ID B9QPR1_TOXGV Unreviewed; 653 AA.
AC B9QPR1; A0A0F7UU00;
DT 24-MAR-2009, integrated into UniProtKB/TrEMBL.
DT 24-MAR-2009, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE SubName: Full=Dihydrouridine synthase (Dus) protein {ECO:0000313|EMBL:ESS33365.1};
DE SubName: Full=Dihydrouridine synthase domain-containing protein {ECO:0000313|EMBL:CEL73531.1};
GN ORFNames=BN1205_080170 {ECO:0000313|EMBL:CEL73531.1}, TGVEG_280780
GN {ECO:0000313|EMBL:ESS33365.1};
OS Toxoplasma gondii (strain ATCC 50861 / VEG).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Toxoplasma.
OX NCBI_TaxID=432359 {ECO:0000313|EMBL:ESS33365.1, ECO:0000313|Proteomes:UP000002226};
RN [1] {ECO:0000313|EMBL:ESS33365.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=VEG {ECO:0000313|EMBL:ESS33365.1};
RA Paulsen I.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000002226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50861 / VEG {ECO:0000313|Proteomes:UP000002226};
RA Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E.;
RT "Annotation of Toxoplasma gondii VEG.";
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ESS33365.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=VEG {ECO:0000313|EMBL:ESS33365.1};
RA Sibley D., Venepally P., Karamycheva S., Hadjithomas M., Khan A., Brunk B.,
RA Roos D., Caler E., Lorenzi H.;
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:CEL73531.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=VEG {ECO:0000313|EMBL:CEL73531.1};
RX PubMed=25875305; DOI=10.1371/journal.pone.0124473;
RA Ramaprasad A., Mourier T., Naeem R., Malas T.B., Moussa E., Panigrahi A.,
RA Vermont S.J., Otto T.D., Wastling J., Pain A.;
RT "Comprehensive Evaluation of Toxoplasma gondii VEG and Neospora caninum LIV
RT Genomes with Tachyzoite Stage Transcriptome and Proteome Defines Novel
RT Transcript Features.";
RL PLoS ONE 10:e0124473-e0124473(2015).
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
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DR EMBL; LN714496; CEL73531.1; -; Genomic_DNA.
DR EMBL; AAYL02000106; ESS33365.1; -; Genomic_DNA.
DR AlphaFoldDB; B9QPR1; -.
DR STRING; 432359.B9QPR1; -.
DR PaxDb; 5811-TGME49_080780; -.
DR EnsemblProtists; ESS33365; ESS33365; TGVEG_280780.
DR VEuPathDB; ToxoDB:TGVEG_280780; -.
DR eggNOG; KOG2334; Eukaryota.
DR OMA; ANTLNYH; -.
DR Proteomes; UP000002226; Partially assembled WGS sequence.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:InterPro.
DR CDD; cd02801; DUS_like_FMN; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR035587; DUS-like_FMN-bd.
DR InterPro; IPR018517; tRNA_hU_synthase_CS.
DR PANTHER; PTHR45936; TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE; 1.
DR PANTHER; PTHR45936:SF1; TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE; 1.
DR Pfam; PF01207; Dus; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
DR PROSITE; PS01136; UPF0034; 1.
PE 4: Predicted;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000002226};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 257..497
FT /note="DUS-like FMN-binding"
FT /evidence="ECO:0000259|Pfam:PF01207"
FT REGION 1..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 127..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 520..546
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..91
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..113
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 653 AA; 70219 MW; 815EE442BD6700D0 CRC64;
MPGIAEGEQP LQETARNWDE ACGHGLPRQL DTVEENLNSR KRRKVDGDRM CDADSEGTGL
APVSSRQTEC VDEKEDGDDS EERKVLGRRP RTTVERPSAL SPSRGSSEDS ASAAPLFSCL
SPDCAASSLP ASAPAPSSAS ASSSMSETSS PASPPSSSSD SLLSRTHVCA SSDLSLPSRS
FLSASSSVAA GSVSDALPAR LAPPLSSCPS GSSSCLSSAS STGCRSSASD SVKLLEAEAS
LHFKPRGLAD RYCGKWILAP MVRIGILPFR LECLKYGADL VYTEETIDHK LLNSVRTFNE
DFSTIDFLHK SEKTCVFSTC LEERGKVVMQ LGTSDATRAL KAAMLVAQDV AAVDVNMGCP
KSFSINGGMG AALLKTPLIA TDILKTLRRN LDIPVTCKIR LLDTIHKTID FARLCENCGI
EAIAIHARET HERPSHRAHW DLFPALKSSL AIPLIANGDF LTRRDADVFQ EKIGADSLMF
ARGAMWDPSI FSWKKGTTKN VVAEFDSDSS AALSCEKETS SLYTSDPPPM GQNTDFSHPG
SSLPASESSR VKVMRGYIKR AVVCGAPYQA IKFCLQSMAA QCSSDRNVNL AITSSKSTRD
LCRALDLDLF YETVFPKLPP HANTLNYHKM RNLPEAASTE RLDSPLAFSS YLS
//
