ID B6AD14_CRYMR Unreviewed; 3645 AA.
AC B6AD14;
DT 25-NOV-2008, integrated into UniProtKB/TrEMBL.
DT 25-NOV-2008, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN ORFNames=CMU_017720 {ECO:0000313|EMBL:EEA06018.1};
OS Cryptosporidium muris (strain RN66).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Cryptosporidiidae; Cryptosporidium.
OX NCBI_TaxID=441375 {ECO:0000313|Proteomes:UP000001460};
RN [1] {ECO:0000313|EMBL:EEA06018.1, ECO:0000313|Proteomes:UP000001460}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RN66 {ECO:0000313|EMBL:EEA06018.1,
RC ECO:0000313|Proteomes:UP000001460};
RA Lorenzi H., Inman J., Miller J., Schobel S., Amedeo P., Caler E.V.,
RA da Silva J.;
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC {ECO:0000256|ARBA:ARBA00010769}.
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DR EMBL; DS989728; EEA06018.1; -; Genomic_DNA.
DR RefSeq; XP_002140367.1; XM_002140331.1.
DR STRING; 441375.B6AD14; -.
DR EnsemblProtists; EEA06018; EEA06018; CMU_017720.
DR GeneID; 6995283; -.
DR VEuPathDB; CryptoDB:CMU_017720; -.
DR eggNOG; KOG0890; Eukaryota.
DR OMA; SLFCARS; -.
DR OrthoDB; 8448at2759; -.
DR Proteomes; UP000001460; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00892; PIKKc_ATR; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF72; SERINE-PROTEIN KINASE ATM; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:EEA06018.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000001460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EEA06018.1}.
FT DOMAIN 3265..3580
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 3613..3645
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
FT REGION 2091..2115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2091..2110
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3645 AA; 420538 MW; A39D4F8E36001CFE CRC64;
MNLKNSNELT EVISTLSIAE EDPRKLDEFV DLHCECIGTL RKYLLEGIKQ YIFGDQDVEK
SIITFSAMQS ALRGICCSYG HVIFPINGLG QLFYRLLSHF FAFRVQFKLV YFSDCEFIGQ
DYTSEYFINE MMLNKNYTSL VLQELVLLKK WSSSLCIIIK LISSEDGKIF ELFTNETIQN
IYKLAKLINE SNCSTQKTYD CSQIQDLYSH LLNHLRSYIG WPTGLQSPIA IRVPNDTLKV
LVLLGSLSIQ ILSILYTEEA ESHTRYCFIE AWICILKNVM VLYSNYELIA VSMHILENLT
LNYKLWPYTG NIMLLNLDIK LIEELYASSI FDSLSINNMW LPSFEKKTLV PFLHLIGEFT
VWALNTLKWQ FPLKLDISLI INSEYNKTPS LKVVKVLLKI IEKWQTLFLW SEFSLLDGYV
ALLTSRLYST ISTELSDEIS NIIFTKIRKD LLPVLDNSNI NNKYLPSRLK IRALENFLIK
LDIPKLTMDF EFCEADTLVQ TLGTTVCGLH EMYTSNISNH RIYFFIAHIQ IVLSKYFDCE
ALLVTPPVIY TKYLIIHLTL EMARLLIKKG TKNSFELGLY LMNHFCRLSE TVINLNNLSN
WTLSQLISLV EISIRLITVP QKVSKQMLLS FIDIVSIKCT FTSFIIWNYI SNVLYLYCIE
SEELDNDVTD KLLLKIGHLS ADERLSQAAL FNVLKPLSIL LLADLNHNTK DMIIQELNMT
RTTSVKNTFE AEYPFGSNYD SLSVFHYQEA ESDYPNTNYF PVEPNCWNCK EQIDMMDTIL
NKDEIYYCSK CLSPLPTSID IGQVDDSNNL DNYWTIQKLA LYYKKTGSTN NEHSSFLLTK
ILELIDNVPY TSSEWFFSSI ASILSLSSVN CNISMKIKVA LLIQASLSHN LKIPKLYQIQ
LLRLLNSPTF VLNFLTNESE TFNNRFLASY TKMPPPITII AAIDELRDPI TLFYRIIFEK
FKLSIIFGPT IGLYTTKHEI NDLEVIIHPI FGPIFALDEL IIYISSNNIN SQKVYMKFMN
LSRSMIYPNL LSQIQIDPPF SSKITHLTKN SKYVVPQWIY KELITNISDS FAQLHISNLV
AIYLNNLQNI KILWMETWIL KSYKEYSIGF LPINGKNIQD SKKSTNYSYQ AFVLLSYLSS
ILMNNKTTIS SLAISPGFNS CLAATFMDIF CTEGYNKYWS LLLLGSSYDY LLPHLLYTAL
PLFVQKPLLP SIIEWCHFMP NIFSYDKLIA QLAPFIISHL NTYNVPYTIL CNLSANNINF
FEYLISTYYS QVLQKSSDEI LFFPQSLICI CTIALLWGIT CPHHYFWILY DTEENSTGFN
QILKFFIEDE GIAFRIEDNF IFNKQYLWLT EKIELLICNK FTSKTCESPS LKKKKLDKCD
SSVISGYSTN NHSTFKVDKF LYENFMWILE YYSKSIFNDK SFHSTNSSIH LAFLPVSFIV
TSLPLDSKNP IYWYRVLRSI SLLLFISKGH IKKYAMRLFE FLMKATQLTN LHPTCLESWY
IYINSITDTL SLTGSTISNS SNIFLQLVPA IIDILLKMLL HFNKLEDYGK THTITYTKSC
IKDLFSYILE TINKIDKNFL LLVPIISAKE LCCIRRLIMK YLALDSTSVI NQSDEQIDIA
YEQYLIDTFA RRIDMVLLFL TPNAPHITYE KLLGSIKDLL FLHPIDSYWV LYSNIDNYVL
LRLHKLYEHS LRIISTTGSD SLSILQREGF NNGQSIKEVI NTDELNRYNI FQMSSQKENL
TFVYSTLPRK ISYSIEKSMM FLRNIAAILI GTIGAIGGEF HLQNYINNTF QDKTVSQTLL
NSATEPNEYN NIPIMLKTSI FVHDIYKLTI SLLQDYLITY AHWNVAAYAI QESLKYIGCH
YKSNKTDSPN TDTWNKFRPE IQELLEPYRT TEYRILEGTK DHILSQLRSI NSNKIENVYE
FYFWCIDKIN EATSYRNENY VINNTEVPKS IKSISILLEG CRLAALGIPS VFNYILPIVI
ECIILLCPHS DIRMLKEKLC SLVISGLQYN VKEIQTFDKK STTQKSRNIS CPLIHSVTYE
SIFIVITNLM ELYEYLLQRK VPNTLSWFSE SLLEPYLQNL YNSELDKRHH YPKKSSLSKD
NEKFRKSNET SEQMKGVSIP SNITLSLLAE ATNFKGQKHT DEITNSANKQ LPGQDISEPE
YNECEISRYI FEEVKDKENF DERITNSANS EIKNIRRRPA SNIGDRFLSS LSNRPIIPLL
ANLQFLIDVP ISILAQAALS CGAYTRALLL IERSLLMGQT ISETIYRRKS RRINNSKGSR
LMNQFGLNME YAGNAIDYLG THNFPNIQFD PLNPLGFYSA CFNYFNELDS LTPDYQNSAS
NHILYLLFRC YFGVNDIDNL LGIMKLNEDI HNGLSSSMGK DLVQALLKKD YLRGSTIYEH
ILTENRTPKF NHIFIYKSYF RCLLNAGLPQ IVLNSVLFSK KLKNIEALTT TNSIETERSL
ENQVTHISES NSEFHSSMTT VINNSSLNIY ISEALEACYM LGDWSRLEYF LSNTPESDFY
LMPFERDAQS DQLFMDLFIP TNLDRIFQKF DVYRGKLLLH LHYLQREEIT NKLIPEIKRI
KIEKSFHSNL EKARNLVYTQ LSTAWKDSYF RSFPFLEKLH ILMDIEFIFI FLTNSTNFNS
KLNSSTEKLS SKESEDEKLT NINTPKWFRI AKFFVDRVDN IQGKFETKYR LLANAKLTLE
MAGYSIEALA TLLVLTMSKM SHFNYSQTYS NFLESIDIHE PEYRYNVNSI NSNTCDTANV
SYLNSNISQL CISDNSNNFI PGAIQIHNCI TPKYIEFLLI EKQNSKKTTW TYTEMNLISL
WESSCDKCPF LVNLNRNLLI SYLNELFNRN QVNTALYVYK LCHNANLNKH ILNNSALAEL
DLIYLRWAIQ SSMISSESII SAFSNTIKFR PSCERTYFQF ATYLDHYFHL IISNRDSDCT
NSTTSLYLDT PASIYCGLGS VLQCDVIIYQ CILMYLNTLK YGNSFTYSSL SRILFLMFNH
SPLLIKRVTY SNRNSSQQPT NQSKDLLLVN NTGNIDSLTK IKNEISQVPS VLWFTVLPQL
LSRCQHSSLG TCIIQPLIAR ILRQLPHQAA WYFVAMLKSN CSDRKNTAIN ILNFSKLLSI
ELTSKEFCNN DVYISIIEDY ISIFEGLTIL AMDMSSGNSV PTQLNKRNNR SSSSRISNRS
LSLRVDFQSL YHSISNINKT NVIIPIQKQL YFFDSSLSQN TSMQFASFLP NVKQFISTQR
INSAPNDKWK IFHYSSNELI TITGMEDIIT VLSSKQKPKR IGIIGNDGHI YYYLVKSEKR
GDLRKDMRLM ELARIANQRM TRKDAELHLR TFCVIPLSEV AGIIEWVPNV VTLRSLVTNE
WKEIIGPTKF HRQLLETQDI LRQNSTNPEK LYKIFCEDIL PRYPPVLHNW FFKQFSVTSS
YNWLQAKQLY TKSTAAWSMF GYIVGLGDRH AENILIDTCN GEIIHVDFDC LFGKGFLLEI
PEIVPFRLTP NIVIAMGTCG VEGTFTGSCI SVMNVFRNPF NKTLIMTYLE AFVHDPLIEW
MRPGKTSQIL NSEGINIDPA SFAAIAKGHS HLRTIHRKLH GMVNCVAEYV KIGSTSKNSS
PEHSRRSVQE RGLGLSVESQ VIELINSAKC KHNLAVMYAG WMPQL
//
