ID TUSA_YERPB Reviewed; 84 AA.
AC B2K098;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 27-MAR-2024, entry version 73.
DE RecName: Full=Sulfur carrier protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur mediator TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=Sulfur transfer protein TusA {ECO:0000255|HAMAP-Rule:MF_00413};
DE AltName: Full=tRNA 2-thiouridine synthesizing protein A {ECO:0000255|HAMAP-Rule:MF_00413};
GN Name=tusA {ECO:0000255|HAMAP-Rule:MF_00413}; OrderedLocusNames=YPTS_0229;
OS Yersinia pseudotuberculosis serotype IB (strain PB1/+).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=502801;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PB1/+;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C.,
RA Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.,
RA Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P.;
RT "Complete sequence of Yersinia pseudotuberculosis PB1/+.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sulfur carrier protein involved in sulfur trafficking in the
CC cell. Part of a sulfur-relay system required for 2-thiolation during
CC synthesis of 2-thiouridine of the modified wobble base 5-
CC methylaminomethyl-2-thiouridine (mnm(5)s(2)U) in tRNA. Interacts with
CC IscS and stimulates its cysteine desulfurase activity. Accepts an
CC activated sulfur from IscS, which is then transferred to TusD, and thus
CC determines the direction of sulfur flow from IscS to 2-thiouridine
CC formation. Also appears to be involved in sulfur transfer for the
CC biosynthesis of molybdopterin. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- PATHWAY: tRNA modification. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SUBUNIT: Interacts with IscS. {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00413}.
CC -!- SIMILARITY: Belongs to the sulfur carrier protein TusA family.
CC {ECO:0000255|HAMAP-Rule:MF_00413}.
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DR EMBL; CP001048; ACC87226.1; -; Genomic_DNA.
DR RefSeq; WP_002215973.1; NZ_CP009780.1.
DR AlphaFoldDB; B2K098; -.
DR SMR; B2K098; -.
DR GeneID; 66843381; -.
DR KEGG; ypb:YPTS_0229; -.
DR PATRIC; fig|502801.10.peg.3908; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0097163; F:sulfur carrier activity; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:InterPro.
DR CDD; cd03423; SirA; 1.
DR Gene3D; 3.30.110.40; TusA-like domain; 1.
DR HAMAP; MF_00413; Thiourid_synth_A; 1.
DR InterPro; IPR022931; Sulphur_carrier_TusA.
DR InterPro; IPR001455; TusA-like.
DR InterPro; IPR036868; TusA-like_sf.
DR PANTHER; PTHR33279:SF6; SULFUR CARRIER PROTEIN TUSA; 1.
DR PANTHER; PTHR33279; SULFUR CARRIER PROTEIN YEDF-RELATED; 1.
DR Pfam; PF01206; TusA; 1.
DR SUPFAM; SSF64307; SirA-like; 1.
DR PROSITE; PS01148; UPF0033; 1.
PE 3: Inferred from homology;
KW Cytoplasm; tRNA processing.
FT CHAIN 1..84
FT /note="Sulfur carrier protein TusA"
FT /id="PRO_1000199938"
FT ACT_SITE 19
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00413"
SQ SEQUENCE 84 AA; 9536 MW; D39211DAC090B7D5 CRC64;
MTDIFANPDK TLDALGLRCP EPVMMVRKTV RHMEEGQTLL IIADDPATTR DIPGFCRFMD
HQLLAQDTEQ TPYRYLVRKG ITAG
//
