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Database: UniProt
Entry: B9DIT7
LinkDB: B9DIT7
Original site: B9DIT7 
ID   ADDB_STACT              Reviewed;        1155 AA.
AC   B9DIT7;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   18-JUL-2018, entry version 65.
DE   RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01452};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01452};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01452};
DE   AltName: Full=ATP-dependent helicase/nuclease AddB {ECO:0000255|HAMAP-Rule:MF_01452};
GN   Name=addB {ECO:0000255|HAMAP-Rule:MF_01452};
GN   OrderedLocusNames=Sca_0574;
OS   Staphylococcus carnosus (strain TM300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=396513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TM300;
RX   PubMed=19060169; DOI=10.1128/AEM.01982-08;
RA   Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G.,
RA   Schuster S.C., Goetz F.;
RT   "Genome analysis of the meat starter culture bacterium Staphylococcus
RT   carnosus TM300.";
RL   Appl. Environ. Microbiol. 75:811-822(2009).
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA
CC       helicase and an ATP-dependent, dual-direction single-stranded
CC       exonuclease. Recognizes the chi site generating a DNA molecule
CC       suitable for the initiation of homologous recombination. The AddB
CC       nuclease domain is not required for chi fragment generation; this
CC       subunit has 5' -> 3' nuclease activity. {ECO:0000255|HAMAP-
CC       Rule:MF_01452}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_01452}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01452};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB. {ECO:0000255|HAMAP-
CC       Rule:MF_01452}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 1
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01452}.
DR   EMBL; AM295250; CAL27488.1; -; Genomic_DNA.
DR   SMR; B9DIT7; -.
DR   STRING; 396513.Sca_0574; -.
DR   KEGG; sca:SCA_0574; -.
DR   eggNOG; ENOG4105C5N; Bacteria.
DR   eggNOG; COG3857; LUCA.
DR   HOGENOM; HOG000033809; -.
DR   KO; K16899; -.
DR   OMA; HFASHGL; -.
DR   Proteomes; UP000000444; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:InterPro.
DR   HAMAP; MF_01452; AddB_type1; 1.
DR   InterPro; IPR014140; DNA_helicase_suAddB.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02773; addB_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; ATP-binding; Complete proteome; DNA damage; DNA repair;
KW   Exonuclease; Hydrolase; Iron; Iron-sulfur; Metal-binding; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN         1   1155       ATP-dependent helicase/deoxyribonuclease
FT                                subunit B.
FT                                /FTId=PRO_0000379218.
FT   DOMAIN        1    278       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01452}.
FT   DOMAIN      270    584       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|HAMAP-Rule:MF_01452}.
FT   NP_BIND       9     16       ATP. {ECO:0000255|HAMAP-Rule:MF_01452}.
FT   METAL       785    785       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_01452}.
FT   METAL      1112   1112       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_01452}.
FT   METAL      1115   1115       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_01452}.
FT   METAL      1121   1121       Iron-sulfur (4Fe-4S). {ECO:0000255|HAMAP-
FT                                Rule:MF_01452}.
SQ   SEQUENCE   1155 AA;  134146 MW;  45F941D699C28341 CRC64;
     MSQLNAYIGR AGTGKSHAML DEIKTKMKQD PLGDPIIIIA PTQSTFQLEQ DFVKDPELNG
     SLRTEVLHFE RLSHRIFQEI GGLTEEYASK GALEMMIFDI LQSHRSELNL YQSQTKYYGF
     SAKLSEQIQD FKKYAVSPEQ LEQFISENPL QTRTQDKLHD IALVYRHLEE RLADNFVSSE
     DTLYKFIEKM DESKWLKRAE IYIDGFHNFS TLEYQIIEKL AQCAKSVSIL LTTNGDKDPF
     SLFRKTSSTL THIEEIAQRQ NIDFNLRRFT KQERFENRDL SHLEHSFNEV FFDKAAAEGN
     INILETSNVR EEVNAIARDI IRKAREENIR FQDVAVLYRD ETYAHLMESV FPEFDIPFNI
     DTKKSMTHHP VMEMIRSLLE VIESKWSFEP LMRLFKTQVL TKKFKDNRYL TDILENYVLE
     RGIYGQRWLD DKYFKIEQFN LMGLKRQPMT EETEADYQRV IDLKNYVIDK ILRFEKALAE
     ADTAETYAAA FYEAFEQFNL PSQLMTERDE LDLAGEHQQA EELDQVWNGF IQTLDDLATV
     FGNREMTQKR FLELFDVGLE QLEFVMIPQT LDQVSIGSMD LAKVDNKKHI YMLGMNDGTM
     PQAISNSGLI SDDEKKYFQE ETQLELSPTA DVLQMDEAFV CYIAMTRAST HVTFSYSLMG
     LNNDDKEVSP FIQNIRDLYT NLDVLNVQYA AQHNPLTVME HPHQTKIALF EELQSWLNHE
     LTAETWLEAY QAMLHNERLS RGLQYLTSAL TFDNKTIQLN QSLSKALYGD KINASVSRFE
     GYQQCPFKHY TSHGLRLNER TKYKLENFDL GDIFHSVLKY IADKIHGDFR NLTDASIRKL
     TQEALENILP EVQYNLLNSS AYYRYMSVRI GAIVQSTLTA LKYQGTFSKF RPQAFEKSFR
     KNPKSNEQLA AESLYTSQGI SINIRGQIDR IDTFNSKDRS FVNIIDYKSS GYSGTLDLTK
     VYYGLQMQMM TYMDVVLQNK ERLNLAETTE PGGLLYFHVH EPRVNFANWA EMDEDKRQEE
     LLKSFKLNGL INSDPEVLDA EDTRLEPKFK SDIVPIDVGA KGNLNKSSKV ADSQTIYKFI
     EHNKNNFIQI ASDIMDGHTQ VAPMKYKQKL PCEYCNYRSV CHVDGMIDSK KYRTVDESIN
     PIDLLNQESD EDDEE
//
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