ID B6HNC1_PENRW Unreviewed; 995 AA.
AC B6HNC1;
DT 16-DEC-2008, integrated into UniProtKB/TrEMBL.
DT 16-DEC-2008, sequence version 1.
DT 27-MAR-2024, entry version 96.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=Pc21g13710 {ECO:0000313|EMBL:CAP96268.1}, PCH_Pc21g13710
GN {ECO:0000313|EMBL:CAP96268.1};
OS Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin
OS 54-1255) (Penicillium chrysogenum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium;
OC Penicillium chrysogenum species complex.
OX NCBI_TaxID=500485 {ECO:0000313|EMBL:CAP96268.1, ECO:0000313|Proteomes:UP000000724};
RN [1] {ECO:0000313|EMBL:CAP96268.1, ECO:0000313|Proteomes:UP000000724}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255
RC {ECO:0000313|Proteomes:UP000000724};
RX PubMed=18820685; DOI=10.1038/nbt.1498;
RA van den Berg M.A., Albang R., Albermann K., Badger J.H., Daran J.-M.,
RA Driessen A.J.M., Garcia-Estrada C., Fedorova N.D., Harris D.M.,
RA Heijne W.H.M., Joardar V.S., Kiel J.A.K.W., Kovalchuk A., Martin J.F.,
RA Nierman W.C., Nijland J.G., Pronk J.T., Roubos J.A., van der Klei I.J.,
RA van Peij N.N.M.E., Veenhuis M., von Doehren H., Wagner C., Wortman J.R.,
RA Bovenberg R.A.L.;
RT "Genome sequencing and analysis of the filamentous fungus Penicillium
RT chrysogenum.";
RL Nat. Biotechnol. 26:1161-1168(2008).
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; AM920436; CAP96268.1; -; Genomic_DNA.
DR RefSeq; XP_002568388.1; XM_002568342.1.
DR AlphaFoldDB; B6HNC1; -.
DR STRING; 500485.B6HNC1; -.
DR GeneID; 8307960; -.
DR KEGG; pcs:Pc21g13710; -.
DR VEuPathDB; FungiDB:PCH_Pc21g13710; -.
DR eggNOG; KOG0433; Eukaryota.
DR HOGENOM; CLU_001493_7_2_1; -.
DR OMA; HCWRCKT; -.
DR OrthoDB; 656at2759; -.
DR BioCyc; PCHR:PC21G13710-MONOMER; -.
DR Proteomes; UP000000724; Contig Pc00c21.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000000724}.
FT DOMAIN 41..694
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 739..893
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT REGION 650..670
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 995 AA; 112352 MW; 824F788995D2EE0F CRC64;
MPELARSIPR SWTSTLTLPK SSFPPRVSPV DRTKYLKRCT DDLYAWQRRE RPADQPFVLH
DGPPYANGDL HVGHALNKIL KDIICRVQLA RGKRVRYVPG WDCHGLPIEL KALQGLRDAD
ITSETVSPAV IRTSARKLAR QTVKEQMKGF RGFAVMADWE NHWKTMDKQF EMRQLGIFRE
MVDRGLIYRK FKPVYWSPSS GTALAEAELE YKEDHTSTAA LVKFPLVDIP KYLLENPLLR
GKDLSAVIWT TTPWTLPANA AIAVSESLEY TIVLSEKHGH LLIAQSRFEY LQNMLKEDLS
VIVPSILGSE LAEKTTYRPL FKGPEAQPQP IFAADFVTAD SGSGMVHCAP GHGMDDYEAC
LARGIPVFAP VNDEGKFTDK AMPLDPTRLT GKPVLGEGNT AVLEWVESCD QLLAQHKYEH
KYPYDWRSKL PVIVRATEQW FADVADIRSS ALRALEDVRF VPETGKQRLE NFVKNRSEWC
ISRQRAWGVP IPTIYHRTTG EAVLTKDSVS HIMATIEERE IDAWWTDSAD DAAWIPPSLR
NEPGAGYRRG TDTMDVWFDS GTSWAEIDVP MEGRSHPADV YLEGTDQHRG WFQSGLLTYI
SHQLGSGQST PRAPFKNLIT HGFTLDEYGR KMSKSVGNVI HPQTIMDGTL LPPLKPRKGK
GKKQLENPEP VYDALGPDAL RMWAASSDYT RDVVIGRQVL QTVNTSLHKF RVTFKLLLGA
LADFHPENVV PYGQLQLVDR IALKHLSDMV LTSQKACDNF EFYKGVSTMN RWANLEFSAF
YMEAIKDRLY TLGEDSTSRR AAQTTLFYVY NHLQEALAPI TPVLVEETWE YTPESIRTKS
QHPLQRVISA PAPEWQDSAL DTSYQELTVV HSAIKNLQEQ ARSKKQLGSS LQSFVHIVVP
STGLFQQYLS ELPDLFVVSS ITLSSHGEAL PTEIKDAEWQ YEETFELGGQ PGKVYVYAPQ
ASKCPRCWRY AIPEPVAVEN TICDRCETVM HEANA
//
