ID B2WEW4_PYRTR Unreviewed; 1472 AA.
AC B2WEW4;
DT 01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT 01-JUL-2008, sequence version 1.
DT 27-MAR-2024, entry version 89.
DE RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN ORFNames=PTRG_08687 {ECO:0000313|EMBL:EDU51606.1};
OS Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus)
OS (Drechslera tritici-repentis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae;
OC Pyrenophora.
OX NCBI_TaxID=426418 {ECO:0000313|EMBL:EDU51606.1, ECO:0000313|Proteomes:UP000001471};
RN [1] {ECO:0000313|Proteomes:UP000001471}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Pt-1C-BFP {ECO:0000313|Proteomes:UP000001471};
RX PubMed=23316438; DOI=10.1534/g3.112.004044;
RA Manning V.A., Pandelova I., Dhillon B., Wilhelm L.J., Goodwin S.B.,
RA Berlin A.M., Figueroa M., Freitag M., Hane J.K., Henrissat B., Holman W.H.,
RA Kodira C.D., Martin J., Oliver R.P., Robbertse B., Schackwitz W.,
RA Schwartz D.C., Spatafora J.W., Turgeon B.G., Yandava C., Young S., Zhou S.,
RA Zeng Q., Grigoriev I.V., Ma L.-J., Ciuffetti L.M.;
RT "Comparative genomics of a plant-pathogenic fungus, Pyrenophora tritici-
RT repentis, reveals transduplication and the impact of repeat elements on
RT pathogenicity and population divergence.";
RL G3 (Bethesda) 3:41-63(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|RuleBase:RU000442};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU000442}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DS231623; EDU51606.1; -; Genomic_DNA.
DR RefSeq; XP_001939019.1; XM_001938984.1.
DR STRING; 426418.B2WEW4; -.
DR EnsemblFungi; EDU51606; EDU51606; PTRG_08687.
DR GeneID; 6346974; -.
DR eggNOG; KOG0970; Eukaryota.
DR HOGENOM; CLU_001718_1_0_1; -.
DR InParanoid; B2WEW4; -.
DR OMA; MTKMNVG; -.
DR OrthoDB; 211439at2759; -.
DR Proteomes; UP000001471; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IEA:InterPro.
DR CDD; cd05776; DNA_polB_alpha_exo; 1.
DR CDD; cd05532; POLBc_alpha; 1.
DR Gene3D; 2.40.50.730; -; 1.
DR Gene3D; 3.30.70.2820; -; 1.
DR Gene3D; 6.10.10.100; -; 1.
DR Gene3D; 1.10.3200.20; DNA Polymerase alpha, zinc finger; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR024647; DNA_pol_a_cat_su_N.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR038256; Pol_alpha_znc_sf.
DR InterPro; IPR045846; POLBc_alpha.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR015088; Znf_DNA-dir_DNA_pol_B_alpha.
DR NCBIfam; TIGR00592; pol2; 1.
DR PANTHER; PTHR45861; DNA POLYMERASE ALPHA CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR45861:SF1; DNA POLYMERASE ALPHA CATALYTIC SUBUNIT; 1.
DR Pfam; PF12254; DNA_pol_alpha_N; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF08996; zf-DNA_Pol; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|RuleBase:RU000442};
KW DNA-binding {ECO:0000256|RuleBase:RU000442};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU000442};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU000442};
KW Reference proteome {ECO:0000313|Proteomes:UP000001471};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000442};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 13..78
FT /note="DNA polymerase alpha catalytic subunit N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12254"
FT DOMAIN 427..720
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 785..1225
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 1264..1466
FT /note="Zinc finger DNA-directed DNA polymerase family B
FT alpha"
FT /evidence="ECO:0000259|Pfam:PF08996"
FT REGION 62..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 153..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 289..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..120
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 168..182
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 292..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1472 AA; 165167 MW; 783154C4592422BB CRC64;
MPRVAKKTGA ALLAELRANR AAGKTRLSTY EVAEEEQLYD TVDEEGYKKI VRGRLDEDDF
VVGDEGEGYV DDGREEWDDD QGKYHYAATD SEGEERPAKG KAAKRKREED EEKRKKIDKG
ISKYFNAKNA AKAPKAKPVA TEADKDFMND LLGEIDTNPA RSSSSRMRPI KTEARRKTRV
LSPPISENRP AATKRRTDDS DAYMPDTPPA LNAHADDDGL PTFDDDIAMS DPPLPSSPVT
KAVERRTQPV VKQEEEEDDL MEIAQPTTHH GVPVASVNMA GRRPIAKLKE ENPTPANSSP
VGPSTASFDA SSWSDVTSKL NVMSSPAPQN TGIGKMEPQD AVEEDGSLRF FWIDYTEING
SLCLFGKVKN NKTGNYSSCF VKVDNILRKL YFLPREYRHV RGVATDEEVG LSDVSDEVDS
IMSKHRVNLH KIKPCTRKYA FELPDIPKEA DYLKLLYGYD KMPLSSDLKG ETFSHVFGTN
TSLFEQFVLW KNIMGPCWLK IDNPDFGSLS NASWCKLEVL VSKPNNIAVM SASDNLDAPP
MTLMSLSLRT TFNAKDNKQE ILMASAMIYD NFSLTDTTPV DQMQCKSFTI MRPNGSSFPI
GFEKEVEKIK GNMYLKKTEQ ELLSLLMAMF QRHDPDVLIG HRLDDVDYSV LLSRLREKKT
PGWHRIGRLK RSDWPKSMGK GGGSFFAEKQ LASGRLLCDL ANDLGKSLMT KCQSWSLEEM
CQLVLNKRRE ELDNDAALKT WATTKEGLLN YVKHCQADAY FIAAISIKVQ MLPLTKVLTN
LAGNSWGRTL SGTRAERNEY ILLHEFHKNK YICPDKQWGK SKAKVDEEAA EGEEGADAKK
KDKFKGGLVF EPEKGLYDKF ILVMDFNSLY PSIIQEFNIC FTTVERSDLS EDDDKVPEVP
TDQQQGILPR LIATLVSRRR EVKKLMKAKD ATSDQLATWD IKQMALKLTA NSMYGCLGYT
KSRFYARPLA MLTTYKGREI LRKTKDMAEV KALRVIYGDT DSVMINTNVD NIQDALKLGN
EFKREVNDSY KLLEIDIDNV FRRILLHAKK KYAAINMVPV DGKYIEKLEV KGLDMRRREY
CALSKETSTE LLNFLLSGED PEVVVEKIHN HLRELSKQMR EYAIPARKYT IYTQLGKNPK
EYPNGGSMPA VQVALRLQSK GKLVKAKDVM SFIITGESSG SAENAAKNAF PVDEVLKPDS
GLKPDIDYYL HKQILPPVER LCAPISGTNI TLLAACLGLD SSKYRVSTVS GSAQQDTEIH
PLESQIPDSV RFQSCSPLWL RCRTCTTVFP FQGLASTSPT TLSLQTISHN GIQCPAPTCG
TIIPNITVVA QLESAIRQIL ATYYAGHVVC DDPQCSNRTR SISVYGHRCL GPKGLARDCL
GKMSYVVKER DVWNQLLYFA SLFDVDRVGK DVGGGSAGVK AEEMERREKI KVLAEVNRER
FGTLKGVVEK WLERNGRQWV QMDSLFSFAL KA
//
