ID   B5GHL9_STRSH            Unreviewed;       428 AA.
AC   B5GHL9;
DT   14-OCT-2008, integrated into UniProtKB/TrEMBL.
DT   13-JUL-2010, sequence version 2.
DT   27-MAR-2024, entry version 46.
DE   SubName: Full=Hydrolase {ECO:0000313|EMBL:EDY45815.3};
GN   ORFNames=SSBG_03808 {ECO:0000313|EMBL:EDY45815.3};
OS   Streptomyces sp. (strain SPB074).
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=465543 {ECO:0000313|EMBL:EDY45815.3, ECO:0000313|Proteomes:UP000002779};
RN   [1] {ECO:0000313|Proteomes:UP000002779}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SPB074 {ECO:0000313|Proteomes:UP000002779};
RG   The Broad Institute Genome Sequencing Platform;
RA   Fischbach M., Ward D., Young S., Jaffe D., Gnerre S., Berlin A., Heiman D.,
RA   Hepburn T., Sykes S., Alvarado L., Kodira C.D., Straight P., Clardy J.,
RA   Hung D., Kolter R., Mekalanos J., Walker S., Walsh C.T., Lander E.,
RA   Galagan J., Nusbaum C., Birren B.;
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:EDY45815.3, ECO:0000313|Proteomes:UP000002779}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SPB074 {ECO:0000313|Proteomes:UP000002779};
RG   The Broad Institute Genome Sequencing Platform;
RG   Broad Institute Microbial Sequencing Center;
RA   Fischbach M., Godfrey P., Ward D., Young S., Zeng Q., Koehrsen M.,
RA   Alvarado L., Berlin A.M., Bochicchio J., Borenstein D., Chapman S.B.,
RA   Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A.,
RA   Gujja S., Heilman E.R., Heiman D.I., Hepburn T.A., Howarth C., Jen D.,
RA   Larson L., Lewis B., Mehta T., Park D., Pearson M., Richards J.,
RA   Roberts A., Saif S., Shea T.D., Shenoy N., Sisk P., Stolte C., Sykes S.N.,
RA   Thomson T., Walk T., White J., Yandava C., Straight P., Clardy J., Hung D.,
RA   Kolter R., Mekalanos J., Walker S., Walsh C.T., Wieland-Brown L.C.,
RA   Haas B., Nusbaum C., Birren B.;
RT   "The genome sequence of Streptomyces sp. strain SPB74.";
RL   Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR   EMBL; GG770539; EDY45815.3; -; Genomic_DNA.
DR   AlphaFoldDB; B5GHL9; -.
DR   Proteomes; UP000002779; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd18820; GH43_LbAraf43-like; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR43817; GLYCOSYL HYDROLASE; 1.
DR   PANTHER; PTHR43817:SF1; HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|RuleBase:RU361187, ECO:0000313|EMBL:EDY45815.3}.
FT   SITE            109
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   428 AA;  46864 MW;  CFF10545F63E4775 CRC64;
     MTHWNGNYYL TTTQGNSVKM WRSPSLATLL TADPVTVWTD TDASRNQHIW APEFYRFGDR
     WYLYYTADDG TDDHHRVFVA ESDRDDPTGP YHFKAKLAPP NHANDFAIDP GILQVNGRLY
     LAYSGINQYQ HNGLNIAPLS NPYTVSGNAV ALNAAGGCPE VREGPEFLYR NGRTWMTYST
     CDTGKPDYQL WMMSLPDGAD PLVPGTWQQH QGPVFSRADD RGVYGPGHHA LFESPDGKED
     WIVYHAKSTS VNTYSNRTTR AQRIGWKADG SPDFGVPLAT GATQDLPSGD PGPGAYWIND
     DGRSSGAGSL AYTGTWNSGT GCGAQCFWGD DHWSATAGDT ATYTFTGTRI ALLSVRDTGN
     GYAAVSIDGG AEQRLDYYGT PRTGENLQYL SPRLPSGKHT LRVRVTGDRD GQSGAAFVGV
     DRAEVWTI
//