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Database: UniProt
Entry: B9DRV0
LinkDB: B9DRV0
Original site: B9DRV0 
ID   ADDA_STRU0              Reviewed;        1220 AA.
AC   B9DRV0;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   25-APR-2018, entry version 62.
DE   RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE   AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   Name=addA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   OrderedLocusNames=SUB0686;
OS   Streptococcus uberis (strain ATCC BAA-854 / 0140J).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=218495;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-854 / 0140J;
RX   PubMed=19175920; DOI=10.1186/1471-2164-10-54;
RA   Ward P.N., Holden M.T.G., Leigh J.A., Lennard N., Bignell A.,
RA   Barron A., Clark L., Quail M.A., Woodward J., Barrell B.G., Egan S.A.,
RA   Field T.R., Maskell D., Kehoe M., Dowson C.G., Chanter N.,
RA   Whatmore A.M., Bentley S.D., Parkhill J.;
RT   "Evidence for niche adaptation in the genome of the bovine pathogen
RT   Streptococcus uberis.";
RL   BMC Genomics 10:54-54(2009).
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA
CC       helicase and an ATP-dependent, dual-direction single-stranded
CC       exonuclease. Recognizes the chi site generating a DNA molecule
CC       suitable for the initiation of homologous recombination. The AddA
CC       nuclease domain is required for chi fragment generation; this
CC       subunit has the helicase and 3' -> 5' nuclease activities.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC       Rule:MF_01451}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
DR   EMBL; AM946015; CAR41583.1; -; Genomic_DNA.
DR   RefSeq; WP_012658204.1; NC_012004.1.
DR   STRING; 218495.SUB0686; -.
DR   EnsemblBacteria; CAR41583; CAR41583; SUB0686.
DR   GeneID; 24161550; -.
DR   KEGG; sub:SUB0686; -.
DR   eggNOG; ENOG4107R2I; Bacteria.
DR   eggNOG; COG1074; LUCA.
DR   HOGENOM; HOG000285114; -.
DR   KO; K16898; -.
DR   OMA; KQSIYRW; -.
DR   OrthoDB; POG091H004Y; -.
DR   BioCyc; SUBE218495:G1G1Y-707-MONOMER; -.
DR   Proteomes; UP000000449; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006302; P:double-strand break repair; IEA:InterPro.
DR   Gene3D; 3.90.320.10; -; 1.
DR   HAMAP; MF_01451; AddA; 1.
DR   InterPro; IPR014152; DNA_helicase_suAddA.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR011604; Exonuc_phg/RecB_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   PANTHER; PTHR11070:SF14; PTHR11070:SF14; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 3.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding;
KW   Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN         1   1220       ATP-dependent helicase/nuclease subunit
FT                                A.
FT                                /FTId=PRO_0000379357.
FT   DOMAIN       26    482       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   DOMAIN      519    807       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   NP_BIND      47     54       ATP. {ECO:0000255|HAMAP-Rule:MF_01451}.
SQ   SEQUENCE   1220 AA;  140899 MW;  D955BD07E39CCA45 CRC64;
     MRFEPFLNQE EIRQLQEFEK NGSKAQKRTP EQIEAIYTNG QNILVSASAG SGKTFVMVER
     IIDKVLRGIS IENMFISTFT VKAANELKER LEKKLREGIL MTDDPEMKAY LNDQMQAIAL
     ADIGTMDAFT QKLLNEHGYL LGIPPKFRIM QDKSEQELLK NEIYKSLFEN YMASDHSQVF
     LRLVKNFSGN RKDSKSFRDQ VYKIYQFSQS TENPKQWLEN NFLLAANQYQ DYDDLPDETI
     DALLQSMKET AISLRDLTDL EEYGQMTKAG KPTAKYVKHL TMIEALNDWS MHFDSYYGKS
     QIGKLAQDLS ALLPSGTDVT VAGQKYPIFK DIQSKISQFR HLATLLTYQP ETLPLLLELQ
     SFVIDFTEAY LEAKKSENAF EFTDITHFAI EILQNEEAIR KVYQSKYHEV MVDEYQDNNH
     MQECLLELLS NGHNRFMVGD IKQSIYRFRQ ADPQIFNQKF KDYQENPENG KLILLKENFR
     SQSEVIDATN AVFSHLMDEA VGDILYDDNH YLKAGSDKQK IRYPENNCQV LIYDTDQKDN
     GEDEHDKEDE RRTEGQETLS PGEVNILVKE IIRLHQEEAV PFSDITLLVS SRTRNDLLFK
     TFNQYDIPLV SDGGQENYLK SVEVMVMLDT LRSINNPLND YALVALMRSP MFAFNEDQLA
     RIALQDSQEG KVEHFYEKVL NSLSHQGQHA ELITQELHDQ LELFDQSLNA WRDYSRTHSL
     YELIWKIYND RFYFDFVGLS PKAEQAQANL YALALRANQF EKTTFKGLTR FISMIDNILN
     TENDLADVDL AKPKEAVNVM TIHKSKGLEF KYVFILNCDK KFSMADIHSP LILSRQKGVG
     IKFLADVKKE LNATQLPSVK LYLETLPYQI NKKELKIATL SEQMRLLYVA MTRAEKKLYL
     IGKGSQEKLS QKFDTKREGQ HLPKALRESI TNFQDWLLAI HQVFDSKDLS FTIDFVTDNE
     LGPESIGSIS HQSNIMIDDL RDNRQSDSIA RAIDMLNNVN QLNQKYEAAI NLPTVRTPSQ
     LKKFYQPIMD IDGVEILADK SQTPTTFELP QFSKAKQVTS SQVGSALHEL MQRIKITSHV
     TEKDIQQASQ LVDAEAEVMA KIDLHKVKSF FEATELGRLI QVHHDKLYRE APFAMLKTDP
     QSQEKFVIRG IVDGFLLLED KIILFDYKTD HYKTPLEMKL KYQEQMALYA ESLRKAYDIS
     TIESYLILMG KERIEIVECS
//
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