ID B8GI85_METPE Unreviewed; 327 AA.
AC B8GI85;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE RecName: Full=DNA repair and recombination protein RadA {ECO:0000256|ARBA:ARBA00018144, ECO:0000256|HAMAP-Rule:MF_00348};
GN Name=radA {ECO:0000256|HAMAP-Rule:MF_00348};
GN OrderedLocusNames=Mpal_0040 {ECO:0000313|EMBL:ACL15436.1};
OS Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanoregulaceae; Methanosphaerula.
OX NCBI_TaxID=521011 {ECO:0000313|EMBL:ACL15436.1, ECO:0000313|Proteomes:UP000002457};
RN [1] {ECO:0000313|EMBL:ACL15436.1, ECO:0000313|Proteomes:UP000002457}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1556 / DSM 19958 / E1-9c
RC {ECO:0000313|Proteomes:UP000002457};
RX PubMed=26543115; DOI=10.1128/genomea.01280-15;
RA Cadillo-Quiroz H., Browne P., Kyrpides N., Woyke T., Goodwin L., Detter C.,
RA Yavitt J.B., Zinder S.H.;
RT "Complete Genome Sequence of Methanosphaerula palustris E1-9CT, a
RT Hydrogenotrophic Methanogen Isolated from a Minerotrophic Fen Peatland.";
RL Genome Announc. 3:e01280-15(2015).
CC -!- FUNCTION: Involved in DNA repair and in homologous recombination. Binds
CC and assemble on single-stranded DNA to form a nucleoprotein filament.
CC Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand
CC exchange between homologous DNA molecules. {ECO:0000256|HAMAP-
CC Rule:MF_00348, ECO:0000256|PIRNR:PIRNR005856}.
CC -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family.
CC {ECO:0000256|ARBA:ARBA00008050, ECO:0000256|HAMAP-Rule:MF_00348,
CC ECO:0000256|PIRNR:PIRNR005856}.
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DR EMBL; CP001338; ACL15436.1; -; Genomic_DNA.
DR RefSeq; WP_012616755.1; NC_011832.1.
DR AlphaFoldDB; B8GI85; -.
DR STRING; 521011.Mpal_0040; -.
DR GeneID; 7272209; -.
DR KEGG; mpl:Mpal_0040; -.
DR eggNOG; arCOG00415; Archaea.
DR HOGENOM; CLU_041732_0_0_2; -.
DR OrthoDB; 31129at2157; -.
DR Proteomes; UP000002457; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_00348; RadA_arch; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR011938; DNA_recomb/repair_RadA.
DR InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR NCBIfam; TIGR02236; recomb_radA; 1.
DR PANTHER; PTHR22942:SF39; DNA REPAIR PROTEIN RAD51 HOMOLOG 1; 1.
DR PANTHER; PTHR22942; RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF08423; Rad51; 1.
DR PIRSF; PIRSF005856; Rad51; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00278; HhH1; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF47794; Rad51 N-terminal domain-like; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00348};
KW DNA damage {ECO:0000256|HAMAP-Rule:MF_00348,
KW ECO:0000256|PIRNR:PIRNR005856};
KW DNA recombination {ECO:0000256|HAMAP-Rule:MF_00348,
KW ECO:0000256|PIRNR:PIRNR005856};
KW DNA-binding {ECO:0000256|HAMAP-Rule:MF_00348,
KW ECO:0000256|PIRNR:PIRNR005856};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00348}; Reference proteome {ECO:0000313|Proteomes:UP000002457}.
FT DOMAIN 80..262
FT /note="RecA family profile 1"
FT /evidence="ECO:0000259|PROSITE:PS50162"
FT DOMAIN 267..323
FT /note="RecA family profile 2"
FT /evidence="ECO:0000259|PROSITE:PS50163"
FT BINDING 109..116
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00348"
SQ SEQUENCE 327 AA; 35617 MW; C02DDEDB497C00B1 CRC64;
MAAGPLEIED LPGVGPTTAE KLRDAGFLTV ESIATASPTE LAEAAEIGEA SAKKIIKAAR
EIADIGGFKT GQDVFEQRKN VRKLKTFVPD FDELLGGGVE TQAITEVYGE FGSGKSQIVH
QMAVNAQLPE SVGGLNGSAI YIDTENTFRP ERIEQMVAGL DFPELELPSF EEFLNNIHVA
RAHTSDHQML LIDTARELAA ELKNSDHPVK IFIIDSLTAH FRAEYAGRGT LATRQQKLNR
HMHEFFKLID EHNAVGLVTN QVMSNPAVFF GDPTKPIGGN IVGHTATFRL YLRKSKGGKR
IARLVDSPNL PEGEAPFMVE EGGLKPC
//
