ID B8NUM5_ASPFN Unreviewed; 566 AA.
AC B8NUM5;
DT 03-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 03-MAR-2009, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=precorrin-2 dehydrogenase {ECO:0000256|ARBA:ARBA00012400};
DE EC=1.3.1.76 {ECO:0000256|ARBA:ARBA00012400};
GN ORFNames=AFLA_100780 {ECO:0000313|EMBL:EED46414.1};
OS Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS / JCM 12722 / SRRC 167).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=332952 {ECO:0000313|EMBL:EED46414.1, ECO:0000313|Proteomes:UP000001875};
RN [1] {ECO:0000313|EMBL:EED46414.1, ECO:0000313|Proteomes:UP000001875}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 /
RC SRRC 167 {ECO:0000313|Proteomes:UP000001875};
RX PubMed=25883274; DOI=10.1128/genomeA.00168-15;
RA Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT aflatoxin contamination of food and feed.";
RL Genome Announc. 3:E0016815-E0016815(2015).
CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC {ECO:0000256|RuleBase:RU003960}.
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DR EMBL; EQ963484; EED46414.1; -; Genomic_DNA.
DR RefSeq; XP_002383950.1; XM_002383909.1.
DR AlphaFoldDB; B8NUM5; -.
DR STRING; 332952.B8NUM5; -.
DR EnsemblFungi; EED46414; EED46414; AFLA_100780.
DR VEuPathDB; FungiDB:AFLA_010324; -.
DR eggNOG; KOG1527; Eukaryota.
DR HOGENOM; CLU_011276_2_2_1; -.
DR OMA; TPCAIVE; -.
DR Proteomes; UP000001875; Unassembled WGS sequence.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IEA:EnsemblFungi.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:EnsemblFungi.
DR GO; GO:0000103; P:sulfate assimilation; IEA:InterPro.
DR CDD; cd11642; SUMT; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR000878; 4pyrrol_Mease.
DR InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR InterPro; IPR006366; CobA/CysG_C.
DR InterPro; IPR012066; Met1_fungi.
DR InterPro; IPR028162; Met8_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR028281; Sirohaem_synthase_central.
DR InterPro; IPR003043; Uropor_MeTrfase_CS.
DR PANTHER; PTHR45790; SIROHEME SYNTHASE-RELATED; 1.
DR PANTHER; PTHR45790:SF6; UROPORPHYRINOGEN-III C-METHYLTRANSFERASE; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR Pfam; PF14823; Sirohm_synth_C; 1.
DR Pfam; PF14824; Sirohm_synth_M; 1.
DR Pfam; PF00590; TP_methylase; 1.
DR PIRSF; PIRSF036555; SUMT_yeast; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
DR SUPFAM; SSF53790; Tetrapyrrole methylase; 1.
DR PROSITE; PS00840; SUMT_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000256|RuleBase:RU003960}; NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003960}.
FT DOMAIN 150..176
FT /note="Siroheme synthase central"
FT /evidence="ECO:0000259|Pfam:PF14824"
FT DOMAIN 235..275
FT /note="Siroheme biosynthesis protein Met8 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14823"
FT DOMAIN 292..509
FT /note="Tetrapyrrole methylase"
FT /evidence="ECO:0000259|Pfam:PF00590"
SQ SEQUENCE 566 AA; 61792 MW; 54ACA813EFE5596E CRC64;
MKDASCDNGS PAPLLTAVYA ESQVHLIIGG NPLAAARCAK SLEAGAKPVI IAPDTGDLHF
SLSERIENGS VQWVRREFQD DDLKTLGREE VDHVVDTVFV TLGGNHPLRI LSNYLIGAHI
SKLCRRLRIP VNVSDAPELC TFSLLSTYSD GPLHIGITTS GRGCKLASRL RREISSSLPS
NLGTAIDRLG AVRRRLWAED YAAGLCTAPL EGDEDDITGQ SHTFNKLVTE DDISAAKTRR
IRWLSQICEY WPLQKLAAIS DADIDAILQA YSSGKNSLDS TNGLGHLEKK GKIVLAGSGP
GHPDMLTRAT YNAIQNADII LADKLVPEPV LKLIPRRTEV HIARKFPGNA DQAQEEFLQM
GLDSLRRGRY VLRLKQGDPY LYGRGGEEFE FFRGEGYTPV VLPGITSALS ASLFAEIPAT
HRGVSDQVLI CTGTGRKGAA PEPPTYVPTQ TVVFLMALHR LSALVESLTT PPQEGSRPRT
PWPKDTPCAI IERASCTDQR VIRSTLENVC LAFEAEGSRP PGLLVVGASC HILHPRKDEK
WTVEEGFRGL DDLRGEIVPE NDQKHD
//
