ID B7PT92_IXOSC Unreviewed; 468 AA.
AC B7PT92;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 27-MAR-2024, entry version 84.
DE SubName: Full=6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, putative {ECO:0000313|EMBL:EEC09814.1, ECO:0000313|EnsemblMetazoa:ISCW008488-PA};
DE EC=3.1.3.46 {ECO:0000313|EMBL:EEC09814.1};
DE Flags: Fragment;
GN Name=8031022 {ECO:0000313|EnsemblMetazoa:ISCW008488-PA};
GN ORFNames=IscW_ISCW008488 {ECO:0000313|EMBL:EEC09814.1};
OS Ixodes scapularis (Black-legged tick) (Deer tick).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX NCBI_TaxID=6945;
RN [1] {ECO:0000313|EMBL:EEC09814.1, ECO:0000313|Proteomes:UP000001555}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Wikel {ECO:0000313|Proteomes:UP000001555}, and Wikel colony
RC {ECO:0000313|EMBL:EEC09814.1};
RG Ixodes scapularis Genome Project Consortium;
RA Caler E., Hannick L.I., Bidwell S., Joardar V., Thiagarajan M., Amedeo P.,
RA Galinsky K.J., Schobel S., Inman J., Hostetler J., Miller J., Hammond M.,
RA Megy K., Lawson D., Kodira C., Sutton G., Meyer J., Hill C.A., Birren B.,
RA Nene V., Collins F., Alarcon-Chaidez F., Wikel S., Strausberg R.;
RT "Annotation of Ixodes scapularis.";
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:ISCW008488-PA}
RP IDENTIFICATION.
RC STRAIN=wikel {ECO:0000313|EnsemblMetazoa:ISCW008488-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- SIMILARITY: In the C-terminal section; belongs to the phosphoglycerate
CC mutase family. {ECO:0000256|ARBA:ARBA00008408}.
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DR EMBL; ABJB010090174; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010448118; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; ABJB010800621; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DS784338; EEC09814.1; -; Genomic_DNA.
DR RefSeq; XP_002404032.1; XM_002403988.1.
DR AlphaFoldDB; B7PT92; -.
DR STRING; 6945.B7PT92; -.
DR PaxDb; 6945-B7PT92; -.
DR EnsemblMetazoa; ISCW008488-RA; ISCW008488-PA; ISCW008488.
DR KEGG; isc:IscW_ISCW008488; -.
DR VEuPathDB; VectorBase:ISCI008488; -.
DR VEuPathDB; VectorBase:ISCP_002819; -.
DR VEuPathDB; VectorBase:ISCW008488; -.
DR HOGENOM; CLU_006383_1_1_1; -.
DR InParanoid; B7PT92; -.
DR OMA; RWIQERC; -.
DR OrthoDB; 2013830at2759; -.
DR Proteomes; UP000001555; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003873; F:6-phosphofructo-2-kinase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; IBA:GO_Central.
DR GO; GO:0006003; P:fructose 2,6-bisphosphate metabolic process; IBA:GO_Central.
DR GO; GO:0006000; P:fructose metabolic process; IEA:InterPro.
DR CDD; cd07067; HP_PGM_like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 3.40.50.1240; Phosphoglycerate mutase-like; 1.
DR InterPro; IPR003094; 6Pfruct_kin.
DR InterPro; IPR013079; 6Phosfructo_kin.
DR InterPro; IPR013078; His_Pase_superF_clade-1.
DR InterPro; IPR029033; His_PPase_superfam.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001345; PG/BPGM_mutase_AS.
DR PANTHER; PTHR10606:SF44; 6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM; 1.
DR PANTHER; PTHR10606; 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE; 1.
DR Pfam; PF01591; 6PF2K; 1.
DR Pfam; PF00300; His_Phos_1; 1.
DR PIRSF; PIRSF000709; 6PFK_2-Ptase; 1.
DR PRINTS; PR00991; 6PFRUCTKNASE.
DR SMART; SM00855; PGAM; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF53254; Phosphoglycerate mutase-like; 1.
DR PROSITE; PS00175; PG_MUTASE; 1.
PE 1: Evidence at protein level;
KW Hydrolase {ECO:0000313|EMBL:EEC09814.1};
KW Kinase {ECO:0000313|EMBL:EEC09814.1};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Proteomics identification {ECO:0007829|PeptideAtlas:B7PT92};
KW Reference proteome {ECO:0000313|Proteomes:UP000001555};
KW Transferase {ECO:0000313|EMBL:EEC09814.1}.
FT DOMAIN 33..248
FT /note="6-phosphofructo-2-kinase"
FT /evidence="ECO:0000259|Pfam:PF01591"
FT REGION 445..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 447..468
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 256
FT /note="Tele-phosphohistidine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-1"
FT ACT_SITE 325
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-1"
FT BINDING 255..262
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-2"
FT BINDING 305
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-2"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:EEC09814.1"
SQ SEQUENCE 468 AA; 54323 MW; C8DC1496E68A1BEF CRC64;
LFVYLFSSVY VRASVRVYSE VCTHLPAAGD RTNYVNVPHI IAMVGLPARG KTYIAKKLTR
YLNWIGIQTR VFNVGEYRRQ ATEAYRSHDF FRADNAEAMA IRSKCALDAL EDMCKWLETE
GEVAVYDATN TTFERRKLIY DVVCERFGFK LFFVESYCDD PKIIEANIRE VKINSPDYRD
MNKDDALLDF VHRIEHYKAC YQTLDEVQEK QYSFMKIFNT GQKVVVHRHE GHIQSRVVYY
LMNIHIMPRS IYLTRHGEST LNLQGRIGGD ADLSERGSEY AVALAKFIKR QAIPRLRVWT
SQLKRTIQTA AGIDAPQERW KALNEIDAGI CEEMTYEEIQ EKFPEEFAAR DQDKFHYRYP
RGESYEDLVA RLEPVIMELE RQENVLVVAH QAVLRCLLAY FLDKNSEELP YLRVPLHGII
KLTPMAYGCE VKKFSVPIAA VDTHREKPSN CSPKRSREDA LKTVPPHM
//
