ID NANT_ECO8A Reviewed; 496 AA.
AC B7M0T6;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 78.
DE RecName: Full=Sialic acid transporter NanT {ECO:0000255|HAMAP-Rule:MF_01238};
DE AltName: Full=Sialic acid permease {ECO:0000255|HAMAP-Rule:MF_01238};
DE AltName: Full=Sialic acid/H(+) symporter {ECO:0000255|HAMAP-Rule:MF_01238};
GN Name=nanT {ECO:0000255|HAMAP-Rule:MF_01238}; OrderedLocusNames=ECIAI1_3366;
OS Escherichia coli O8 (strain IAI1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=585034;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IAI1;
RX PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H.,
RA Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E.,
RA Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C.,
RA Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S.,
RA Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J.,
RA Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT "Organised genome dynamics in the Escherichia coli species results in
RT highly diverse adaptive paths.";
RL PLoS Genet. 5:E1000344-E1000344(2009).
CC -!- FUNCTION: Catalyzes the proton-dependent transport of sialic acid.
CC {ECO:0000255|HAMAP-Rule:MF_01238}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + N-acetylneuraminate(in) = H(+)(out) + N-
CC acetylneuraminate(out); Xref=Rhea:RHEA:28987, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:35418; Evidence={ECO:0000255|HAMAP-Rule:MF_01238};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01238}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01238}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sialate:H(+)
CC symporter (SHS) (TC 2.A.1.12) family. {ECO:0000255|HAMAP-
CC Rule:MF_01238}.
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DR EMBL; CU928160; CAR00180.1; -; Genomic_DNA.
DR RefSeq; WP_000108459.1; NC_011741.1.
DR AlphaFoldDB; B7M0T6; -.
DR SMR; B7M0T6; -.
DR GeneID; 75206074; -.
DR KEGG; ecr:ECIAI1_3366; -.
DR HOGENOM; CLU_001265_46_8_6; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015538; F:sialic acid:proton symporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR CDD; cd17316; MFS_SV2_like; 1.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 2.
DR HAMAP; MF_01238; MFS_NanT; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004742; SA_transporter.
DR NCBIfam; TIGR00891; 2A0112; 1.
DR PANTHER; PTHR23508; CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG; 1.
DR PANTHER; PTHR23508:SF3; SIALIC ACID TRANSPORTER NANT; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..496
FT /note="Sialic acid transporter NanT"
FT /id="PRO_1000214045"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 353..375
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
SQ SEQUENCE 496 AA; 53581 MW; 92B71FE40ACB0300 CRC64;
MSTTTQNIPW YRHLNRAQWR AFSAAWLGYL LDGFDFVLIA LVLTEVQGEF GLTTVQAASL
ISAAFISRWF GGLMLGAMGD RYGRRLAMVT SIVLFSAGTL ACGFAPGYIT MFIARLVIGM
GMAGEYGSSA TYVIESWPKH LRNKASGFLI SGFSVGAVVA AQVYSLVVPV WGWRALFFIG
ILPIIFALWL RKNIPEAEDW KEKHAGKAPV RTMVDILYRG EHRIANIVMT LAAATALWFC
FAGNLQNAAI VAVLGLLCAA IFISFMVQST GKRWPTGVML MVVVLFAFLY SWPIQALLPT
YLKTDLAYNP HTVANVLFFS GFGAAVGCCV GGFLGDWLGT RKAYVCSLLA SQLLIIPVFA
IGGANVWVLG LLLFFQQMLG QGIAGILPKL IGGYFDTDQR AAGLGFTYNV GALGGALAPI
IGALIAQRLD LGTALASLSF SLTFVVILLI GLDMPSRVQR WLRPEALRTH DAIDGKPFSG
AVPFGSAKND LVKTKS
//
