GenomeNet

Database: UniProt
Entry: BGAL8_ORYSJ
LinkDB: BGAL8_ORYSJ
Original site: BGAL8_ORYSJ 
ID   BGAL8_ORYSJ             Reviewed;         673 AA.
AC   Q0DGD7; B7EF25; Q53WK7;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   07-NOV-2018, entry version 82.
DE   RecName: Full=Beta-galactosidase 8;
DE            Short=Lactase 8;
DE            EC=3.2.1.23;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os05g0539400, LOC_Os05g46200;
GN   ORFNames=OsJ_19375 {ECO:0000312|EMBL:EEE64524.1}, OSJNBa0052K01.15;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S.,
RA   Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
RA   Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
RA   Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
RA   Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
RA   Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
RA   Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
RA   Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
RA   Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
RA   Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
RA   Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA   Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA   Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA   Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
RA   Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of terminal non-reducing beta-D-
CC       galactose residues in beta-D-galactosides.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAV59405.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AC119291; AAV59405.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008211; BAF18086.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS95102.1; -; Genomic_DNA.
DR   EMBL; CM000142; EEE64524.1; -; Genomic_DNA.
DR   EMBL; AK068572; BAG90972.1; -; mRNA.
DR   RefSeq; XP_015639206.1; XM_015783720.1.
DR   UniGene; Os.14570; -.
DR   ProteinModelPortal; Q0DGD7; -.
DR   SMR; Q0DGD7; -.
DR   STRING; 39947.LOC_Os05g46200.1; -.
DR   CAZy; GH35; Glycoside Hydrolase Family 35.
DR   PaxDb; Q0DGD7; -.
DR   EnsemblPlants; Os05t0539400-01; Os05t0539400-01; Os05g0539400.
DR   GeneID; 4339457; -.
DR   Gramene; Os05t0539400-01; Os05t0539400-01; Os05g0539400.
DR   KEGG; osa:4339457; -.
DR   eggNOG; KOG0496; Eukaryota.
DR   eggNOG; COG1874; LUCA.
DR   HOGENOM; HOG000221607; -.
DR   InParanoid; Q0DGD7; -.
DR   KO; K12309; -.
DR   OMA; GWGKGIV; -.
DR   OrthoDB; EOG093603DJ; -.
DR   Reactome; R-OSA-1660662; Glycosphingolipid metabolism.
DR   Reactome; R-OSA-2022857; Keratan sulfate degradation.
DR   Reactome; R-OSA-2024096; HS-GAG degradation.
DR   Reactome; R-OSA-6798695; Neutrophil degranulation.
DR   Proteomes; UP000059680; Chromosome 5.
DR   ExpressionAtlas; Q0DGD7; differential.
DR   Genevisible; Q0DGD7; OS.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR   GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; -; 2.
DR   InterPro; IPR026283; B-gal_1-like.
DR   InterPro; IPR025300; BetaGal_jelly_roll_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421; PTHR23421; 1.
DR   Pfam; PF13364; BetaGal_dom4_5; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PIRSF; PIRSF006336; B-gal; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Complete proteome; Glycoprotein; Glycosidase; Hydrolase;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL        1     20       {ECO:0000255}.
FT   CHAIN        21    673       Beta-galactosidase 8.
FT                                /FTId=PRO_0000294160.
FT   ACT_SITE    189    189       Proton donor. {ECO:0000255}.
FT   ACT_SITE    272    272       Nucleophile. {ECO:0000255}.
FT   CARBOHYD     38     38       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    230    230       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    304    304       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    329    329       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    401    401       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    489    489       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    540    540       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
SQ   SEQUENCE   673 AA;  75674 MW;  6C323CDBBAB33AB6 CRC64;
     MGPSRSFQNL LLLLLPLALA LCSAAASGEA SRRFWVENDT FWKDGAPFQI VGGDVHYFRI
     VPEYWKDRLL RAKALGLNTI QTYVPWNLHE PKPLSWEFKG FTDIESYLRL AHELDMLVML
     RVGPYICGEW DLGGFPPWLL TIEPTIELRS SDSTYLSLVD RWWGVLLPKI APLLYSNGGP
     IIMVQIENEF GSFGDDKNYL HYLVEVARRY LGNDIMLYTT DGGAIGNLKN GTILQDDVFA
     AVDFDTGSNP WPIFQLQKEY NLPGKSAPLS SEFYTGWLTH WGERIATTDA SSTAKALKRI
     LCRNGSAVLY MAHGGTNFGF YNGANTGQNE SDYKADLTSY DYDAPIREYG DVHNAKYKAL
     RRVIHECTGI PLLQLPSKIE RASYGLVEVQ KVASLFDVIH NISDALKVAF SEQPLSMELM
     GQMFGFLLYT SEYQEKHSSS ILSIPKVHDR AQVFVSCSHG DVRKPRYVGI VERWSSKTLQ
     IPSLSCSSNV SLYILVENMG RVNYGPYIFD QKGILSSVEI DGIILRHWKM HPVSLNAVGN
     LSKLQLIMQM TDAEASKVSI YGDSENKLQD VSLYLNEGIS EEPAFYEGHF HIDSESEKKD
     TFISFRGWNK GVAFVNNFNI GRFWPAIGPQ CALYVPAPIL KPGDNVIVIF ELHSPNPELT
     IKLVKDPDFT CGQ
//
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