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Database: UniProt
Entry: B8C5U2_THAPS
LinkDB: B8C5U2_THAPS
Original site: B8C5U2_THAPS 
ID   B8C5U2_THAPS            Unreviewed;       999 AA.
AC   B8C5U2;
DT   03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT   03-MAR-2009, sequence version 1.
DT   08-MAY-2019, entry version 68.
DE   RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE            EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
DE   Flags: Fragment;
GN   Name=DPO1 {ECO:0000313|EMBL:EED91181.1};
GN   ORFNames=THAPSDRAFT_35189 {ECO:0000313|EMBL:EED91181.1};
OS   Thalassiosira pseudonana (Marine diatom) (Cyclotella nana).
OC   Eukaryota; Stramenopiles; Bacillariophyta; Coscinodiscophyceae;
OC   Thalassiosirophycidae; Thalassiosirales; Thalassiosiraceae;
OC   Thalassiosira.
OX   NCBI_TaxID=35128 {ECO:0000313|Proteomes:UP000001449};
RN   [1] {ECO:0000313|EMBL:EED91181.1, ECO:0000313|Proteomes:UP000001449}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCMP1335 {ECO:0000313|EMBL:EED91181.1,
RC   ECO:0000313|Proteomes:UP000001449};
RX   PubMed=15459382; DOI=10.1126/science.1101156;
RA   Armbrust E.V., Berges J.A., Bowler C., Green B.R., Martinez D.,
RA   Putnam N.H., Zhou S., Allen A.E., Apt K.E., Bechner M.,
RA   Brzezinski M.A., Chaal B.K., Chiovitti A., Davis A.K., Demarest M.S.,
RA   Detter J.C., Glavina T., Goodstein D., Hadi M.Z., Hellsten U.,
RA   Hildebrand M., Jenkins B.D., Jurka J., Kapitonov V.V., Kroger N.,
RA   Lau W.W., Lane T.W., Larimer F.W., Lippmeier J.C., Lucas S.,
RA   Medina M., Montsant A., Obornik M., Parker M.S., Palenik B.,
RA   Pazour G.J., Richardson P.M., Rynearson T.A., Saito M.A.,
RA   Schwartz D.C., Thamatrakoln K., Valentin K., Vardi A., Wilkerson F.P.,
RA   Rokhsar D.S.;
RT   "The genome of the diatom Thalassiosira pseudonana: ecology,
RT   evolution, and metabolism.";
RL   Science 306:79-86(2004).
RN   [2] {ECO:0000313|EMBL:EED91181.1, ECO:0000313|Proteomes:UP000001449}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCMP1335 {ECO:0000313|EMBL:EED91181.1,
RC   ECO:0000313|Proteomes:UP000001449};
RX   PubMed=18923393; DOI=10.1038/nature07410;
RA   Bowler C., Allen A.E., Badger J.H., Grimwood J., Jabbari K., Kuo A.,
RA   Maheswari U., Martens C., Maumus F., Otillar R.P., Rayko E.,
RA   Salamov A., Vandepoele K., Beszteri B., Gruber A., Heijde M.,
RA   Katinka M., Mock T., Valentin K., Verret F., Berges J.A., Brownlee C.,
RA   Cadoret J.P., Chiovitti A., Choi C.J., Coesel S., De Martino A.,
RA   Detter J.C., Durkin C., Falciatore A., Fournet J., Haruta M.,
RA   Huysman M.J., Jenkins B.D., Jiroutova K., Jorgensen R.E., Joubert Y.,
RA   Kaplan A., Kroger N., Kroth P.G., La Roche J., Lindquist E.,
RA   Lommer M., Martin-Jezequel V., Lopez P.J., Lucas S., Mangogna M.,
RA   McGinnis K., Medlin L.K., Montsant A., Oudot-Le Secq M.P., Napoli C.,
RA   Obornik M., Parker M.S., Petit J.L., Porcel B.M., Poulsen N.,
RA   Robison M., Rychlewski L., Rynearson T.A., Schmutz J., Shapiro H.,
RA   Siaut M., Stanley M., Sussman M.R., Taylor A.R., Vardi A.,
RA   von Dassow P., Vyverman W., Willis A., Wyrwicz L.S., Rokhsar D.S.,
RA   Weissenbach J., Armbrust E.V., Green B.R., Van de Peer Y.,
RA   Grigoriev I.V.;
RT   "The Phaeodactylum genome reveals the evolutionary history of diatom
RT   genomes.";
RL   Nature 456:239-244(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-
CC         COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7;
CC         Evidence={ECO:0000256|RuleBase:RU000442};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|RuleBase:RU000442};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU000442}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC       {ECO:0000256|RuleBase:RU000442}.
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DR   EMBL; CM000643; EED91181.1; -; Genomic_DNA.
DR   RefSeq; XP_002291074.1; XM_002291038.1.
DR   STRING; 35128.Thaps35189; -.
DR   EnsemblProtists; EED91181; EED91181; THAPSDRAFT_35189.
DR   GeneID; 7442345; -.
DR   KEGG; tps:THAPSDRAFT_35189; -.
DR   eggNOG; KOG0969; Eukaryota.
DR   eggNOG; COG0417; LUCA.
DR   HOGENOM; HOG000036616; -.
DR   InParanoid; B8C5U2; -.
DR   KO; K02327; -.
DR   Proteomes; UP000001449; Chromosome 6.
DR   GO; GO:0043625; C:delta DNA polymerase complex; IBA:GO_Central.
DR   GO; GO:0008296; F:3'-5'-exodeoxyribonuclease activity; IBA:GO_Central.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006287; P:base-excision repair, gap-filling; IBA:GO_Central.
DR   GO; GO:0045004; P:DNA replication proofreading; IBA:GO_Central.
DR   GO; GO:0006261; P:DNA-dependent DNA replication; IBA:GO_Central.
DR   GO; GO:0006297; P:nucleotide-excision repair, DNA gap filling; IBA:GO_Central.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR042087; DNA_pol_B_C.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR025687; Znf-C4pol.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   Pfam; PF14260; zf-C4pol; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|RuleBase:RU000442};
KW   Complete proteome {ECO:0000313|Proteomes:UP000001449};
KW   DNA replication {ECO:0000256|RuleBase:RU000442};
KW   DNA-binding {ECO:0000256|RuleBase:RU000442};
KW   DNA-directed DNA polymerase {ECO:0000256|RuleBase:RU000442};
KW   Iron {ECO:0000256|RuleBase:RU000442};
KW   Iron-sulfur {ECO:0000256|RuleBase:RU000442};
KW   Metal-binding {ECO:0000256|RuleBase:RU000442};
KW   Nucleotidyltransferase {ECO:0000256|RuleBase:RU000442,
KW   ECO:0000313|EMBL:EED91181.1};
KW   Nucleus {ECO:0000256|RuleBase:RU000442};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001449};
KW   Transferase {ECO:0000256|RuleBase:RU000442,
KW   ECO:0000313|EMBL:EED91181.1}; Zinc {ECO:0000256|RuleBase:RU000442};
KW   Zinc-finger {ECO:0000256|RuleBase:RU000442}.
FT   DOMAIN       12    373       DNA_pol_B_exo1. {ECO:0000259|Pfam:
FT                                PF03104}.
FT   DOMAIN      437    869       DNA_pol_B. {ECO:0000259|Pfam:PF00136}.
FT   DOMAIN      908    980       zf-C4pol. {ECO:0000259|Pfam:PF14260}.
FT   NON_TER       1      1       {ECO:0000313|EMBL:EED91181.1}.
SQ   SEQUENCE   999 AA;  112481 MW;  C9EF696EF2AD7498 CRC64;
     GAVPVIRLYG VNETGNSVAV FIHGFTPYGY FALPRGLRAK LGSQFGKGLQ VNGGNGQQPQ
     ACLGVQFLKD RQSIMGYDPS HTKFLKVYLG MPGMIPKLKS IMEEGLVLPG IVRVDANGNS
     VGVNESMIFQ PYECNVPFVL RYMIDRDITG ASWLSLPKGT YRLRQTENEK GTHCQIEADI
     CFNEIIPRKP EGEWSKVAPL RILSFDIECQ GRKGYFPEAE HDPVIQIANC LSVYGDKAPI
     VQNIFTLKGC LPIVGAQVIS SETEQEMLLK WRTFLHASDA DIFTGYNVQN FDIPYLLDRA
     EALWKKNDPK LRIFTQWGRV KNTQTKKRQQ MFQSSAYGRR ENTETTIEGR VIFDMLPYMQ
     RNHKLSSYSL NSVCAEYLSQ QKEDVHHSII SDLQNGTDAD RHRLAVYCLK DALLPQRLMD
     KLSVLINYVE MARVTGVPVS FLISRGQQIK VFSMILRKCR DVNLVVPTLR KSGAGADEGY
     EGATVLDPIK SYYEVPIATL DFASLYPSIM QAYNLCYSTM VSQHEASKID PSKYKRSENG
     HTFVHSNVKK GILPIILEEL LSARKRAKKD MKNAPNDFEK SVQNGRQLAL KVSANSVYGF
     TGAGVGQLPC VPIASSVTAY GRFLLEKTKA YVEEHYTVAH GYKHNAKVVY GDTDSVMVKF
     GTATVAETFP LAIEAAEKCS AIFPDPILLE FEKVYYPYLL MNKKRYAGLM WTQVEKYDYM
     DTKGLETVRR DNCALVREVI QTSLNKIIIE RNVPGAIDYV KSQIADLLQN KMDISRLVIT
     KSLGGKAEDY KVKQAHVELA ARMRKRDAGS APQMGDRVPY VIITAAKGAA NFEKAEDPIY
     VLENNIPIDC KWYLSNQLSK PLTRIFEPII DNVEKSLLQG DHTRKIFIPT PTAKKGSLMM
     FAHKKATCMG CKASINENEG NLCKYCLPKE VQIYLEKLAV LKEAELRYAH LWSEAQRIHG
     TLLTDIMCTG DGCACQFYRR KKVQNDIRLA QEQVDKFGR
//
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