ID B9XMC7_PEDPL Unreviewed; 440 AA.
AC B9XMC7;
DT 14-APR-2009, integrated into UniProtKB/TrEMBL.
DT 14-APR-2009, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE SubName: Full=Polynucleotide adenylyltransferase/metal dependent phosphohydrolase {ECO:0000313|EMBL:EEF58969.1};
DE EC=2.7.7.72 {ECO:0000313|EMBL:EEF58969.1};
GN ORFNames=Cflav_PD2018 {ECO:0000313|EMBL:EEF58969.1};
OS Pedosphaera parvula (strain Ellin514).
OC Bacteria; Verrucomicrobiota; Verrucomicrobiae; Limisphaerales; Pedosphaera.
OX NCBI_TaxID=320771 {ECO:0000313|EMBL:EEF58969.1, ECO:0000313|Proteomes:UP000003688};
RN [1] {ECO:0000313|EMBL:EEF58969.1, ECO:0000313|Proteomes:UP000003688}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ellin514 {ECO:0000313|EMBL:EEF58969.1,
RC ECO:0000313|Proteomes:UP000003688};
RX PubMed=21460084; DOI=10.1128/JB.00299-11;
RA Kant R., van Passel M.W., Sangwan P., Palva A., Lucas S., Copeland A.,
RA Lapidus A., Glavina Del Rio T., Dalin E., Tice H., Bruce D., Goodwin L.,
RA Pitluck S., Chertkov O., Larimer F.W., Land M.L., Hauser L., Brettin T.S.,
RA Detter J.C., Han S., de Vos W.M., Janssen P.H., Smidt H.;
RT "Genome sequence of 'Pedosphaera parvula' Ellin514, an aerobic
RT Verrucomicrobial isolate from pasture soil.";
RL J. Bacteriol. 193:2900-2901(2011).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the tRNA nucleotidyltransferase/poly(A)
CC polymerase family. {ECO:0000256|RuleBase:RU003953}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEF58969.1}.
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DR EMBL; ABOX02000034; EEF58969.1; -; Genomic_DNA.
DR RefSeq; WP_007416966.1; NZ_ABOX02000034.1.
DR AlphaFoldDB; B9XMC7; -.
DR STRING; 320771.Cflav_PD2018; -.
DR OrthoDB; 9805698at2; -.
DR Proteomes; UP000003688; Unassembled WGS sequence.
DR GO; GO:0004810; F:CCA tRNA nucleotidyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0031123; P:RNA 3'-end processing; IEA:UniProt.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd00077; HDc; 1.
DR CDD; cd05398; NT_ClassII-CCAase; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 1.10.3090.10; cca-adding enzyme, domain 2; 1.
DR InterPro; IPR032810; CCA-adding_enz_C.
DR InterPro; IPR003607; HD/PDEase_dom.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002646; PolA_pol_head_dom.
DR InterPro; IPR032828; PolyA_RNA-bd.
DR PANTHER; PTHR46173; CCA TRNA NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL; 1.
DR PANTHER; PTHR46173:SF1; CCA TRNA NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL; 1.
DR Pfam; PF01743; PolyA_pol; 1.
DR Pfam; PF12627; PolyA_pol_RNAbd; 1.
DR Pfam; PF13735; tRNA_NucTran2_2; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81891; Poly A polymerase C-terminal region-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000313|EMBL:EEF58969.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:EEF58969.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000003688};
KW RNA-binding {ECO:0000256|RuleBase:RU003953};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003953}.
FT DOMAIN 21..141
FT /note="Poly A polymerase head"
FT /evidence="ECO:0000259|Pfam:PF01743"
FT DOMAIN 168..229
FT /note="tRNA nucleotidyltransferase/poly(A) polymerase RNA
FT and SrmB- binding"
FT /evidence="ECO:0000259|Pfam:PF12627"
FT DOMAIN 298..428
FT /note="CCA-adding enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13735"
SQ SEQUENCE 440 AA; 50306 MW; F339033367C8B813 CRC64;
MTLRETATEL VRRLQTAGFD AYWVGGCVRD YFLGREPKDF DVATNAVPEQ VERIFQRTIP
VGRKFGVLIV LEKGFEFQIA TFRAESDYQD GRHPEQVTFG DPKADASRRD FTINGLFYDP
VNDVLHDWVG GEVDLKAKVL RTIGEPAERF AEDHLRLLRA VRFAAQLNFQ IEAKTFAAMV
ANAPKIKTIS AERIREELMK VFRPPHAARG LDLLRESGLL EQVLPEIAAT ITCEQTPDYH
PEGSVYNHVR LMLSQLPEDA SPELPWAVLL HDVAKPVTAS RDPETGSIHF YGHEKVGAEM
AEVLLQRLKF PRKETDEIVA AVLYHMQFKD VPNMRKSTLR RMIMRETFPL ELALHRLDCL
GSHRMLDHYD FMVEQERELE KKPEILPPLL NGNDLMALGM QPGPAMGELL TELRDKQLQE
ELKTSDEARE WVLEKLKTKP
//
