ID B8FM74_DESAL Unreviewed; 563 AA.
AC B8FM74;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:ACL05807.1};
GN OrderedLocusNames=Dalk_4123 {ECO:0000313|EMBL:ACL05807.1};
OS Desulfatibacillum aliphaticivorans.
OC Bacteria; Thermodesulfobacteriota; Desulfobacteria; Desulfobacterales;
OC Desulfatibacillaceae; Desulfatibacillum.
OX NCBI_TaxID=218208 {ECO:0000313|EMBL:ACL05807.1, ECO:0000313|Proteomes:UP000000739};
RN [1] {ECO:0000313|EMBL:ACL05807.1, ECO:0000313|Proteomes:UP000000739}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AK-01 {ECO:0000313|EMBL:ACL05807.1,
RC ECO:0000313|Proteomes:UP000000739};
RX PubMed=21651686; DOI=10.1111/j.1462-2920.2011.02516.x;
RA Callaghan A.V., Morris B.E., Pereira I.A., McInerney M.J., Austin R.N.,
RA Groves J.T., Kukor J.J., Suflita J.M., Young L.Y., Zylstra G.J., Wawrik B.;
RT "The genome sequence of Desulfatibacillum alkenivorans AK-01: a blueprint
RT for anaerobic alkane oxidation.";
RL Environ. Microbiol. 14:101-113(2012).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP001322; ACL05807.1; -; Genomic_DNA.
DR RefSeq; WP_015948854.1; NC_011768.1.
DR AlphaFoldDB; B8FM74; -.
DR KEGG; dal:Dalk_4123; -.
DR eggNOG; COG0457; Bacteria.
DR eggNOG; COG1032; Bacteria.
DR HOGENOM; CLU_021572_4_4_7; -.
DR Proteomes; UP000000739; Chromosome.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR001440; TPR_1.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF17; MG-PROTOPORPHYRIN IX MONOMETHYL ESTER OXIDATIVE CYCLASE 66KD SUBUNIT; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF00515; TPR_1; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SMART; SM00028; TPR; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
DR PROSITE; PS50005; TPR; 1.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000000739};
KW TPR repeat {ECO:0000256|ARBA:ARBA00022803, ECO:0000256|PROSITE-
KW ProRule:PRU00339}.
FT DOMAIN 8..143
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 177..402
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT REPEAT 481..514
FT /note="TPR"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00339"
SQ SEQUENCE 563 AA; 63421 MW; DEBFD627AB30B5CF CRC64;
MKVLLIYPPF LNKRVSSDDV ASIPIGLYHV AAVLRDAGHD AQVANWHMPN LDMAKAVQTI
ESFGPAVVGF SIVNGNRFGA LDMAAMLKER NPNLTIVLGG IGATFLWEHF LTHFPQVDYA
VVGEGEEAMA SLVKCLEIGD SRGIAEIPGV AARVDGAPVL TEQKGYIRDI DALPRPSRFY
KFQHLSLSRG CPHNCTFCGS PKFWGRTMRF HSADYFVRQL HTLAKSGEKF FYVSDDTFTL
KKDLVIRVCK EIIEAGLDIS WNAISRVDRV DEDILYWMRK AGCIQISYGV ESGCETTRDM
LNKNMSTQDI LRAFELTTRF GILSRAYFIY GCQGEGPEVL QANLDLMDAI KPLGAVFYIL
DIFPGTRLYE EYVQKNNLTD DIWLKRVEDI MYWETDPALT QDLIEETGDA LRNHFYRNLP
DYVYSLQLEN NPDLTMEHSD FYSRLGMTFT VGDYANDSRI SNASGVGEFL FEKALTFAQD
PRAYLGLGLL RQKCGQFEKA LDILEKALEL FPNDEQIRLC LGTNLMNLGA FGRALKHFEL
LPHLPGAIKY AEQCRACIAG SYR
//
