ID B8ELU9_METSB Unreviewed; 526 AA.
AC B8ELU9;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE SubName: Full=FAD-binding domain protein {ECO:0000313|EMBL:ACK50730.1};
GN OrderedLocusNames=Msil_1784 {ECO:0000313|EMBL:ACK50730.1};
OS Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB
OS 13906 / BL2).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Beijerinckiaceae; Methylocella.
OX NCBI_TaxID=395965 {ECO:0000313|EMBL:ACK50730.1, ECO:0000313|Proteomes:UP000002257};
RN [1] {ECO:0000313|EMBL:ACK50730.1, ECO:0000313|Proteomes:UP000002257}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2
RC {ECO:0000313|Proteomes:UP000002257};
RX PubMed=20472789; DOI=10.1128/JB.00506-10;
RA Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B.,
RA Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.;
RT "Complete genome sequence of the aerobic facultative methanotroph
RT Methylocella silvestris BL2.";
RL J. Bacteriol. 192:3840-3841(2010).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001280; ACK50730.1; -; Genomic_DNA.
DR AlphaFoldDB; B8ELU9; -.
DR STRING; 395965.Msil_1784; -.
DR KEGG; msl:Msil_1784; -.
DR eggNOG; COG0369; Bacteria.
DR eggNOG; COG2878; Bacteria.
DR HOGENOM; CLU_001570_17_0_5; -.
DR OrthoDB; 9816402at2; -.
DR Proteomes; UP000002257; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd06199; SiR; 1.
DR Gene3D; 1.10.15.40; Electron transport complex subunit B, putative Fe-S cluster; 1.
DR Gene3D; 3.40.50.80; Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR007202; 4Fe-4S_dom.
DR InterPro; IPR003097; CysJ-like_FAD-binding.
DR InterPro; IPR017927; FAD-bd_FR_type.
DR InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR InterPro; IPR039261; FNR_nucleotide-bd.
DR InterPro; IPR023173; NADPH_Cyt_P450_Rdtase_alpha.
DR InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR19384:SF128; NADPH OXIDOREDUCTASE A; 1.
DR PANTHER; PTHR19384; NITRIC OXIDE SYNTHASE-RELATED; 1.
DR Pfam; PF00667; FAD_binding_1; 1.
DR Pfam; PF04060; FeS; 1.
DR Pfam; PF00175; NAD_binding_1; 1.
DR PRINTS; PR00371; FPNCR.
DR SUPFAM; SSF52343; Ferredoxin reductase-like, C-terminal NADP-linked domain; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
DR PROSITE; PS51656; 4FE4S; 1.
DR PROSITE; PS51384; FAD_FR; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00023192};
KW Cysteine biosynthesis {ECO:0000256|ARBA:ARBA00023192};
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000002257}.
FT DOMAIN 75..134
FT /note="4Fe-4S"
FT /evidence="ECO:0000259|PROSITE:PS51656"
FT DOMAIN 162..375
FT /note="FAD-binding FR-type"
FT /evidence="ECO:0000259|PROSITE:PS51384"
SQ SEQUENCE 526 AA; 57135 MW; F9B4710E0E5BC16A CRC64;
MSLHEAPPIS APLIPDNAPF SQEQRAWLNG FIAGLIAPDI AAAQGAPASP PAQAEDEITW
HDPSLPLAER MKLAEDKPLP LKLMAAMAQQ DCGQCGYDCQ KYAAAIASGE EKRLNLCAPG
EKPTARMVKS LVEGAGGEAA PAAAAAPAAL PKSSKPLGYA RENPVMARFL SRARLNKEDS
EKSTYHIEFD LSESGLDYTV GDSFGVFPAN DPELVDQILA AIRAPADFPI GEGTLREALI
HDLALHSAPD ALFELISYIV GGERRKLARS LAKGEDPEGD ASTLDVLAAL QKFPGIHPDP
EAFVESLEPL QPRLYSISSS PKVDPHRLSL TVDHVRYLIK DRVRRGVASS WLGERIQPGA
SFKAYIQRAH NFALPSSGET PIIMVGPGTG VAPFRAFLHE RCETKATGGA WLFFGHQRRA
ADFFYEEELT GFLANGALTK LSLAWSRDGE TKTYVQDKMR EEALSLWEWL EKGAHFYICG
DAKRMAADVE RAMMDIVAEA KGIDQKAAQA FVAELKKAGR YQADVY
//
