ID B8NT51_ASPFN Unreviewed; 604 AA.
AC B8NT51;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE SubName: Full=Acetolactate synthase, putative {ECO:0000313|EMBL:EED47279.1};
GN ORFNames=AFLA_053340 {ECO:0000313|EMBL:EED47279.1}, G4B84_000110
GN {ECO:0000313|EMBL:QMW24865.1};
OS Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS / JCM 12722 / SRRC 167).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=332952 {ECO:0000313|EMBL:EED47279.1, ECO:0000313|Proteomes:UP000001875};
RN [1] {ECO:0000313|EMBL:EED47279.1, ECO:0000313|Proteomes:UP000001875}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 /
RC SRRC 167 {ECO:0000313|Proteomes:UP000001875}, and NRRL3357
RC {ECO:0000313|EMBL:EED47279.1};
RX PubMed=25883274; DOI=10.1128/genomeA.00168-15;
RA Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT aflatoxin contamination of food and feed.";
RL Genome Announc. 3:E0016815-E0016815(2015).
RN [2] {ECO:0000313|EMBL:QMW24865.1, ECO:0000313|Proteomes:UP000515286}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL3357 {ECO:0000313|EMBL:QMW24865.1,
RC ECO:0000313|Proteomes:UP000515286};
RA Fountain J.C., Clevenger J.P., Nadon B., Youngblood R.C., Korani W.,
RA Chang P.-K., Starr D., Wang H., Isett B., Johnston H.R., Wiggins R.,
RA Chu Y., Agarwal G., Kemerait R.C., Pandey M.K., Bhatnagar D.,
RA Ozias-Akins P., Varshney R.K., Scheffler B.E., Vaughn J.N., Guo B.;
RT "Two New Chromosome-Level Aspergillus flavus Reference Genomes Reveal a
RT Large Insertion Potentially Contributing to Isolate Stress Tolerance and
RT Aflatoxin Production.";
RL Submitted (JUL-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the TPP enzyme family.
CC {ECO:0000256|ARBA:ARBA00007812, ECO:0000256|RuleBase:RU362132}.
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DR EMBL; EQ963483; EED47279.1; -; Genomic_DNA.
DR EMBL; CP059866; QMW24865.1; -; Genomic_DNA.
DR RefSeq; XP_002383459.1; XM_002383418.1.
DR AlphaFoldDB; B8NT51; -.
DR SMR; B8NT51; -.
DR STRING; 332952.B8NT51; -.
DR EnsemblFungi; EED47279; EED47279; AFLA_053340.
DR VEuPathDB; FungiDB:AFLA_011235; -.
DR eggNOG; KOG1185; Eukaryota.
DR HOGENOM; CLU_013748_4_0_1; -.
DR OMA; VPWIPRR; -.
DR OrthoDB; 1948175at2759; -.
DR Proteomes; UP000001875; Unassembled WGS sequence.
DR Proteomes; UP000515286; Chromosome 1.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0004737; F:pyruvate decarboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR CDD; cd02002; TPP_BFDC; 1.
DR CDD; cd07035; TPP_PYR_POX_like; 1.
DR Gene3D; 3.40.50.970; -; 2.
DR Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR InterPro; IPR045229; TPP_enz.
DR InterPro; IPR011766; TPP_enzyme_TPP-bd.
DR PANTHER; PTHR18968:SF164; PYRUVATE DECARBOXYLASE; 1.
DR PANTHER; PTHR18968; THIAMINE PYROPHOSPHATE ENZYMES; 1.
DR Pfam; PF02775; TPP_enzyme_C; 1.
DR Pfam; PF00205; TPP_enzyme_M; 1.
DR Pfam; PF02776; TPP_enzyme_N; 1.
DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
PE 3: Inferred from homology;
KW Thiamine pyrophosphate {ECO:0000256|RuleBase:RU362132}.
FT DOMAIN 12..142
FT /note="Thiamine pyrophosphate enzyme N-terminal TPP-
FT binding"
FT /evidence="ECO:0000259|Pfam:PF02776"
FT DOMAIN 220..327
FT /note="Thiamine pyrophosphate enzyme central"
FT /evidence="ECO:0000259|Pfam:PF00205"
FT DOMAIN 424..601
FT /note="Thiamine pyrophosphate enzyme TPP-binding"
FT /evidence="ECO:0000259|Pfam:PF02775"
SQ SEQUENCE 604 AA; 64906 MW; 36AA95E1C3044223 CRC64;
MADESTVGSP IVADAFLEAL AEAGVDYLFT VLGSDHPSII EAYIRRQNDP TRQYPKMILF
QHEFVAMSAA DGYARISHKP ACVIAHVDVG TAALGQGLHN ASSGRAPVVV FAGVAPSTLL
GEAPGSRSEH VQWYQDIRDQ AALVAPYSRF SAEIKSPHNV GSLVHRAVLM ATTGSPGPVY
LTATREILAT PIPSVEPRPK PVPSCHLGSL SPEAVEMIGN ALLEAKAPLV ITGYLGRSHR
AVQQLITLAD TVQGLRVFDS ELREVCFPAT HPACLTRSTG AAPAIQSADV ILVLDADVPW
IPRRVHPSPS AEIYHIDLDP RKERMNMFDI GASATFHADT TSALTQLNAY ITSSPRLPAL
QEAWTSRGQD LLTAHKEGKA RIDSRATAPL STPNEPCTVD YLCSRIRASV PQDTIYITDS
VTNQVPMTEQ LQLTRPGSHL TKGGSGLGWS GGAAIGASLA TARYDISDRP NLHLNETTKT
PFICNIIGDG SFVFSVPTAV YWAAHRYQTP FLTVILNNGG WNATRQCLAD VHPSGVAAGL
SNRDLGISLV EDGPDYGEVA KAAANGNLWT KRVRSVRELD VVLREGIRVV VEERKSVVLD
VVIR
//
