ID B5CLK0_9FIRM Unreviewed; 386 AA.
AC B5CLK0;
DT 14-OCT-2008, integrated into UniProtKB/TrEMBL.
DT 14-OCT-2008, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Transposase, IS605 OrfB family {ECO:0000313|EMBL:EDY33852.1};
GN ORFNames=RUMLAC_00322 {ECO:0000313|EMBL:EDY33852.1};
OS [Ruminococcus] lactaris ATCC 29176.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Mediterraneibacter.
OX NCBI_TaxID=471875 {ECO:0000313|EMBL:EDY33852.1, ECO:0000313|Proteomes:UP000003254};
RN [1] {ECO:0000313|EMBL:EDY33852.1, ECO:0000313|Proteomes:UP000003254}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29176 {ECO:0000313|EMBL:EDY33852.1,
RC ECO:0000313|Proteomes:UP000003254};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Ruminococcus lactaris ATCC 29176.";
RL Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EDY33852.1, ECO:0000313|Proteomes:UP000003254}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29176 {ECO:0000313|EMBL:EDY33852.1,
RC ECO:0000313|Proteomes:UP000003254};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC family. {ECO:0000256|ARBA:ARBA00008761}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the transposase 2
CC family. {ECO:0000256|ARBA:ARBA00011044}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDY33852.1}.
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DR EMBL; ABOU02000014; EDY33852.1; -; Genomic_DNA.
DR RefSeq; WP_005609868.1; NZ_DS990179.1.
DR AlphaFoldDB; B5CLK0; -.
DR GeneID; 77334902; -.
DR eggNOG; COG0675; Bacteria.
DR HOGENOM; CLU_032903_0_2_9; -.
DR Proteomes; UP000003254; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR InterPro; IPR010095; Cas12f1-like_TNB.
DR InterPro; IPR001959; Transposase.
DR InterPro; IPR021027; Transposase_put_HTH.
DR NCBIfam; NF040570; guided_TnpB; 1.
DR NCBIfam; TIGR01766; IS200/IS605 family accessory protein TnpB-like domain; 1.
DR PANTHER; PTHR30405:SF20; RNA-GUIDED DNA ENDONUCLEASE INSQ-RELATED; 1.
DR PANTHER; PTHR30405; TRANSPOSASE; 1.
DR Pfam; PF12323; HTH_OrfB_IS605; 1.
DR Pfam; PF01385; OrfB_IS605; 1.
DR Pfam; PF07282; OrfB_Zn_ribbon; 1.
PE 3: Inferred from homology;
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Reference proteome {ECO:0000313|Proteomes:UP000003254};
KW Transposition {ECO:0000256|ARBA:ARBA00022578};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 1..49
FT /note="Transposase putative helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12323"
FT DOMAIN 173..300
FT /note="Probable transposase IS891/IS1136/IS1341"
FT /evidence="ECO:0000259|Pfam:PF01385"
FT DOMAIN 311..378
FT /note="Cas12f1-like TNB"
FT /evidence="ECO:0000259|Pfam:PF07282"
SQ SEQUENCE 386 AA; 44795 MW; 36F0CC241386AD26 CRC64;
MRKLLKSFKT EINPTVEQKI KINKTIGTCR YVYNFYLDHN KALYDKGEKF MTGKSFSVWL
NNEYIPDNPE KVWIKEAYSK AVKKSIEDGC TAFTRFFKHQ SAFPNFKKKG KSDVKMYFVK
NNPKDCRCER HRLNIPTLGW VRIKEKGYIP TTKDGWNIKS GTVSIKAGRY YVSVLVEIPD
AKIANNSNDG IGIDLGLKDL AIVSNGKTYK NINKSARVKK LEKKLRREQR CLSRKYENLK
KGESTQKNIQ KQKLKVQRLH HKIDNIRTDY INKSIAKIVK TKPSYITIED LNISGMMKNR
HLSKAVASQK FYEFRTKLKA KCDENGIELR VVDRWYPSSK ICHCCGAIKK DLKLSDRIYC
CDCGYVEDRD FNAALNLRDA LTYEVA
//
