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Database: UniProt
Entry: B2THC8
LinkDB: B2THC8
Original site: B2THC8 
ID   ADDA_CLOBB              Reviewed;        1243 AA.
AC   B2THC8;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-APR-2018, entry version 68.
DE   RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE   AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   Name=addA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   OrderedLocusNames=CLL_A0025;
OS   Clostridium botulinum (strain Eklund 17B / Type B).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=935198;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Eklund 17B / Type B;
RA   Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA   Smith T.J., Sutton G., Brettin T.S.;
RT   "Complete sequence of Clostridium botulinum strain Eklund.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA
CC       helicase and an ATP-dependent, dual-direction single-stranded
CC       exonuclease. Recognizes the chi site generating a DNA molecule
CC       suitable for the initiation of homologous recombination. The AddA
CC       nuclease domain is required for chi fragment generation; this
CC       subunit has the helicase and 3' -> 5' nuclease activities.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC       Rule:MF_01451}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
DR   EMBL; CP001056; ACD24582.1; -; Genomic_DNA.
DR   RefSeq; WP_012425324.1; NC_018648.1.
DR   SMR; B2THC8; -.
DR   PRIDE; B2THC8; -.
DR   EnsemblBacteria; ACD24582; ACD24582; CLL_A0025.
DR   GeneID; 19963759; -.
DR   KEGG; cbk:CLL_A0025; -.
DR   PATRIC; fig|935198.13.peg.16; -.
DR   HOGENOM; HOG000015621; -.
DR   KO; K16898; -.
DR   OMA; KQSIYRW; -.
DR   Proteomes; UP000001195; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006302; P:double-strand break repair; IEA:InterPro.
DR   Gene3D; 3.90.320.10; -; 1.
DR   HAMAP; MF_01451; AddA; 1.
DR   InterPro; IPR014152; DNA_helicase_suAddA.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR011604; Exonuc_phg/RecB_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   PANTHER; PTHR11070:SF14; PTHR11070:SF14; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 3.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding;
KW   Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1   1243       ATP-dependent helicase/nuclease subunit
FT                                A.
FT                                /FTId=PRO_0000379251.
FT   DOMAIN        4    477       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   DOMAIN      512    810       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   NP_BIND      25     32       ATP. {ECO:0000255|HAMAP-Rule:MF_01451}.
SQ   SEQUENCE   1243 AA;  144182 MW;  0E0C531AD31A3DF0 CRC64;
     MSGTKWTEEQ LSAITTRDCN LLVAAAAGSG KTAVLVERII KIITNEENPI DIDKLLVVTF
     TSAAAAEMRE RIANAISKKL DETPTSKNLQ KQLTLLNRSN IMTIHSFCLG VIKNNFHKID
     LDPSFRICDQ TEGILLKMEI IDELFDDKYD EENQEFIKFI EAFSSYKSDN ALKELVLSLY
     NFTMAGPWPK KWLKDASRDF DIKTLQELDE TKWVNVLKES IKIELDGYIK MMQKAVKLIN
     ETDGLEPYFE GFSSELDLIV NAYNNVESSL NDLYNSLNLI TFNRLKTIKK NTVSDENIQN
     LVKQIRDQVK KKISALIEGT FIASPDKMLD NIIKSYPYIN QLTELTSEFI DRFTAKKKEK
     NILDFNDLEH LCLKILIEEN EENQIVPSTI AQKFKDYFEE VLVDEYQDSN NVQEAIIELV
     SRKNSDNPNV FMVGDVKQSI YKFRQAKPEL FIDKYNSYSL DKGINRKIQL YKNFRSREEI
     INGVNYIFKS VMSKTVGELE YTDVEALNLG ANYPEKKNDD DIIGGPIEVH ILDRSDNKEE
     NDEFKLQAEE EIGDVNLEAR IIVKRINELI SKKEGNKFKV LDKDTGEYRD LKYKDIVILL
     RATKNWSEVL LDELGLAGIP VYADTGSGYF ESIEIRTIMS LLKVIDNPMQ DVPMLSLLIS
     PIIGLSAEEL SDIRLIDKEK YFYENIIKIS KEKLISVELQ EKCEYILSSI DKWRRKSIYM
     PIDEFIWYLY MDTAYYGYVG AMPNGVLRQA NLKILFQRAR QFSDTSFKGL FNFINFINKL
     TKSSGDMGSA KILGENEDVI RIMSIHKSKG LEFPVVFLAG CGKNFNLMDL NNKILYHEEL
     GLGPEYINLE NRTSITTLPK EAIKKRMKLE TLSEEMRVLY VAFTRAKEKL IITGAVRNAE
     KSIEKWINSA VLDKDVVLPY EISKGKSYLD WIGMALCKHK DGEILRKKLG FSSEMCKDDL
     SMWKISIWNK YELDMYDELD ENQEEEDVKI SILDKDVNKE IKSEVYRRLG YEYEFKESTK
     LTSNISVSDL KKRNIEDDID TLKMFDLEEE DNKNKDIITP KFLQEEKGIS AAERGTAIHF
     AMKKIDFSKV GTLKEIKEQL NKLYDEELIL QEEYNSINPY KILSFFKSSL GKKMLYVYNE
     GGKVYREVPF YTEISSLEID ESLPQKYANE KIRLQGIIDC FFKCDDEIIL LDYKTDYVEN
     EEEFKDKYKN QLSYYSEAIF KMTGKKVDKK YLYSFYLEKE ILI
//
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