ID B6A3E3_RHILW Unreviewed; 475 AA.
AC B6A3E3;
DT 25-NOV-2008, integrated into UniProtKB/TrEMBL.
DT 25-NOV-2008, sequence version 1.
DT 27-MAR-2024, entry version 99.
DE RecName: Full=Aspartate ammonia-lyase {ECO:0000256|ARBA:ARBA00016146, ECO:0000256|RuleBase:RU362017};
DE Short=Aspartase {ECO:0000256|RuleBase:RU362017};
DE EC=4.3.1.1 {ECO:0000256|ARBA:ARBA00012992, ECO:0000256|RuleBase:RU362017};
GN OrderedLocusNames=Rleg2_5752 {ECO:0000313|EMBL:ACI58926.1};
OS Rhizobium leguminosarum bv. trifolii (strain WSM2304).
OG Plasmid pRLG202 {ECO:0000313|EMBL:ACI58926.1,
OG ECO:0000313|Proteomes:UP000008330}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=395492 {ECO:0000313|EMBL:ACI58926.1, ECO:0000313|Proteomes:UP000008330};
RN [1] {ECO:0000313|EMBL:ACI58926.1, ECO:0000313|Proteomes:UP000008330}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WSM2304 {ECO:0000313|EMBL:ACI58926.1,
RC ECO:0000313|Proteomes:UP000008330};
RC PLASMID=pRLG202 {ECO:0000313|EMBL:ACI58926.1};
RX PubMed=21304679; DOI=10.4056/sigs.44642;
RA Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K., Tiwari R.,
RA Malfatti S., Kiss H., Lapidus A., Copeland A., Nolan M., Land M.,
RA Ivanova N., Mavromatis K., Markowitz V., Kyrpides N., Melino V., Denton M.,
RA Yates R., Howieson J.;
RT "Complete genome sequence of Rhizobium leguminosarum bv trifolii strain
RT WSM2304, an effective microsymbiont of the South American clover Trifolium
RT polymorphum.";
RL Stand. Genomic Sci. 2:66-76(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-aspartate = fumarate + NH4(+); Xref=Rhea:RHEA:16601,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29806, ChEBI:CHEBI:29991; EC=4.3.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001494,
CC ECO:0000256|RuleBase:RU362017};
CC -!- SIMILARITY: Belongs to the class-II fumarase/aspartase family.
CC Aspartase subfamily. {ECO:0000256|ARBA:ARBA00005596,
CC ECO:0000256|RuleBase:RU362017}.
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DR EMBL; CP001193; ACI58926.1; -; Genomic_DNA.
DR RefSeq; WP_012555126.1; NC_011366.1.
DR AlphaFoldDB; B6A3E3; -.
DR KEGG; rlt:Rleg2_5752; -.
DR HOGENOM; CLU_021594_4_1_5; -.
DR Proteomes; UP000008330; Plasmid pRLG202.
DR GO; GO:0008797; F:aspartate ammonia-lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0006531; P:aspartate metabolic process; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR CDD; cd01357; Aspartase; 1.
DR Gene3D; 1.10.40.30; Fumarase/aspartase (C-terminal domain); 1.
DR Gene3D; 1.20.200.10; Fumarase/aspartase (Central domain); 1.
DR Gene3D; 1.10.275.10; Fumarase/aspartase (N-terminal domain); 1.
DR InterPro; IPR004708; ApsA.
DR InterPro; IPR024083; Fumarase/histidase_N.
DR InterPro; IPR018951; Fumarase_C_C.
DR InterPro; IPR020557; Fumarate_lyase_CS.
DR InterPro; IPR000362; Fumarate_lyase_fam.
DR InterPro; IPR022761; Fumarate_lyase_N.
DR InterPro; IPR008948; L-Aspartase-like.
DR NCBIfam; TIGR00839; aspA; 1.
DR PANTHER; PTHR42696; ASPARTATE AMMONIA-LYASE; 1.
DR PANTHER; PTHR42696:SF2; ASPARTATE AMMONIA-LYASE; 1.
DR Pfam; PF10415; FumaraseC_C; 1.
DR Pfam; PF00206; Lyase_1; 1.
DR PRINTS; PR00149; FUMRATELYASE.
DR SUPFAM; SSF48557; L-aspartase-like; 1.
DR PROSITE; PS00163; FUMARATE_LYASES; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|RuleBase:RU362017, ECO:0000313|EMBL:ACI58926.1};
KW Plasmid {ECO:0000313|EMBL:ACI58926.1}.
FT DOMAIN 18..348
FT /note="Fumarate lyase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00206"
FT DOMAIN 414..467
FT /note="Fumarase C C-terminal"
FT /evidence="ECO:0000259|Pfam:PF10415"
SQ SEQUENCE 475 AA; 51468 MW; 50A212174F97C05A CRC64;
MTKSEITTIR VEHDLIGDRD VPATAYYGVH TLRAVENFPI TGQTLKESAD LIASLAAIKQ
AAAQANASLG LLDQERADAI IAACVEIRDG ALHDHFVVDL IQGGAGTSTN MNANEVIANR
ALEIMGYGRG EYQHLHPNEH VNLSQSTNDV YPTALKLAAW IGVHRLVDAM AILRRAFEAK
AVEFADVLKM GRTQLQDAVP MTLGQEFGTY ALMLAEDEAR LSEAVSLIRE INLGATAIGT
GITAHPRYAA LVRERLSEIV GVDLVTSPDL VEATQDCGSF VQLSGVLKRV AVKLSKTCND
LRLLSSGPRA GFNEINLPAR QAGSSIMPGK VNPVIPEVVN QVAFEVIGND VTITMAAEAG
QLQLNAFEPV IFYSLYRSLS HLTNACLTLE ANCIQGITAN RDRLRQTVEQ SIGIVTALNP
YIGYRNATEV ALEAHHSGRG VYEILLERGL MQKEHLDAVL RPETLTRPSE KLSFS
//
