ID B4WRL4_SYNS7 Unreviewed; 1177 AA.
AC B4WRL4;
DT 23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT 23-SEP-2008, sequence version 1.
DT 27-MAR-2024, entry version 82.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=S7335_2553 {ECO:0000313|EMBL:EDX84855.1};
OS Synechococcus sp. (strain ATCC 29403 / PCC 7335).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales; Synechococcaceae;
OC Synechococcus.
OX NCBI_TaxID=91464 {ECO:0000313|EMBL:EDX84855.1, ECO:0000313|Proteomes:UP000005766};
RN [1] {ECO:0000313|EMBL:EDX84855.1, ECO:0000313|Proteomes:UP000005766}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29403 / PCC 7335 {ECO:0000313|Proteomes:UP000005766};
RA Tandeau de Marsac N., Ferriera S., Johnson J., Kravitz S., Beeson K.,
RA Sutton G., Rogers Y.-H., Friedman R., Frazier M., Venter J.C.;
RL Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; DS989904; EDX84855.1; -; Genomic_DNA.
DR RefSeq; WP_006454601.1; NZ_DS989904.1.
DR AlphaFoldDB; B4WRL4; -.
DR STRING; 91464.S7335_2553; -.
DR eggNOG; COG1197; Bacteria.
DR HOGENOM; CLU_005122_1_3_3; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000005766; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 3.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000005766}.
FT DOMAIN 643..804
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 829..981
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1177 AA; 132119 MW; C2B67B63B468336E CRC64;
MAFSSITRAI ARSPLAQELV AKLEKQQTLT ITGAPRLPKG LVSSALAQTQ NKPMLVVTAT
LEEAGRWSAQ LEAMGWATVA FYPTSESSPY DVFDPESELI WGQLQVLSDL INGKANPENM
PLGSKHLGTM AIVTTERALQ PHLPPVEAFR PYCLEITLGM ELNLKDLGLS LAKLGYEKVS
SVEVEGQWSQ RGDIIDVFPV AAELPVRMEL FGDELERLRE FDPANQRSLD KIEALLLTTT
DYGPLILSAL EEKELLEDLL TEESQETFAA TGKIEGARRF MGLAFESPAS ILDYLPDDTL
VVIDEPAQCL AHAERWIETV EDRWQEALDA FEADVEIQLP SGGLPKVHRP FSQALEEIEL
FQRIEISELA EEGVGLNLAS RPVPILPHQF GKLADILKEE RDRKFFPWLI SAQPSRSVAL
LQEHDCPAQF IPNPKDFPAI DKLQNQRIPI AVKYSGLAEL EGFVLPTFRI VVVTDREFFG
QHTLATPSYV RKRRRAASKQ VDPNKLRPKD YIVHRNHGIG QFLKLESLTV DKETREYLVI
QYADGLLRVP VDQMGSLSRY RTSVEQRPQL NKMTGKAWEK TKGKARKAIK KVAVDLLKLY
AQRSQMQGFT YPEDMPWQQE LEDSFPYNPT PDQLKATQDV KRDMESDRPM DRLVCGDVGF
GKTEVALRAV FKAVTAGKQV ALLAPTTILT QQHYHTLKER FAPYPIQIGL LNRFRTAKER
KDILLRLISG ELDVVVGTHQ LLGKTVKFKD LGLLVVDEEQ RFGVNQKEKI KAMKAQVDVL
TLSATPIPRT LYMALSGVRE MSLITTPPPS RRPIKTHLSP YDPEKVRTAI RQELDRGGQI
FYVVPRVEGI EEVAGRIREA VPGVRLAIAH GQMPEGELEA TMLTFSNGDA DLMVCTTIIE
SGLDIPRVNT IIIEDSQKFG LSQLYQLRGR VGRSGIQAHA WLLFPKQNQL SDKARKRLRA
IQEFTQLGSG YQLAMRDMEI RGIGNLLGAQ QSGQMEVIGF DLYMDMLEES IAEIRGQEIP
QVDETQVDLK VTAFIPADYI PELDQKMSVY RSLVGASTRR ELIEIVADLN DHYGSLPSAV
GQLVKVLELK QIAKPLGFSR IRTEDKQHVV LETPMEKPAW ALLHEKVPAH LRSRFVYAPG
KVTVRGLGAV KPEKQIENLI EWLGHMQTAL PEPAFSR
//
