ID B7RVB5_9GAMM Unreviewed; 1115 AA.
AC B7RVB5;
DT 10-FEB-2009, integrated into UniProtKB/TrEMBL.
DT 10-FEB-2009, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=GPB2148_3816 {ECO:0000313|EMBL:EEB79967.1};
OS marine gamma proteobacterium HTCC2148.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales; Halieaceae.
OX NCBI_TaxID=247634 {ECO:0000313|EMBL:EEB79967.1, ECO:0000313|Proteomes:UP000002793};
RN [1] {ECO:0000313|Proteomes:UP000002793}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTCC2148 {ECO:0000313|Proteomes:UP000002793};
RX PubMed=20472793; DOI=10.1128/jb.00511-10;
RA Thrash J.C., Cho J.C., Ferriera S., Johnson J., Vergin K.L.,
RA Giovannoni S.J.;
RT "Genome sequences of strains HTCC2148 and HTCC2080, belonging to the
RT OM60/NOR5 clade of the Gammaproteobacteria.";
RL J. Bacteriol. 192:3842-3843(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR EMBL; DS999223; EEB79967.1; -; Genomic_DNA.
DR AlphaFoldDB; B7RVB5; -.
DR STRING; 247634.GPB2148_3816; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_009270_0_0_6; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000002793; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 2.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000002793}.
FT DOMAIN 2..485
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 486..766
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 23..30
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1115 AA; 123573 MW; 7FC698807F4CEB16 CRC64;
MTIADANDRA EALDVVRSFC VTAPAGSGKT ELLIQRFLAL LARVKRPEQV LAITFTRKAA
AEMRERVLQA LQAARDKVAV EGEHQRVTRD LALAALESSD REQWHLARDI SRLNIKTIDS
FCAGLTRQMP ILSRFGGQAQ AVDDATELYK EAVSELFSIA GTSRSEATDL DILLLHFDNN
WDRLRELLVT MLGKRDQWHL YMGAKRSPEQ AEVKLRATVE SVVTEGLQDL STVLDPWQDE
IFDLLCYARE NLGEGIPDEF PAASADNMEL WRFMGQLFLT QGGSFRKSVN VRNGFPTGDD
DAKARKAHFV DLVARMGQVK GLAGELGGLA WLPRMDNHND AWQMVLHLSH VLPLLAACLL
LVFERRSLVD HSQVALSALD ALGEDDEPTE LALRLDYSIE HILIDEFQDT AINQYNLVSK
LTRGWGQHNA LNPAAPRTLF IVGDGMQSIY GFRNANVGLF LKARQEGFNG VTLQALSLRC
NFRSEQGIVD WVNDSFRDAF PAEDNVYRGR VSFTDAIAVK PAGAELAVSL NGFWGEQAKE
QEAQWLVEQV ALGLADPNLG SIAILGRSRP QLAPILELLR QRDIPFASQE MDALAGSPAI
VDLMTLCRAL VNPGDRVAWY AVLRAPWCAL NLADMKCLAD SAQDPRWQNV YGALCNRELP
AGLSAEGKQR LLHVGQCLLW AQQRRDRLGL RAWIEQLWLH LGGPACFTQQ RFLADAERFL
SLLQLADAEG KGLNIDWLEQ RISRLYASGD APDARLQVMT LHKSKGLEFD WVLIPALARA
TRGDSRDILL WDEHNSKTGE LGFMLAADDH SDDKTPGLYN FLKKQRKEKS RLETTRLLYV
GATRAVKKLT LSACLGGGED AGPQDEPEFK PPGDGALLAP IWATFERQAV VHVPMTSVQP
QSKISRGLLR LSQSPATLPL MPKDSPQGAN IPDPALNWLD RYVGTVIHEV LEQLSLLASL
PDELPVRLAE LARHSLSSMG LAEEQLEAAF GRVSHAVAST LADESGGRWV LSAQHAQAHS
ELTLTIQEAG QPRDIVIDRT FVDRDTGIRW IIDYKSSTPE AGVSAASFLQ VEAGRYKNQL
SCYRDAMSEI GPEPVRCALY FTGMACLHHL SELDR
//
