ID DNAA_CAUVN Reviewed; 490 AA.
AC B8GWW5; P35887;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE RecName: Full=Chromosomal replication initiator protein DnaA;
GN Name=dnaA; OrderedLocusNames=CCNA_00008;
OS Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=565050;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8288535; DOI=10.1128/jb.176.2.401-408.1994;
RA Zweiger G., Shapiro L.;
RT "Expression of Caulobacter dnaA as a function of the cell cycle.";
RL J. Bacteriol. 176:401-408(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NA1000 / CB15N;
RX PubMed=20472802; DOI=10.1128/jb.00255-10;
RA Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA Walunas T.L., Crosson S.;
RT "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL J. Bacteriol. 192:3678-3688(2010).
CC -!- FUNCTION: Plays an important role in the initiation and regulation of
CC chromosomal replication. Binds to the origin of replication; it binds
CC specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-
CC TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
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DR EMBL; U01667; AAA18927.1; -; Genomic_DNA.
DR EMBL; CP001340; ACL93475.1; -; Genomic_DNA.
DR PIR; B36947; B36947.
DR RefSeq; WP_010917898.1; NC_011916.1.
DR RefSeq; YP_002515383.1; NC_011916.1.
DR AlphaFoldDB; B8GWW5; -.
DR SMR; B8GWW5; -.
DR GeneID; 7333149; -.
DR KEGG; ccs:CCNA_00008; -.
DR PATRIC; fig|565050.3.peg.8; -.
DR HOGENOM; CLU_026910_3_0_5; -.
DR OrthoDB; 9807019at2; -.
DR PhylomeDB; B8GWW5; -.
DR Proteomes; UP000001364; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003688; F:DNA replication origin binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006270; P:DNA replication initiation; IEA:UniProtKB-UniRule.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR CDD; cd06571; Bac_DnaA_C; 1.
DR Gene3D; 1.10.1750.10; -; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.300.180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_00377; DnaA_bact; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001957; Chromosome_initiator_DnaA.
DR InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR013159; DnaA_C.
DR InterPro; IPR024633; DnaA_N_dom.
DR InterPro; IPR038454; DnaA_N_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010921; Trp_repressor/repl_initiator.
DR NCBIfam; TIGR00362; DnaA; 1.
DR PANTHER; PTHR30050; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR PANTHER; PTHR30050:SF2; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF08299; Bac_DnaA_C; 1.
DR Pfam; PF11638; DnaA_N; 1.
DR PRINTS; PR00051; DNAA.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00760; Bac_DnaA_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48295; TrpR-like; 1.
DR PROSITE; PS01008; DNAA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA replication; DNA-binding; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..490
FT /note="Chromosomal replication initiator protein DnaA"
FT /id="PRO_0000378284"
FT BINDING 189..196
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 334
FT /note="H -> L (in Ref. 1; AAA18927)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 490 AA; 53884 MW; 33BD9484340B5DC1 CRC64;
MTMKGGVASQ DFSAAIATAC EPAANVWSKV CVALKRELGD AAFGSWIAPA MLREAATGDV
VLVTSTGIAR DWIRRSAWRR IGELWAAHDA TGRRIDLKSR LEFEAAAGAY VEATPKAVAA
EPIEIVLPVS TDAPTVVAPS AKSPRTQGLQ ERFTFETFVP GPANEFAHAV ARRIANWADG
HFNPVLFHGP YGFGKTHLLN ALAWEAMRNA PEKRVVYLTA ERFLSTFVRA VMDRQTAAFK
EELRAADLLI IDDVHFIAGK QSTQEELFHT LTALVGEGGR VVFSADRPPS AMTEMDAHLR
SHLSAGLVCG LEPADRNLRL GILERKIQTL GAAHGFEPSI RPEVMQFLAD RFTDSVRELE
GALNTLSARA GEGLSRMTLD EVQAILRPHL RSGEKRITID DIQKATAEHY GMKQADLLSE
RRNRAVARPR QAAMWLAKQL TTRSLPDIGR RFGGRDHTTV LHAVRRIEAL RAEDSALSHD
LETLTRKLRG
//
