ID B8C4Z8_THAPS Unreviewed; 1671 AA.
AC B8C4Z8;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 24-JAN-2024, entry version 75.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN Name=RPA1 {ECO:0000313|EMBL:EED91031.1};
GN ORFNames=THAPSDRAFT_40884 {ECO:0000313|EMBL:EED91031.1};
OS Thalassiosira pseudonana (Marine diatom) (Cyclotella nana).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC Coscinodiscophyceae; Thalassiosirophycidae; Thalassiosirales;
OC Thalassiosiraceae; Thalassiosira.
OX NCBI_TaxID=35128 {ECO:0000313|EMBL:EED91031.1, ECO:0000313|Proteomes:UP000001449};
RN [1] {ECO:0000313|EMBL:EED91031.1, ECO:0000313|Proteomes:UP000001449}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1335 {ECO:0000313|EMBL:EED91031.1};
RX PubMed=15459382; DOI=10.1126/science.1101156;
RA Armbrust E.V., Berges J.A., Bowler C., Green B.R., Martinez D.,
RA Putnam N.H., Zhou S., Allen A.E., Apt K.E., Bechner M., Brzezinski M.A.,
RA Chaal B.K., Chiovitti A., Davis A.K., Demarest M.S., Detter J.C.,
RA Glavina T., Goodstein D., Hadi M.Z., Hellsten U., Hildebrand M.,
RA Jenkins B.D., Jurka J., Kapitonov V.V., Kroger N., Lau W.W., Lane T.W.,
RA Larimer F.W., Lippmeier J.C., Lucas S., Medina M., Montsant A., Obornik M.,
RA Parker M.S., Palenik B., Pazour G.J., Richardson P.M., Rynearson T.A.,
RA Saito M.A., Schwartz D.C., Thamatrakoln K., Valentin K., Vardi A.,
RA Wilkerson F.P., Rokhsar D.S.;
RT "The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and
RT metabolism.";
RL Science 306:79-86(2004).
RN [2] {ECO:0000313|EMBL:EED91031.1, ECO:0000313|Proteomes:UP000001449}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1335 {ECO:0000313|EMBL:EED91031.1};
RX PubMed=18923393; DOI=10.1038/nature07410;
RA Bowler C., Allen A.E., Badger J.H., Grimwood J., Jabbari K., Kuo A.,
RA Maheswari U., Martens C., Maumus F., Otillar R.P., Rayko E., Salamov A.,
RA Vandepoele K., Beszteri B., Gruber A., Heijde M., Katinka M., Mock T.,
RA Valentin K., Verret F., Berges J.A., Brownlee C., Cadoret J.P.,
RA Chiovitti A., Choi C.J., Coesel S., De Martino A., Detter J.C., Durkin C.,
RA Falciatore A., Fournet J., Haruta M., Huysman M.J., Jenkins B.D.,
RA Jiroutova K., Jorgensen R.E., Joubert Y., Kaplan A., Kroger N., Kroth P.G.,
RA La Roche J., Lindquist E., Lommer M., Martin-Jezequel V., Lopez P.J.,
RA Lucas S., Mangogna M., McGinnis K., Medlin L.K., Montsant A.,
RA Oudot-Le Secq M.P., Napoli C., Obornik M., Parker M.S., Petit J.L.,
RA Porcel B.M., Poulsen N., Robison M., Rychlewski L., Rynearson T.A.,
RA Schmutz J., Shapiro H., Siaut M., Stanley M., Sussman M.R., Taylor A.R.,
RA Vardi A., von Dassow P., Vyverman W., Willis A., Wyrwicz L.S.,
RA Rokhsar D.S., Weissenbach J., Armbrust E.V., Green B.R., Van de Peer Y.,
RA Grigoriev I.V.;
RT "The Phaeodactylum genome reveals the evolutionary history of diatom
RT genomes.";
RL Nature 456:239-244(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
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DR EMBL; CM000643; EED91031.1; -; Genomic_DNA.
DR RefSeq; XP_002290924.1; XM_002290888.1.
DR STRING; 35128.B8C4Z8; -.
DR PaxDb; 35128-Thaps40884; -.
DR EnsemblProtists; EED91031; EED91031; THAPSDRAFT_40884.
DR GeneID; 7451111; -.
DR KEGG; tps:THAPSDRAFT_40884; -.
DR eggNOG; KOG0262; Eukaryota.
DR HOGENOM; CLU_000487_2_2_1; -.
DR InParanoid; B8C4Z8; -.
DR OMA; NREDYQQ; -.
DR Proteomes; UP000001449; Chromosome 6.
DR GO; GO:0005736; C:RNA polymerase I complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006360; P:transcription by RNA polymerase I; IEA:GOC.
DR CDD; cd02735; RNAP_I_Rpa1_C; 1.
DR CDD; cd01435; RNAP_I_RPA1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.70.2850; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR047107; DNA-dir_RNA_pol1_lsu_C.
DR InterPro; IPR015699; DNA-dir_RNA_pol1_lsu_N.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF11; DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000001449};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 347..669
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 260..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 855..875
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1372..1470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..283
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1404..1454
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1671 AA; 183826 MW; 411E97A4D34A2B93 CRC64;
MANLNQAIIR HQPTSVQFGV YTDDEVRQRS VCEISSSVAY DALNTPLPRG LYDPLLGPTA
GGGGGASAYC ITCGNVQNLC PGHFGHIELC VPVYHPLFFP KLLLFMKMKC LACHHFRLSK
RACRVFCTKL HLVDVGRMGE AMGLDEEMAA LASKASVGGG DGKMSKQVKQ EMMSSAAVLI
DELLDQKMAL RPPDGQSAEE VTHTMHERAV RRKILKEFQQ ACTKCLKCAN CNAFSNKIRH
DQFNKMFQVA LPARNAKVSD VSREDFPANT KGKKKKKSTS SSTVDEEIDP TGGPISRKAT
VSTTKNIEAS KQDNFMHALE VEAQCRLTWN NEPFLCSKFF GWGKGYTLFF LRAVPVPPSR
FRPPVIMGNM TVEHSQNFYL SKVLELNARI RSAFATIHEL TQEEADLKNT DKGQANMLEI
WVDLQTTVNC FMDSTRDPKG TANNAPNGIR QLLEKKEGIF RKHMMGKRVN FACRSVISPD
PYIGTNEIGL PLYFAKTLTF PTPVTALNIA EMRKLVQRGP LQYPGAVWVE FPNGQRIDLS
KMKEGNRHAI AARLLADNGV VKVGRQLRDG DMVLMNRQPT LHKPGIMAHR VRVLFSPTQN
TLRMHYANCN TYNADYDGDE MNCHFPQSYL AAAESQFIAG TDLQFIVPTD GSPLRGLIQD
HVDAGVKLTC MNTFIEREVY QQLLFAALGS LPGLELIRSD ANIELLPPAI RKPKELWTGK
QVISTLLNHL RKGNDRDEDP SFNFPGLSME RKAKTPATAF GASWNEHMVI VRDGDLVQGI
LDKAAFGASE FSLVHAVYEA YGPSRAGLLL NALGRLFTAY IQYYTGHSCR MEDLILTPDA
DEKRRQLVKV ESDGGKVEIP PVAQNPKSKQ PLKPHEKATV ASKIGELLSG GDGKENAAAL
DGFMQSQVNP LASDIIKICL PDGLAVPFPE NTFGLMTTTG AKGSMVNQSQ VSCSLGQQAL
EGRRVPRMSS GRTLPSFAPY DPNPRADGFI TDRFLTGVRP QEYYFHCMAG REGLVDTAVK
TSRSGYLQRC LIKHLEELKV SYDHTVRDGE GGVVQFVYGE DGIDPTKAAH LDCESRTFQF
LARNHESLKK RYPALPGSTL DIAVKDSRRA QETTPAESFH CEIIKPGVPD PIVSDTAREN
GAHRLGSSGA CVSEHVANLA SNAMKDTGVK KALENSGLSK EGFSALVAAK YSSALAHPGE
AVGSIAAQSI GEPSTQMTLN TFHLAGAGAN VTLGIPRLRE IIMTASKELK TPTMSIPLSE
SVSDREALRL TRYFSKVSLM DLLASHGGIT VREVLEKGAG SNWERCYYVT LKLHPAERIN
EAFGLRLEDI AAVVTKTFIP KLARVMKAEM KRNETNEGYA SIEVIGGAST DFMESDVSGG
ESSKSKASKR PKDDEYDDEV ANDEDGVTGT RFGHRKEMTS YGDMDDDDKY IAKRSAKDDS
DEDEDNRLPA VITEGEESDD ELESKSSNTA RISRSKNSII LDPLRVDPST CPLLMVGLVE
RAAETTIVRA RPKINEGFVN NEEGRGRCLQ TAGCNFEEMW SLDEGVVDHN KLVSNDIWGI
RCAYGVEAAR MSIADQIRGV FAVYGISVDP RHLSLIADFM TYDGEYKPMN RIGMADISST
FLQMSFESTS VFMVDAALHK RNDPMMSPSA NIVMGHPIRH GTGAFECIAK A
//
