 333..410
FT /note="Not essential for B1 function"
FT REGION 374..395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 276..308
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 202..229
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..335
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 168..169
FT /note="QL -> HV (in Ref. 1; AAA63553)"
FT /evidence="ECO:0000305"
FT CONFLICT 218
FT /note="S -> Y (in Ref. 1; AAA63553)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="K -> N (in Ref. 1; AAA63553)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 410 AA; 46197 MW; AF7AA3EC852F2985 CRC64;
MSSDPNFSLI SFLECLNEIE HEFLRDKGEN YPVLVRKLRE LQQKIPNDIA NLPRDPETIQ
QIHQTTHRIR AVAQAFIRFD QKFVSLCSEV VHGTSKVMQE FNVVSPDVGC RNLSEDLPAY
HMRKHFLLTL DNPYPTQEEK ETLVRLTNES TARVGQSSVN RPPLEVHQLT LWFINARRRS
GWSHILKKFA REDRSRMKHL VRAKLSSSNQ STPPSSTSDS LSNNLDDVLS DNLGRPLTPV
DKQQFEDDWA SMISWIKYGV KEKVGDWVYD LCAASKKTPK PGMPRPVTTV AKRHPARKTK
PAAKPKSRTA NPRASTTPSI DSTLDSSKLE STPELSMCST ADTSFSTFGS SLSMSHYNPF
QDGNDILQSP TVKARGNRKV KALPKRAGKQ QPDEVDNGKI PFLCLSVAFV
//
