ID B2AXN8_PODAN Unreviewed; 1450 AA.
AC B2AXN8;
DT 20-MAY-2008, integrated into UniProtKB/TrEMBL.
DT 20-MAY-2008, sequence version 1.
DT 24-JAN-2024, entry version 71.
DE RecName: Full=Pre-mRNA-splicing factor {ECO:0000256|PIRNR:PIRNR038901};
GN ORFNames=PODANS_7_11180 {ECO:0000313|EMBL:CAP69162.1};
OS Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383)
OS (Pleurage anserina).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Podosporaceae; Podospora;
OC Podospora anserina.
OX NCBI_TaxID=515849 {ECO:0000313|EMBL:CAP69162.1};
RN [1] {ECO:0000313|EMBL:CAP69162.1, ECO:0000313|Proteomes:UP000001197}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S / ATCC MYA-4624 / DSM 980 / FGSC 10383
RC {ECO:0000313|Proteomes:UP000001197}, and S mat+
RC {ECO:0000313|EMBL:CAP69162.1};
RX PubMed=18460219; DOI=10.1186/gb-2008-9-5-r77;
RA Espagne E., Lespinet O., Malagnac F., Da Silva C., Jaillon O., Porcel B.M.,
RA Couloux A., Aury J.-M., Segurens B., Poulain J., Anthouard V.,
RA Grossetete S., Khalili H., Coppin E., Dequard-Chablat M., Picard M.,
RA Contamine V., Arnaise S., Bourdais A., Berteaux-Lecellier V., Gautheret D.,
RA de Vries R.P., Battaglia E., Coutinho P.M., Danchin E.G.J., Henrissat B.,
RA El Khoury R., Sainsard-Chanet A., Boivin A., Pinan-Lucarre B., Sellem C.H.,
RA Debuchy R., Wincker P., Weissenbach J., Silar P.;
RT "The genome sequence of the model ascomycete fungus Podospora anserina.";
RL Genome Biol. 9:R77.1-R77.22(2008).
RN [2] {ECO:0000313|EMBL:CAP69162.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=S mat+ {ECO:0000313|EMBL:CAP69162.1};
RA Genoscope - CEA;
RL Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000001197}
RP GENOME REANNOTATION.
RC STRAIN=S / ATCC MYA-4624 / DSM 980 / FGSC 10383
RC {ECO:0000313|Proteomes:UP000001197};
RX PubMed=24558260; DOI=10.1534/genetics.113.159988;
RA Grognet P., Bidard F., Kuchly C., Tong L.C.H., Coppin E., Benkhali J.A.,
RA Couloux A., Wincker P., Debuchy R., Silar P.;
RT "Maintaining two mating types: Structure of the mating type locus and its
RT role in heterokaryosis in Podospora anserina.";
RL Genetics 197:421-432(2014).
RN [4] {ECO:0000313|EMBL:CDP32642.1}
RP NUCLEOTIDE SEQUENCE.
RA Grognet P., Bidard F., Kuchly C., Chan Ho Tong L., Coppin E.,
RA Ait Benkhali J., Couloux A., Wincker P., Debuchy R., Silar P.;
RT "Maintaining two mating types: Structure of the mating type locus and its
RT role in heterokaryosis in Podospora anserina.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in mRNA splicing where it associates with cdc5 and
CC the other cwf proteins as part of the spliceosome.
CC {ECO:0000256|PIRNR:PIRNR038901}.
CC -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome.
CC {ECO:0000256|PIRNR:PIRNR038901}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|PIRNR:PIRNR038901}.
CC -!- SIMILARITY: Belongs to the CWF11 family.
CC {ECO:0000256|PIRNR:PIRNR038901}.
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DR EMBL; CU633900; CAP69162.1; -; Genomic_DNA.
DR EMBL; FO904942; CDP32642.1; -; Genomic_DNA.
DR RefSeq; XP_001908489.1; XM_001908454.1.
DR STRING; 515849.B2AXN8; -.
DR GeneID; 6192353; -.
DR KEGG; pan:PODANSg5524; -.
DR VEuPathDB; FungiDB:PODANS_7_11180; -.
DR eggNOG; KOG1806; Eukaryota.
DR HOGENOM; CLU_001195_0_0_1; -.
DR InParanoid; B2AXN8; -.
DR OrthoDB; 7041at2759; -.
DR Proteomes; UP000001197; Chromosome 7.
DR GO; GO:0005684; C:U2-type spliceosomal complex; IEA:UniProtKB-UniRule.
DR GO; GO:0004386; F:helicase activity; IEA:InterPro.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IEA:UniProtKB-UniRule.
DR CDD; cd17935; EEXXQc_AQR; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR048966; Aquarius_b-barrel.
DR InterPro; IPR048967; Aquarius_insert.
DR InterPro; IPR032174; Aquarius_N.
DR InterPro; IPR026300; CWF11_fam.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR PANTHER; PTHR10887:SF5; RNA HELICASE AQUARIUS; 1.
DR Pfam; PF13086; AAA_11; 1.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF16399; Aquarius_N_1st; 1.
DR Pfam; PF21143; Aquarius_N_2nd; 1.
DR Pfam; PF21144; Aquarius_N_3rd; 1.
DR PIRSF; PIRSF038901; AQR_cwf11; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW mRNA processing {ECO:0000256|PIRNR:PIRNR038901};
KW mRNA splicing {ECO:0000256|PIRNR:PIRNR038901};
KW Nucleus {ECO:0000256|PIRNR:PIRNR038901};
KW Reference proteome {ECO:0000313|Proteomes:UP000001197}.
FT DOMAIN 33..418
FT /note="RNA helicase aquarius N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16399"
FT DOMAIN 496..660
FT /note="RNA helicase aquarius beta-barrel"
FT /evidence="ECO:0000259|Pfam:PF21143"
FT DOMAIN 708..800
FT /note="RNA helicase aquarius insertion"
FT /evidence="ECO:0000259|Pfam:PF21144"
FT DOMAIN 812..1108
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1119..1311
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 752..773
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1414..1450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 756..773
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1426..1450
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1450 AA; 163047 MW; 9C008D7E3BDB6150 CRC64;
MPPAKRVKSS ANSGSNAASG RPTVEDLEGQ SEFATLARQH WLPRKPAEVK VNNDVLKGEI
WDTLEKENFP LKSLLVLEGL QALESYLWPG YGEDSSNFHV LLIILIVNVK RRERLDTWDI
FSDRPADFSD LFRRALSLTV DSSLSWAIKT HVLLFIIHAF SSLDCTIVRK ECAPLVSISI
WHNISTDDKR DAILDSNTQW RKAWRASAKR YDAADDATKA RLRFERSWLY TSVLNFLNLL
YTDNAKPDQV LYCERFVEFL TDLQSQLPTR RYVNTLLQDL HVLPALVLSP VYNDEANGLL
RDLCRLFSHY TYFTIDEQTG AQLSKTEAYD RHCGDLAKLQ RTALKHFKDK LTVLALSNYG
AIDKRSELEG LLTVLADAEI ESLCKLLGLR TAYPDSVRVP IGRRFLLEVL LSQFERRKTF
QEVASELSLL PTEESLFEVG LGRTEHYDGS HPLALPKLNL QYLSVGDFLW RSMILYRCEA
FYAIRQDIES VLSRLKPEAK RSGETVFSGF SKMALKITKP TILEAVPPLV GDDKPSTVRA
EVTIDLRKLP QHVRREWESL RPDDVLFLLA VDASKPKLAT NGGASVTEAE KLGLVAVRAA
EVIQVLDDRG RAIRDAQAYF DGHNRSDIRR LQLRLDAKAF KDDTEGKQDV YEGINLLVRR
SGRENNFKPV LESIQDLTLS DVPLAPWLHE VFLGYGDPAG ANYKQLANRL RKVNFRDTFL
DWQHLVESLP GKIVEPDDDV SGSFGPPYVL ETVEKPAEEA PSKPSKKRRR DAEPALIAEI
ETLKVSTYKP PNNGPYPVDA PKLNSVRFTP TQIEAIISGT QPGLTVIVGP PGTGKTDVAT
QIINNIYHNF PEQKTLLIAH SNQALNQLFA KIVALDIDER HLLRLGHGEE ELETEGSFSK
HGRVESFLEN RQRFLYEVNR LAASIGAPGA HGSSAETAGY FNSVYIEPAW AKFNEIIKVE
DATPEDIVTV FPFHGYFADA PQPLFPPGAD RETVLEIANG CYRHISKIFS ELADVLPFEI
LRRDKDKANY LLTNEARIVA MTSTHAAMKR GEIAALGFQY DNVIMEEAAQ ITEIGNFIPL
ALQKPKDGQQ ALQRVVLCGD HYQNSPVIQG LAFRHYANLE QSLFSRLVRL GVPTINLDQQ
GRARPSISSL YKWRYPQLGD LPHTRTEKEF TTANAGFKYD YQFINVPDYK GEGETEPTPH
FIQNLGEAEY AVAIYQYMRL LGYPASKISI LATYAGQKAL IRDVLAHRCA KNPIFGLPRI
VTTVDKYQGE QNDYIILSLT RTSRVGYLRD IRRLTVALSR ARLGLYILGR REVFEACYEL
RDAFELLLKR PDQLTLVTGE LWPSERPLVV EQQEGGDGSA DNTAVATAVL GEAAMQGVEH
LGQYVYEMTK TKVEELKLLP QATVPSVDVE IRDVLEDTGL EDEGAAGAGG EEEEEEEEGR
KVEGFEAEED
//
