ID B7K553_RIPO1 Unreviewed; 676 AA.
AC B7K553;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=PCC8801_3935 {ECO:0000313|EMBL:ACK67879.1};
OS Rippkaea orientalis (strain PCC 8801 / RF-1) (Cyanothece sp. (strain PCC
OS 8801)).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Aphanothecaceae; Rippkaea; Rippkaea orientalis.
OX NCBI_TaxID=41431 {ECO:0000313|EMBL:ACK67879.1, ECO:0000313|Proteomes:UP000008204};
RN [1] {ECO:0000313|Proteomes:UP000008204}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 8801 {ECO:0000313|Proteomes:UP000008204};
RX PubMed=21972240; DOI=10.1128/mBio.00214-11;
RA Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H.,
RA Sherman L.A., Pakrasi H.B.;
RT "Novel metabolic attributes of the genus Cyanothece, comprising a group of
RT unicellular nitrogen-fixing Cyanobacteria.";
RL MBio 2:E214-E214(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP001287; ACK67879.1; -; Genomic_DNA.
DR RefSeq; WP_012597133.1; NC_011726.1.
DR AlphaFoldDB; B7K553; -.
DR STRING; 41431.PCC8801_3935; -.
DR REBASE; 19480; M.Csp8801ORF3935P.
DR KEGG; cyp:PCC8801_3935; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_012122_0_0_3; -.
DR OrthoDB; 467945at2; -.
DR Proteomes; UP000008204; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ACK67879.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000008204};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..139
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 154..452
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 676 AA; 77767 MW; 5364022DA6F0D2B5 CRC64;
MQNFGEKVSF IWSVADLIRD SFKRGKYQDV ILPFTVLRRL DCVLEPTKEQ VLEAYHKYHG
KLENLDPILC KQSGFAFYNA SNYDFGKLID DPKDLGANLK KYINSFSSNM REVLEKFDFP
NTIDKLEEAD LLFQVMEKFK TIDLHPDKVS NLEMGYIFEE LIRKFNEALD ENPGEHFTPR
EVIRLMVSLL LSQDKDSLKQ AHITRTIYDP CCGSGGMLTI AKERILELNP NATVFLFGQE
VNPETFAICK SDLYMKSEDG KDADNIKFGS TLSNDQHSDK SFDYLLANPP YGKDWKRDKD
AVETEAQKTG SRFSAGTPRI SDGQLLFLQQ MLARMKSPEN GGSRVAIVMN GSPLFTGDAG
SGESEIRRWI LENDWLEAII ALPEQLFYNT GISTYIWILS NKKLLQKKEK VQLINGSDFW
VAMRKSLGDK RREISTEHIE KITAIFQDFE VSEVSKTFNS TDFGYRKITI ERPLRLNFQV
IPERIERVKE QTAFINLAVS KKKNPEMRKI EEDAGREQQK LILGVLNGLS DELYKDRKPL
ELLLKKAFKV ENVAVKGALF KAILTGLSEK DETAEICRDK DGNPEPDTEL RDTENVPLDE
DIYDYFEREV KPHVSDAWIN ETVRDSKDSG VGKVGYEINF NRYFYQYQPP RELSEIEKDI
QQVEGEILAM LKEMRE
//
