ID B7P7Z6_IXOSC Unreviewed; 546 AA.
AC B7P7Z6;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 24-JAN-2024, entry version 82.
DE RecName: Full=CTP synthase {ECO:0000256|RuleBase:RU810713};
DE EC=6.3.4.2 {ECO:0000256|RuleBase:RU810713};
DE AltName: Full=UTP--ammonia ligase {ECO:0000256|RuleBase:RU810713};
GN ORFNames=IscW_ISCW017186 {ECO:0000313|EMBL:EEC02718.1};
OS Ixodes scapularis (Black-legged tick) (Deer tick).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX NCBI_TaxID=6945;
RN [1] {ECO:0000313|EMBL:EEC02718.1, ECO:0000313|Proteomes:UP000001555}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Wikel {ECO:0000313|Proteomes:UP000001555}, and Wikel colony
RC {ECO:0000313|EMBL:EEC02718.1};
RG Ixodes scapularis Genome Project Consortium;
RA Caler E., Hannick L.I., Bidwell S., Joardar V., Thiagarajan M., Amedeo P.,
RA Galinsky K.J., Schobel S., Inman J., Hostetler J., Miller J., Hammond M.,
RA Megy K., Lawson D., Kodira C., Sutton G., Meyer J., Hill C.A., Birren B.,
RA Nene V., Collins F., Alarcon-Chaidez F., Wikel S., Strausberg R.;
RT "Annotation of Ixodes scapularis.";
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:ISCW017186-PA}
RP IDENTIFICATION.
RC STRAIN=wikel {ECO:0000313|EnsemblMetazoa:ISCW017186-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- FUNCTION: Catalyzes the ATP-dependent amination of UTP to CTP with
CC either L-glutamine or ammonia as the source of nitrogen.
CC {ECO:0000256|RuleBase:RU810713}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-
CC glutamate + phosphate; Xref=Rhea:RHEA:26426, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37563, ChEBI:CHEBI:43474, ChEBI:CHEBI:46398,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.4.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000314,
CC ECO:0000256|RuleBase:RU810713};
CC -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway;
CC CTP from UDP: step 2/2. {ECO:0000256|ARBA:ARBA00005171,
CC ECO:0000256|RuleBase:RU810713}.
CC -!- SIMILARITY: Belongs to the CTP synthase family.
CC {ECO:0000256|ARBA:ARBA00007533, ECO:0000256|RuleBase:RU810713}.
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DR EMBL; ABJB010033127; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010397788; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010446140; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010514985; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010684286; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010860393; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DS654628; EEC02718.1; -; Genomic_DNA.
DR RefSeq; XP_002400452.1; XM_002400408.1.
DR AlphaFoldDB; B7P7Z6; -.
DR STRING; 6945.B7P7Z6; -.
DR PaxDb; 6945-B7P7Z6; -.
DR EnsemblMetazoa; ISCW017186-RA; ISCW017186-PA; ISCW017186.
DR KEGG; isc:IscW_ISCW017186; -.
DR VEuPathDB; VectorBase:ISCP_013303; -.
DR VEuPathDB; VectorBase:ISCW017186; -.
DR HOGENOM; CLU_011675_5_0_1; -.
DR InParanoid; B7P7Z6; -.
DR OMA; EFNNAYR; -.
DR UniPathway; UPA00159; UER00277.
DR Proteomes; UP000001555; Unassembled WGS sequence.
DR GO; GO:0097268; C:cytoophidium; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003883; F:CTP synthase activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IBA:GO_Central.
DR GO; GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006241; P:CTP biosynthetic process; IBA:GO_Central.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0019856; P:pyrimidine nucleobase biosynthetic process; IBA:GO_Central.
DR CDD; cd03113; CTPS_N; 1.
DR CDD; cd01746; GATase1_CTP_Synthase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR004468; CTP_synthase.
DR InterPro; IPR017456; CTP_synthase_N.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR033828; GATase1_CTP_Synthase.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11550; CTP SYNTHASE; 1.
DR PANTHER; PTHR11550:SF0; CTP SYNTHASE-RELATED; 1.
DR Pfam; PF06418; CTP_synth_N; 1.
DR Pfam; PF00117; GATase; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 1: Evidence at protein level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU810713};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962,
KW ECO:0000256|PROSITE-ProRule:PRU00605};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU810713};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU810713};
KW Proteomics identification {ECO:0007829|PeptideAtlas:B7P7Z6};
KW Pyrimidine biosynthesis {ECO:0000256|ARBA:ARBA00022975,
KW ECO:0000256|RuleBase:RU810713};
KW Reference proteome {ECO:0000313|Proteomes:UP000001555}.
FT DOMAIN 2..186
FT /note="CTP synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF06418"
FT DOMAIN 285..513
FT /note="Glutamine amidotransferase"
FT /evidence="ECO:0000259|Pfam:PF00117"
FT ACT_SITE 373
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 500
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 502
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ SEQUENCE 546 AA; 61708 MW; 61F05C22B135B06B CRC64;
MKYILVTGGV ISGIGKGVIA SSTGTILKAC GLQVTSIKID PSINIDAGTF SPYEHGEVYV
LDDGGEVDLD LGNYERFLDI TLHRDNNITT GKIYQHVINK ERRGDYLGKT VQVVPHITDA
IQEWVERVAR IPVGESAQTP EVCIIELGGT IGDIEGMPFV EAFRQFQFRV KRENFCCVHV
SLVPQIVCRS ENEITKAVKE KISNFCHVEP RQVLCMHDVP SIYHVPIFME EQGIVELFTE
RLQLQVPTVP RTRRLMYKWR DLALKAERLM REVTIALVGK YTQLEDAYSS VIKALQHSAL
ACNHKLKIKY IDAEELEPEM KRDDPVKFHA AWQNLCRSDG ILVPGGFGSR GVEGKIAAIE
WARKTKKPFL GVCLGLQCAV IEFCRNVLGW ADANSTEINP ESKYPVIIEM PEHHVGQMGG
TMRLGKRKTI FNTHDSILRK LYMNADVIEE RHRHRYEVNP AFASDLEKAG LHFVGKDDTG
KRMEIIEFPD HPYFVGVQFH PEYISRPLKP SPPYLGLVLA SCGRLSNFLV RGCRMSPRHP
KQSEFL
//
