GenomeNet

Database: UniProt
Entry: BETA_PSEPK
LinkDB: BETA_PSEPK
Original site: BETA_PSEPK 
ID   BETA_PSEPK              Reviewed;         565 AA.
AC   Q88CW6;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   08-MAY-2019, entry version 103.
DE   RecName: Full=Oxygen-dependent choline dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00750};
DE            Short=CDH {ECO:0000255|HAMAP-Rule:MF_00750};
DE            Short=CHD {ECO:0000255|HAMAP-Rule:MF_00750};
DE            EC=1.1.99.1 {ECO:0000255|HAMAP-Rule:MF_00750};
DE   AltName: Full=Betaine aldehyde dehydrogenase {ECO:0000255|HAMAP-Rule:MF_00750};
DE            Short=BADH {ECO:0000255|HAMAP-Rule:MF_00750};
DE            EC=1.2.1.8 {ECO:0000255|HAMAP-Rule:MF_00750};
GN   Name=betA {ECO:0000255|HAMAP-Rule:MF_00750};
GN   OrderedLocusNames=PP_5064;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 /
OS   KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M.,
RA   Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K.,
RA   Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D.,
RA   Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S.,
RA   Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B.,
RA   Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the
RT   metabolically versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- FUNCTION: Involved in the biosynthesis of the osmoprotectant
CC       glycine betaine. Catalyzes the oxidation of choline to betaine
CC       aldehyde and betaine aldehyde to glycine betaine at the same rate.
CC       {ECO:0000255|HAMAP-Rule:MF_00750}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=A + choline = AH2 + betaine aldehyde;
CC         Xref=Rhea:RHEA:17433, ChEBI:CHEBI:13193, ChEBI:CHEBI:15354,
CC         ChEBI:CHEBI:15710, ChEBI:CHEBI:17499; EC=1.1.99.1;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00750};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=betaine aldehyde + H2O + NAD(+) = betaine + 2 H(+) +
CC         NADH; Xref=Rhea:RHEA:15305, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15710, ChEBI:CHEBI:17750,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.2.1.8;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00750};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00750};
CC   -!- PATHWAY: Amine and polyamine biosynthesis; betaine biosynthesis
CC       via choline pathway; betaine aldehyde from choline (cytochrome c
CC       reductase route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00750}.
CC   -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00750}.
DR   EMBL; AE015451; AAN70629.1; -; Genomic_DNA.
DR   RefSeq; NP_747165.1; NC_002947.4.
DR   RefSeq; WP_010955611.1; NC_002947.4.
DR   SMR; Q88CW6; -.
DR   STRING; 160488.PP_5064; -.
DR   CAZy; AA3; Auxiliary Activities 3.
DR   PRIDE; Q88CW6; -.
DR   EnsemblBacteria; AAN70629; AAN70629; PP_5064.
DR   GeneID; 1043008; -.
DR   KEGG; ppu:PP_5064; -.
DR   PATRIC; fig|160488.4.peg.5406; -.
DR   eggNOG; ENOG4105CZ6; Bacteria.
DR   eggNOG; COG2303; LUCA.
DR   HOGENOM; HOG000139600; -.
DR   KO; K00108; -.
DR   OMA; LSWKIHM; -.
DR   BioCyc; PPUT160488:G1G01-5408-MONOMER; -.
DR   UniPathway; UPA00529; UER00385.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0008802; F:betaine-aldehyde dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008812; F:choline dehydrogenase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0019285; P:glycine betaine biosynthetic process from choline; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.50.50.60; -; 1.
DR   Gene3D; 4.10.450.10; -; 1.
DR   HAMAP; MF_00750; Choline_dehydrogen; 1.
DR   InterPro; IPR011533; BetA.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027424; Glucose_Oxidase_domain_2.
DR   InterPro; IPR012132; GMC_OxRdtase.
DR   InterPro; IPR000172; GMC_OxRdtase_N.
DR   InterPro; IPR007867; GMC_OxRtase_C.
DR   PANTHER; PTHR11552:SF157; PTHR11552:SF157; 1.
DR   Pfam; PF05199; GMC_oxred_C; 1.
DR   Pfam; PF00732; GMC_oxred_N; 1.
DR   PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01810; betA; 1.
DR   PROSITE; PS00623; GMC_OXRED_1; 1.
DR   PROSITE; PS00624; GMC_OXRED_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; FAD; Flavoprotein; NAD; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN         1    565       Oxygen-dependent choline dehydrogenase.
FT                                /FTId=PRO_0000205591.
FT   NP_BIND       6     35       FAD. {ECO:0000255|HAMAP-Rule:MF_00750}.
FT   ACT_SITE    475    475       Proton acceptor. {ECO:0000255|HAMAP-
FT                                Rule:MF_00750}.
SQ   SEQUENCE   565 AA;  62545 MW;  11C36EEBD7D6EF86 CRC64;
     MSQEFDYIIV GAGSAGNTLA TRLTEDAGVT VLLLEAGGPD YRFDFRTQMP AALAFPLQGR
     RYNWAYETDP EPYMDGRRME CGRGKGLGGS SLINGMCYIR GNAMDFDGWA ELPGLEDWTY
     LDCLPYFRKA ETRDIGPNDY HGGEGPVSVT TPKAGNNPLF HAMVEAGVQA GYPRTEDLNG
     YQQEGFGPMD RTVTKNGRRS STARGYLDQA KKRPNLTIVT HALTDRVLFD GKRAIGVTYL
     VGDSEERVEA RARKEVIVSS GAIASPQLLQ RSGVGPRALL ESLDIPVVHD LPGVGENLQD
     HLELYLQYAC TQPVSLYPSL LWWNQPAIGA EWMFNGTGIG ASNQFEAGGF IRTRPEFKWP
     NIQYHFLPVA INYNGSNGVK EHGFQAHMGS MRSPARGRIQ VKSKDPRQHP SILFNYMSTE
     QDWQEFRDGI RLTREIMAQP ALDPYRGREI SPGADVQTDE QLDKFIREHA ETAFHPSCSC
     KMGTDDMAVV DGEGRVHGMK GLRVVDASIM PLIITGNLNA TTIMIAEKIS DKIRGRKPLP
     RSTAKYYVAG DAPVKGKPMR EVKQG
//
DBGET integrated database retrieval system