GenomeNet

Database: UniProt
Entry: B9WZ64
LinkDB: B9WZ64
Original site: B9WZ64 
ID   ALR_PSETA               Reviewed;         357 AA.
AC   B9WZ64;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   14-APR-2009, sequence version 1.
DT   05-DEC-2018, entry version 41.
DE   RecName: Full=Alanine racemase {ECO:0000255|HAMAP-Rule:MF_01201};
DE            EC=5.1.1.1 {ECO:0000255|HAMAP-Rule:MF_01201};
GN   Name=alr;
OS   Pseudomonas taetrolens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=47884;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RX   PubMed=19300994; DOI=10.1007/s00253-009-1942-7;
RA   Matsui D., Oikawa T., Arakawa N., Osumi S., Lausberg F., Stabler N.,
RA   Freudl R., Eggeling L.;
RT   "A periplasmic, pyridoxal-5'-phosphate-dependent amino acid racemase
RT   in Pseudomonas taetrolens.";
RL   Appl. Microbiol. Biotechnol. 83:1045-1054(2009).
CC   -!- FUNCTION: Catalyzes the interconversion of L-alanine and D-
CC       alanine. Specific for alanine. {ECO:0000269|PubMed:19300994}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-alanine = D-alanine; Xref=Rhea:RHEA:20249,
CC         ChEBI:CHEBI:57416, ChEBI:CHEBI:57972; EC=5.1.1.1;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01201,
CC         ECO:0000269|PubMed:19300994};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01201};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.0. {ECO:0000269|PubMed:19300994};
CC       Temperature dependence:
CC         Optimum temperature is 35 degrees Celsius.
CC         {ECO:0000269|PubMed:19300994};
CC   -!- PATHWAY: Amino-acid biosynthesis; D-alanine biosynthesis; D-
CC       alanine from L-alanine: step 1/1. {ECO:0000255|HAMAP-
CC       Rule:MF_01201}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19300994}.
CC   -!- SIMILARITY: Belongs to the alanine racemase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01201}.
DR   EMBL; AB296103; BAH23434.1; -; Genomic_DNA.
DR   ProteinModelPortal; B9WZ64; -.
DR   SMR; B9WZ64; -.
DR   UniPathway; UPA00042; UER00497.
DR   GO; GO:0008784; F:alanine racemase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0030632; P:D-alanine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.40.37.10; -; 1.
DR   Gene3D; 3.20.20.10; -; 1.
DR   HAMAP; MF_01201; Ala_racemase; 1.
DR   InterPro; IPR000821; Ala_racemase.
DR   InterPro; IPR009006; Ala_racemase/Decarboxylase_C.
DR   InterPro; IPR011079; Ala_racemase_C.
DR   InterPro; IPR001608; Ala_racemase_N.
DR   InterPro; IPR020622; Ala_racemase_pyridoxalP-BS.
DR   InterPro; IPR029066; PLP-binding_barrel.
DR   Pfam; PF00842; Ala_racemase_C; 1.
DR   Pfam; PF01168; Ala_racemase_N; 1.
DR   PRINTS; PR00992; ALARACEMASE.
DR   SMART; SM01005; Ala_racemase_C; 1.
DR   SUPFAM; SSF50621; SSF50621; 1.
DR   SUPFAM; SSF51419; SSF51419; 1.
DR   TIGRFAMs; TIGR00492; alr; 1.
DR   PROSITE; PS00395; ALANINE_RACEMASE; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Pyridoxal phosphate.
FT   CHAIN         1    357       Alanine racemase.
FT                                /FTId=PRO_0000419112.
FT   ACT_SITE     33     33       Proton acceptor; specific for D-alanine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01201}.
FT   ACT_SITE    253    253       Proton acceptor; specific for L-alanine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01201}.
FT   BINDING     129    129       Substrate. {ECO:0000255|HAMAP-
FT                                Rule:MF_01201}.
FT   BINDING     301    301       Substrate; via amide nitrogen.
FT                                {ECO:0000255|HAMAP-Rule:MF_01201}.
FT   MOD_RES      33     33       N6-(pyridoxal phosphate)lysine.
FT                                {ECO:0000255|HAMAP-Rule:MF_01201}.
SQ   SEQUENCE   357 AA;  38814 MW;  9CD988FF18E944DA CRC64;
     MRPARALIDL QALRHNYRLA RELTGAKALA VIKADAYGHG AVRCALALEA EADGFAVACI
     EEALELRAAG IKAPVLLLEG FFEASELALI AEHDLWCVVH SLWQLEAIER TQLHKPLTVW
     LKLDSGMHRV GLHPKDYHEA YQRLLASGKV ARIVLMSHFA RADELDADAT AQQIAVFEAA
     RQGLAAECSL RNSPGVLGWP QAPSDWVRPG LMLYGATPFE VAQAEAERLQ PVMTLQSRVI
     SVRELPAGEP VGYGAKFVSP RPTRVGVVAM GYADGYPRQA PNGTPVLVAG KRTQLIGRVS
     MDMLSIDLTD VPEATVGSPV ELWGKHVLAS EVAAHAGTIP YQIFCNLKRV PRDYIGE
//
DBGET integrated database retrieval system