ID   B4E9J5_BURCJ            Unreviewed;       534 AA.
AC   B4E9J5;
DT   23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT   23-SEP-2008, sequence version 1.
DT   27-MAR-2024, entry version 73.
DE   SubName: Full=Benzoylformate decarboxylase {ECO:0000313|EMBL:CAR53096.1};
GN   ORFNames=BCAL2796 {ECO:0000313|EMBL:CAR53096.1};
OS   Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 /
OS   NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=216591 {ECO:0000313|EMBL:CAR53096.1, ECO:0000313|Proteomes:UP000001035};
RN   [1] {ECO:0000313|EMBL:CAR53096.1, ECO:0000313|Proteomes:UP000001035}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 /
RC   CF5610 {ECO:0000313|Proteomes:UP000001035};
RX   PubMed=18931103; DOI=10.1128/JB.01230-08;
RA   Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D.,
RA   Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S.,
RA   Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K.,
RA   Scott P., Walker D., White B., Rose H., Iversen P., Mil-Homens D.,
RA   Rocha E.P., Fialho A.M., Baldwin A., Dowson C., Barrell B.G., Govan J.R.,
RA   Vandamme P., Hart C.A., Mahenthiralingam E., Parkhill J.;
RT   "The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of
RT   cystic fibrosis patients.";
RL   J. Bacteriol. 191:261-277(2009).
CC   -!- SIMILARITY: Belongs to the TPP enzyme family.
CC       {ECO:0000256|ARBA:ARBA00007812, ECO:0000256|RuleBase:RU362132}.
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DR   EMBL; AM747720; CAR53096.1; -; Genomic_DNA.
DR   RefSeq; WP_012492843.1; NC_011000.1.
DR   AlphaFoldDB; B4E9J5; -.
DR   KEGG; bcj:BCAL2796; -.
DR   eggNOG; COG0028; Bacteria.
DR   HOGENOM; CLU_013748_3_1_4; -.
DR   BioCyc; BCEN216591:G1G1V-3097-MONOMER; -.
DR   Proteomes; UP000001035; Chromosome 1.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR   CDD; cd02002; TPP_BFDC; 1.
DR   CDD; cd07035; TPP_PYR_POX_like; 1.
DR   Gene3D; 3.40.50.970; -; 2.
DR   Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR   InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR   InterPro; IPR000399; TPP-bd_CS.
DR   InterPro; IPR045229; TPP_enz.
DR   InterPro; IPR011766; TPP_enzyme_TPP-bd.
DR   PANTHER; PTHR18968:SF133; BENZOYLFORMATE DECARBOXYLASE; 1.
DR   PANTHER; PTHR18968; THIAMINE PYROPHOSPHATE ENZYMES; 1.
DR   Pfam; PF02775; TPP_enzyme_C; 1.
DR   Pfam; PF00205; TPP_enzyme_M; 1.
DR   Pfam; PF02776; TPP_enzyme_N; 1.
DR   SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR   SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR   PROSITE; PS00187; TPP_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Thiamine pyrophosphate {ECO:0000256|RuleBase:RU362132}.
FT   DOMAIN          12..113
FT                   /note="Thiamine pyrophosphate enzyme N-terminal TPP-
FT                   binding"
FT                   /evidence="ECO:0000259|Pfam:PF02776"
FT   DOMAIN          197..327
FT                   /note="Thiamine pyrophosphate enzyme central"
FT                   /evidence="ECO:0000259|Pfam:PF00205"
FT   DOMAIN          390..531
FT                   /note="Thiamine pyrophosphate enzyme TPP-binding"
FT                   /evidence="ECO:0000259|Pfam:PF02775"
SQ   SEQUENCE   534 AA;  56883 MW;  375A781A0C7DC544 CRC64;
     MSGHPPPSAS ITVRDAVVDL LRQFGIDRVF GNPGSTELPM FRDFPDDFRY VLGLHEAVVV
     GMADGHAQAT GNAAVVNLHS AAGVGNAMGN LFTAFKNRTP LIVTAGQQAR AILPFDPFLG
     ATQAAELPKP YVKWSIEPAR AQDVPAAIAR AYRIAMQEPR GPVFVSIPVD DWDQPAERLP
     RRDVSSIVRP DPDALARLGD ALDAARRPAF VVGAAVDRAG AWDDVVRLAE RHRARVYVAP
     MSGRCSFPED HPLFAGFLPA IREKIIARLD GHDLVFAFGA PAFTYHIEGF GPHVPPGATL
     VQLVDDPGVA AWTPSGDAVV GNLRLAARDL LARPAPPERP MPAARAPRVR VDAPAAGERM
     SVAFALQTLA DVRDASDIVV EEAPSARPVM QEHLPFTRSG TFYTMDSGGL GYGMPAAVGV
     ALAQPGRRVI GLIGDGSSLY SIQALWSAAQ LKLPITFVIL NNRRYAALQD FAPVFGFGPG
     DPVQGTELPD LDFVALAQGM GCRGVRVAEA AKLRDTLTDA LRAATPVVVE VEIA
//