ID B8LEQ1_THAPS Unreviewed; 696 AA.
AC B8LEQ1;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN Name=RPOC {ECO:0000313|EMBL:EED86154.1};
GN ORFNames=THAPSDRAFT_bd997 {ECO:0000313|EMBL:EED86154.1};
OS Thalassiosira pseudonana (Marine diatom) (Cyclotella nana).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC Coscinodiscophyceae; Thalassiosirophycidae; Thalassiosirales;
OC Thalassiosiraceae; Thalassiosira.
OX NCBI_TaxID=35128 {ECO:0000313|EMBL:EED86154.1, ECO:0000313|Proteomes:UP000001449};
RN [1] {ECO:0000313|EMBL:EED86154.1, ECO:0000313|Proteomes:UP000001449}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1335 {ECO:0000313|EMBL:EED86154.1};
RX PubMed=15459382; DOI=10.1126/science.1101156;
RA Armbrust E.V., Berges J.A., Bowler C., Green B.R., Martinez D.,
RA Putnam N.H., Zhou S., Allen A.E., Apt K.E., Bechner M., Brzezinski M.A.,
RA Chaal B.K., Chiovitti A., Davis A.K., Demarest M.S., Detter J.C.,
RA Glavina T., Goodstein D., Hadi M.Z., Hellsten U., Hildebrand M.,
RA Jenkins B.D., Jurka J., Kapitonov V.V., Kroger N., Lau W.W., Lane T.W.,
RA Larimer F.W., Lippmeier J.C., Lucas S., Medina M., Montsant A., Obornik M.,
RA Parker M.S., Palenik B., Pazour G.J., Richardson P.M., Rynearson T.A.,
RA Saito M.A., Schwartz D.C., Thamatrakoln K., Valentin K., Vardi A.,
RA Wilkerson F.P., Rokhsar D.S.;
RT "The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and
RT metabolism.";
RL Science 306:79-86(2004).
RN [2] {ECO:0000313|EMBL:EED86154.1, ECO:0000313|Proteomes:UP000001449}
RP GENOME REANNOTATION.
RC STRAIN=CCMP1335 {ECO:0000313|EMBL:EED86154.1};
RG Diatom Consortium;
RA Grigoriev I., Grimwood J., Kuo A., Otillar R.P., Salamov A., Detter J.C.,
RA Schmutz J., Lindquist E., Shapiro H., Lucas S., Glavina del Rio T.,
RA Bruce D., Pitluck S., Rokhsar D., Armbrust V.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
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DR EMBL; DS999447; EED86154.1; -; Genomic_DNA.
DR RefSeq; XP_002297514.1; XM_002297478.1.
DR AlphaFoldDB; B8LEQ1; -.
DR STRING; 35128.B8LEQ1; -.
DR EnsemblProtists; EED86154; EED86154; THAPSDRAFT_bd997.
DR GeneID; 7447374; -.
DR KEGG; tps:THAPSDRAFT_bd997; -.
DR HOGENOM; CLU_030022_2_0_1; -.
DR InParanoid; B8LEQ1; -.
DR OMA; WGERTLP; -.
DR Proteomes; UP000001449; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR HAMAP; MF_01323; RNApol_bact_RpoC1; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR034678; RNApol_RpoC1.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF54; DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Reference proteome {ECO:0000313|Proteomes:UP000001449};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 332..593
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
SQ SEQUENCE 696 AA; 81256 MW; CF4907324B9C5FC7 CRC64;
MIRSEKEFDY IKIKLASPMR ILQWSHRKLP NGQFVGEVQK SETINYRTFK PEMDGLFCER
IFGPSKSLEC ACGKYKRVRY EELTESRVRR HRMGHINLIY PVTHVWYTNS RPNYIALLLE
VEQCEKRLDT GWTEFADLRD IKEKDNKDIP EYPSSEIECR NYLASPKAIL NCKEFLKEKS
KAKKSNIVNF YDERIKRIKL ASLAYFIAED EIAFYGLHWD LQQYRRCREL GFTGYPLKPH
SKSRNRRRNT PKYLLRSTPN YLIEKLNLDQ EIIKTRNFIM LCSKVLHKEQ PFYNFSHWSR
KWEYQRIYKL RDQSIKRIRI LENLLTTGAN PAWMIITILP VIPPALRPMI QLEGGRFATS
DLNELYRRII TRNNRLLRLL EIDAPQLIIL DTLIDNGKRG KIALSASNRP LKSLSDIIKG
KHGRFRQNLL GKRVDYSGRS VIVVGPSLKL NQCGLPYEMA IELFQPFIIP SNMKVAKNLL
QQNEPIINPV LEKVLANHPI FLNRAPTLHR LGIQAFQPII VQGRAIKLHP LVCSAFNADF
DGDQMAVHVP LSLEAQAECY MLMLAPYNFL SPANGEPIIM PSQDMVLGCY YLTVNNINGL
LGSNHYFADL NDVILAYNQD QIEIHTSIWV RYKHKISKPS NFIKKIILND KSYIEYYENI
QIRKDENDQI IVQYLQTTTG RVLLNYIIQT TLNLKP
//
