ID B8GDN7_METPE Unreviewed; 387 AA.
AC B8GDN7;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:ACL17388.1};
GN OrderedLocusNames=Mpal_2090 {ECO:0000313|EMBL:ACL17388.1};
OS Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanoregulaceae; Methanosphaerula.
OX NCBI_TaxID=521011 {ECO:0000313|EMBL:ACL17388.1, ECO:0000313|Proteomes:UP000002457};
RN [1] {ECO:0000313|EMBL:ACL17388.1, ECO:0000313|Proteomes:UP000002457}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1556 / DSM 19958 / E1-9c
RC {ECO:0000313|Proteomes:UP000002457};
RX PubMed=26543115; DOI=10.1128/genomea.01280-15;
RA Cadillo-Quiroz H., Browne P., Kyrpides N., Woyke T., Goodwin L., Detter C.,
RA Yavitt J.B., Zinder S.H.;
RT "Complete Genome Sequence of Methanosphaerula palustris E1-9CT, a
RT Hydrogenotrophic Methanogen Isolated from a Minerotrophic Fen Peatland.";
RL Genome Announc. 3:e01280-15(2015).
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. Anaerobic
CC sulfatase-maturating enzyme family. {ECO:0000256|ARBA:ARBA00023601}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001338; ACL17388.1; -; Genomic_DNA.
DR RefSeq; WP_012618707.1; NC_011832.1.
DR AlphaFoldDB; B8GDN7; -.
DR STRING; 521011.Mpal_2090; -.
DR GeneID; 7271567; -.
DR KEGG; mpl:Mpal_2090; -.
DR eggNOG; arCOG00945; Archaea.
DR HOGENOM; CLU_009273_10_0_2; -.
DR OrthoDB; 5620at2157; -.
DR Proteomes; UP000002457; Chromosome.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd21124; SPASM_CteB-like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR023885; 4Fe4S-binding_SPASM_dom.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR024018; CHP04083_rSAM.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR047602; SPASM_CteB-like.
DR InterPro; IPR023867; Sulphatase_maturase_rSAM.
DR NCBIfam; TIGR04085; rSAM_more_4Fe4S; 1.
DR NCBIfam; TIGR04083; rSAM_pep_methan; 1.
DR PANTHER; PTHR43273; ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED; 1.
DR PANTHER; PTHR43273:SF3; ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF13186; SPASM; 1.
DR SFLD; SFLDG01072; dehydrogenase_like; 1.
DR SFLD; SFLDG01384; thioether_bond_formation_requi; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000002457};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 1..213
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 387 AA; 43582 MW; AACFA49EB4FB55DF CRC64;
MKNPFHVMII PTLGCPSRCH YCWSSDETSP VMKIETVRDV VAWLNDFRDD RVTFTFHGGE
PLLAGADFYR QALPLLSEGL SHLNPEFAMQ TNLWLMTPEI AEILAAYHVP IGSSIDGPEE
INDSQRGRGY YEKTMKGYEI ARAHGLLVRF ICTFTNQSVR LREEIFDHFL QHGFTLKLHP
ALPSLKSDDP GQWALDPEEY GELLVYLLDR AIEKRDEIEV MNINDLCRCV FTRRGSVCTY
VDCMGNTFAV GPDGSIYPCY RFVGMPEYIL GHVSDHPTMD DLNGSPAGKR MFEYRDYVDT
ACGGCSHIQY CRGGCPYNAL AATPGEISGV DPHCVAYKRI FTEINDRLNR EMYESPMIGA
GSGSRSARRK EKPGVTALMR SIVSRSL
//
