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Database: UniProt
Entry: B6YWH0
LinkDB: B6YWH0
Original site: B6YWH0 
ID   GYAR_THEON              Reviewed;         334 AA.
AC   B6YWH0;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2009, sequence version 1.
DT   13-FEB-2019, entry version 72.
DE   RecName: Full=Glyoxylate reductase {ECO:0000255|HAMAP-Rule:MF_00776};
DE            EC=1.1.1.26 {ECO:0000255|HAMAP-Rule:MF_00776};
GN   Name=gyaR {ECO:0000255|HAMAP-Rule:MF_00776};
GN   OrderedLocusNames=TON_0945;
OS   Thermococcus onnurineus (strain NA1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus.
OX   NCBI_TaxID=523850;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NA1;
RX   PubMed=18790866; DOI=10.1128/JB.00746-08;
RA   Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H.,
RA   Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I.,
RA   Chun J., Colwell R.R., Kim S.-J., Lee J.-H.;
RT   "The complete genome sequence of Thermococcus onnurineus NA1 reveals a
RT   mixed heterotrophic and carboxydotrophic metabolism.";
RL   J. Bacteriol. 190:7491-7499(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycolate + NAD(+) = glyoxylate + H(+) + NADH;
CC         Xref=Rhea:RHEA:18229, ChEBI:CHEBI:15378, ChEBI:CHEBI:29805,
CC         ChEBI:CHEBI:36655, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.1.1.26; Evidence={ECO:0000255|HAMAP-Rule:MF_00776};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00776}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00776}.
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family. GyaR subfamily. {ECO:0000255|HAMAP-
CC       Rule:MF_00776}.
DR   EMBL; CP000855; ACJ16433.1; -; Genomic_DNA.
DR   RefSeq; WP_012571905.1; NC_011529.1.
DR   ProteinModelPortal; B6YWH0; -.
DR   SMR; B6YWH0; -.
DR   STRING; 523850.TON_0945; -.
DR   EnsemblBacteria; ACJ16433; ACJ16433; TON_0945.
DR   GeneID; 7017248; -.
DR   KEGG; ton:TON_0945; -.
DR   PATRIC; fig|523850.10.peg.953; -.
DR   eggNOG; arCOG01755; Archaea.
DR   eggNOG; COG1052; LUCA.
DR   HOGENOM; HOG000136700; -.
DR   KO; K00015; -.
DR   OMA; KWIAHNG; -.
DR   OrthoDB; 36410at2157; -.
DR   BioCyc; TONN523850:G1GAB-978-MONOMER; -.
DR   Proteomes; UP000002727; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0047964; F:glyoxylate reductase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   HAMAP; MF_00776; GyaR; 1.
DR   InterPro; IPR006139; D-isomer_2_OHA_DH_cat_dom.
DR   InterPro; IPR029753; D-isomer_DH_CS.
DR   InterPro; IPR006140; D-isomer_DH_NAD-bd.
DR   InterPro; IPR023519; Glyoxylate_reductase_GyaR.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN         1    334       Glyoxylate reductase.
FT                                /FTId=PRO_1000201582.
FT   NP_BIND     158    161       NADP. {ECO:0000255|HAMAP-Rule:MF_00776}.
FT   NP_BIND     180    182       NADP. {ECO:0000255|HAMAP-Rule:MF_00776}.
FT   NP_BIND     239    241       NADP. {ECO:0000255|HAMAP-Rule:MF_00776}.
FT   NP_BIND     288    290       NADP. {ECO:0000255|HAMAP-Rule:MF_00776}.
FT   ACT_SITE    241    241       {ECO:0000255|HAMAP-Rule:MF_00776}.
FT   ACT_SITE    270    270       {ECO:0000255|HAMAP-Rule:MF_00776}.
FT   ACT_SITE    288    288       Proton donor. {ECO:0000255|HAMAP-
FT                                Rule:MF_00776}.
SQ   SEQUENCE   334 AA;  37780 MW;  19FB2B93FD933EBF CRC64;
     MKPKVLITRK IPENGIKMLR EHFEVEVWED EHEISREVLL EKVRDVDALV TMLSERIDAE
     VFDAAPRLKI VANYAVGYDN IDIEEATKMG VYITNTPDVL TNATADMAWV LLLATARRLI
     EADKFVRSGE WKKRGVAWHP LMFLGYDVYG RTIGIVGFGR IGQAIARRAK GFGMRILYNS
     RTRKPEVEKE LGAEFMPLDE LLKESDFVVL VVPLTKETYH MINEERLKLM KPTAILVNIA
     RGKVVDTEAL VKALREGWIA GAGLDVFEEE PYYHEELFSL DNVVLAPHIG SATYGAREGM
     AELVARNLIA FKNGEVPPTL VNREVLNVRK PGFE
//
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