ID B4U7G7_HYDS0 Unreviewed; 339 AA.
AC B4U7G7;
DT 23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT 23-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 73.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:ACG57078.1};
GN ORFNames=HY04AAS1_0388 {ECO:0000313|EMBL:ACG57078.1};
OS Hydrogenobaculum sp. (strain Y04AAS1).
OC Bacteria; Aquificota; Aquificae; Aquificales; Aquificaceae;
OC Hydrogenobaculum.
OX NCBI_TaxID=380749 {ECO:0000313|EMBL:ACG57078.1};
RN [1] {ECO:0000313|EMBL:ACG57078.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Y04AAS1 {ECO:0000313|EMBL:ACG57078.1};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Chertkov O., Kiss H., Brettin T.,
RA Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Mikhailova N., Resenback A.-L., Mead D.;
RL Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ACG57078.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Y04AAS1 {ECO:0000313|EMBL:ACG57078.1};
RX PubMed=19136599; DOI=10.1128/JB.01645-08;
RA Reysenbach A.L., Hamamura N., Podar M., Griffiths E., Ferreira S.,
RA Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A.,
RA Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A.;
RT "Complete and draft genome sequences of six members of the Aquificales.";
RL J. Bacteriol. 191:1992-1993(2009).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|PIRSR:PIRSR004869-50};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|PIRSR:PIRSR004869-50};
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DR EMBL; CP001130; ACG57078.1; -; Genomic_DNA.
DR AlphaFoldDB; B4U7G7; -.
DR STRING; 380749.HY04AAS1_0388; -.
DR KEGG; hya:HY04AAS1_0388; -.
DR eggNOG; COG1180; Bacteria.
DR HOGENOM; CLU_044176_1_1_0; -.
DR OrthoDB; 9778883at2; -.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR027596; AmmeMemoSam_rS.
DR InterPro; IPR034457; Organic_radical-activating.
DR InterPro; IPR016431; Pyrv-formate_lyase-activ_prd.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04337; AmmeMemoSam_rS; 1.
DR PANTHER; PTHR30352; PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME; 1.
DR PANTHER; PTHR30352:SF5; TRNA 4-DEMETHYLWYOSINE SYNTHASE (ADOMET-DEPENDENT); 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR PIRSF; PIRSF004869; PflX_prd; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01101; Uncharacterised_Radical_SAM_Su; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR004869-50};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR004869-
KW 50};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR004869-50};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PIRSR:PIRSR004869-50}.
FT DOMAIN 68..292
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT BINDING 83
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
FT BINDING 87
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
FT BINDING 90
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
SQ SEQUENCE 339 AA; 38736 MW; 36904CA96C2F00E5 CRC64;
MFIEASYWKT TEDGRVICEL CPDYCKLKEG QVGVCGVRLN KGGKLLSATY GSLVSIALDP
VEKKPLFHFL PGHKTLTIAT PGCNLHCLGC QNYEISQVKI DDSNKAFFEN SYISPEQIVQ
RAIETGSKSI SYSYSDPIIF YEYMFDIAKL AKEKGLKNIM VTAGYITEKP ALKLMDVIDA
FSIDLKFFSE KAYSKYSKGK LEPILNFIKL CLKHEKWIEL TTLLVPKYLD EEQLRLIAKF
ISKELGPHIP WHISRFFPYY KALDLHPTPE SMIKTAYEIG KEEGIYYVYT GNIKSEHEHT
VCPNCNNIVI ERDRFVIKSL KLEKGKCSFC GYKIEGVFE
//
