ID B4ENF7_BURCJ Unreviewed; 565 AA.
AC B4ENF7;
DT 23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT 23-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 75.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN Name=zmpA {ECO:0000313|EMBL:CAR57339.1};
GN ORFNames=BCAS0409 {ECO:0000313|EMBL:CAR57339.1};
OS Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 /
OS NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=216591 {ECO:0000313|EMBL:CAR57339.1, ECO:0000313|Proteomes:UP000001035};
RN [1] {ECO:0000313|EMBL:CAR57339.1, ECO:0000313|Proteomes:UP000001035}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 /
RC CF5610 {ECO:0000313|Proteomes:UP000001035};
RX PubMed=18931103; DOI=10.1128/JB.01230-08;
RA Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D.,
RA Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S.,
RA Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K.,
RA Scott P., Walker D., White B., Rose H., Iversen P., Mil-Homens D.,
RA Rocha E.P., Fialho A.M., Baldwin A., Dowson C., Barrell B.G., Govan J.R.,
RA Vandamme P., Hart C.A., Mahenthiralingam E., Parkhill J.;
RT "The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of
RT cystic fibrosis patients.";
RL J. Bacteriol. 191:261-277(2009).
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
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DR EMBL; AM747722; CAR57339.1; -; Genomic_DNA.
DR RefSeq; WP_006481898.1; NC_011002.1.
DR AlphaFoldDB; B4ENF7; -.
DR SMR; B4ENF7; -.
DR MEROPS; M04.022; -.
DR KEGG; bcj:BCAS0409; -.
DR eggNOG; COG3227; Bacteria.
DR HOGENOM; CLU_008590_5_1_4; -.
DR BioCyc; BCEN216591:G1G1V-7406-MONOMER; -.
DR Proteomes; UP000001035; Chromosome 3.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF1; BACILLOLYSIN; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 23..565
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5023036220"
FT DOMAIN 70..116
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 222..384
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 387..564
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT ACT_SITE 377
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 465
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 565 AA; 59904 MW; 95A43F82A6EC351B CRC64;
MKKLSRLLPI TAITLASLSA FAQAGDQPAA VDKALQLIQQ NPSAFSLAAG GTARTLKFAG
PQAGAPTDGD QFQVRDVIVD PDGTEHVRFD RFHAGLPVIG GDVVVHSSKG QLKQASVTQL
APINLAGSIG KVGNRAVIRN APDVGATRAR HIAAARFNSD VRRVDDAELV VFARDVTPTL
AYAVRVYGKA TETHGDAVLY YVDARTGTLL DAQDLIKTAA ATGTGRSLYY GNLTLTTDQT
GTNAYRMLDP SRGGGSVYDG RGLSSDEVEQ ATDLPIFTSS TNVWGNNATT DRQTVAADID
YGLALTWDYY KTTHNRNGIF NDGRGVKSYA HVVFNTGSGT TGANAAWLSS RVMAYGDGEP
GTRLPKPVVS VDVAGHEMSH GVTEATANLN YSGDAGGLNE STSDIFGTLV KFYANNPNDP
GNYVIGARVV SGGLRKMYKQ DLDGRSFSCY PSGGFSWSNP RHDPHFTSGV GNRFFYLLSE
GPSVPPTDTG LSRTQLVCNG DTTFSGLGRD KAGKIWYRTL TVYLNANSSY PNARRASIQA
ANDLYGANSA ESATVARAWS AVGVN
//
