GenomeNet

Database: UniProt
Entry: BGALA_ASPNC
LinkDB: BGALA_ASPNC
Original site: BGALA_ASPNC 
ID   BGALA_ASPNC             Reviewed;        1007 AA.
AC   A2QAN3;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   16-JAN-2019, entry version 78.
DE   RecName: Full=Probable beta-galactosidase A;
DE            EC=3.2.1.23;
DE   AltName: Full=Lactase A;
DE   Flags: Precursor;
GN   Name=lacA; ORFNames=An01g12150;
OS   Aspergillus niger (strain CBS 513.88 / FGSC A1513).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
OX   NCBI_TaxID=425011;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 513.88 / FGSC A1513;
RX   PubMed=17259976; DOI=10.1038/nbt1282;
RA   Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G.,
RA   Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K.,
RA   Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M.,
RA   Breestraat S., Caddick M.X., Contreras R., Cornell M., Coutinho P.M.,
RA   Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M.,
RA   van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S.,
RA   Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T.,
RA   Henrissat B., Herweijer M., van den Hombergh J.P.T.W.,
RA   van den Hondel C.A.M.J.J., van der Heijden R.T.J.M.,
RA   van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P.,
RA   van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C.,
RA   Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G.,
RA   Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U.,
RA   Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J.,
RA   Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B.,
RA   Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.;
RT   "Genome sequencing and analysis of the versatile cell factory
RT   Aspergillus niger CBS 513.88.";
RL   Nat. Biotechnol. 25:221-231(2007).
CC   -!- FUNCTION: Cleaves beta-linked terminal galactosyl residues from
CC       gangliosides, glycoproteins, and glycosaminoglycans.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose
CC         residues in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000305}.
DR   EMBL; AM269982; CAK44114.1; -; Genomic_DNA.
DR   RefSeq; XP_001389622.1; XM_001389585.2.
DR   PDB; 5IFP; X-ray; 1.71 A; A=1-1007.
DR   PDB; 5IFT; X-ray; 2.45 A; A=1-1007.
DR   PDB; 5IHR; X-ray; 2.40 A; A=1-1007.
DR   PDB; 5JUV; X-ray; 2.27 A; A=1-1007.
DR   PDB; 5MGC; X-ray; 2.30 A; A=1-1007.
DR   PDB; 5MGD; X-ray; 2.15 A; A=1-1007.
DR   PDBsum; 5IFP; -.
DR   PDBsum; 5IFT; -.
DR   PDBsum; 5IHR; -.
DR   PDBsum; 5JUV; -.
DR   PDBsum; 5MGC; -.
DR   PDBsum; 5MGD; -.
DR   ProteinModelPortal; A2QAN3; -.
DR   SMR; A2QAN3; -.
DR   STRING; 5061.CADANGAP00001173; -.
DR   CAZy; GH35; Glycoside Hydrolase Family 35.
DR   PaxDb; A2QAN3; -.
DR   EnsemblFungi; CAK44114; CAK44114; An01g12150.
DR   GeneID; 4977988; -.
DR   KEGG; ang:ANI_1_1636014; -.
DR   HOGENOM; HOG000181922; -.
DR   Proteomes; UP000006706; Chromosome 2R.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004565; F:beta-galactosidase activity; IDA:AspGD.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IMP:AspGD.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.102.20.10; -; 1.
DR   Gene3D; 2.60.120.260; -; 2.
DR   Gene3D; 2.60.390.10; -; 1.
DR   InterPro; IPR018954; Betagal_dom2.
DR   InterPro; IPR037110; Betagal_dom2_sf.
DR   InterPro; IPR025972; BetaGal_dom3.
DR   InterPro; IPR036833; BetaGal_dom3_sf.
DR   InterPro; IPR025300; BetaGal_jelly_roll_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421; PTHR23421; 1.
DR   Pfam; PF10435; BetaGal_dom2; 1.
DR   Pfam; PF13363; BetaGal_dom3; 1.
DR   Pfam; PF13364; BetaGal_dom4_5; 2.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SMART; SM01029; BetaGal_dom2; 1.
DR   SUPFAM; SSF117100; SSF117100; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Carbohydrate metabolism; Complete proteome;
KW   Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL        1     18       {ECO:0000255}.
FT   CHAIN        19   1007       Probable beta-galactosidase A.
FT                                /FTId=PRO_5000219458.
FT   ACT_SITE    200    200       Proton donor. {ECO:0000255}.
FT   ACT_SITE    298    298       Nucleophile. {ECO:0000255}.
FT   BINDING      96     96       Substrate. {ECO:0000250}.
FT   BINDING     140    140       Substrate. {ECO:0000250}.
FT   BINDING     141    141       Substrate; via amide nitrogen.
FT                                {ECO:0000250}.
FT   BINDING     142    142       Substrate. {ECO:0000250}.
FT   BINDING     199    199       Substrate. {ECO:0000250}.
FT   BINDING     260    260       Substrate. {ECO:0000250}.
FT   BINDING     364    364       Substrate. {ECO:0000250}.
FT   CARBOHYD    156    156       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    373    373       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    402    402       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    422    422       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    478    478       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    522    522       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    622    622       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    739    739       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    760    760       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    777    777       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    805    805       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    914    914       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   DISULFID    205    206       {ECO:0000250}.
FT   DISULFID    266    315       {ECO:0000250}.
FT   STRAND       44     48       {ECO:0000244|PDB:5IFP}.
FT   STRAND       53     55       {ECO:0000244|PDB:5IFP}.
FT   STRAND       58     60       {ECO:0000244|PDB:5IFP}.
FT   STRAND       62     66       {ECO:0000244|PDB:5IFP}.
FT   HELIX        69     71       {ECO:0000244|PDB:5IFP}.
FT   HELIX        75     86       {ECO:0000244|PDB:5IFP}.
FT   TURN         87     89       {ECO:0000244|PDB:5IFP}.
FT   STRAND       92     96       {ECO:0000244|PDB:5IFP}.
FT   HELIX        99    102       {ECO:0000244|PDB:5IFP}.
FT   HELIX       113    115       {ECO:0000244|PDB:5IFP}.
FT   HELIX       118    127       {ECO:0000244|PDB:5IFP}.
FT   STRAND      130    137       {ECO:0000244|PDB:5IFP}.
FT   HELIX       144    147       {ECO:0000244|PDB:5IFP}.
FT   HELIX       150    154       {ECO:0000244|PDB:5IFP}.
FT   HELIX       164    183       {ECO:0000244|PDB:5IFP}.
FT   HELIX       186    188       {ECO:0000244|PDB:5IFP}.
FT   STRAND      190    199       {ECO:0000244|PDB:5IFP}.
FT   HELIX       213    225       {ECO:0000244|PDB:5IFP}.
FT   STRAND      236    239       {ECO:0000244|PDB:5IFP}.
FT   STRAND      257    259       {ECO:0000244|PDB:5IFP}.
FT   HELIX       281    288       {ECO:0000244|PDB:5IFP}.
FT   STRAND      295    302       {ECO:0000244|PDB:5IFP}.
FT   HELIX       312    318       {ECO:0000244|PDB:5IFP}.
FT   HELIX       321    332       {ECO:0000244|PDB:5IFP}.
FT   TURN        333    335       {ECO:0000244|PDB:5IFP}.
FT   STRAND      337    342       {ECO:0000244|PDB:5IFP}.
FT   HELIX       350    352       {ECO:0000244|PDB:5IFP}.
FT   HELIX       377    390       {ECO:0000244|PDB:5IFP}.
FT   HELIX       394    397       {ECO:0000244|PDB:5IFP}.
FT   STRAND      398    400       {ECO:0000244|PDB:5IFP}.
FT   STRAND      404    410       {ECO:0000244|PDB:5IFP}.
FT   STRAND      414    420       {ECO:0000244|PDB:5IFP}.
FT   STRAND      422    424       {ECO:0000244|PDB:5MGD}.
FT   STRAND      426    434       {ECO:0000244|PDB:5IFP}.
FT   STRAND      440    443       {ECO:0000244|PDB:5IFP}.
FT   STRAND      445    447       {ECO:0000244|PDB:5IFP}.
FT   STRAND      454    456       {ECO:0000244|PDB:5IFP}.
FT   STRAND      458    460       {ECO:0000244|PDB:5IFP}.
FT   STRAND      463    468       {ECO:0000244|PDB:5IFP}.
FT   STRAND      471    479       {ECO:0000244|PDB:5IFP}.
FT   STRAND      482    497       {ECO:0000244|PDB:5IFP}.
FT   STRAND      500    507       {ECO:0000244|PDB:5IFP}.
FT   STRAND      512    518       {ECO:0000244|PDB:5IFP}.
FT   STRAND      523    528       {ECO:0000244|PDB:5IFP}.
FT   STRAND      534    537       {ECO:0000244|PDB:5IFP}.
FT   STRAND      539    547       {ECO:0000244|PDB:5IFP}.
FT   STRAND      553    557       {ECO:0000244|PDB:5IFP}.
FT   STRAND      560    566       {ECO:0000244|PDB:5IFP}.
FT   HELIX       567    570       {ECO:0000244|PDB:5IFP}.
FT   STRAND      578    583       {ECO:0000244|PDB:5IFP}.
FT   HELIX       590    594       {ECO:0000244|PDB:5IFP}.
FT   STRAND      598    600       {ECO:0000244|PDB:5IFP}.
FT   STRAND      602    611       {ECO:0000244|PDB:5IFP}.
FT   STRAND      614    623       {ECO:0000244|PDB:5IFP}.
FT   STRAND      625    631       {ECO:0000244|PDB:5IFP}.
FT   STRAND      638    641       {ECO:0000244|PDB:5IFP}.
FT   STRAND      644    646       {ECO:0000244|PDB:5IFP}.
FT   STRAND      656    660       {ECO:0000244|PDB:5IFP}.
FT   HELIX       672    674       {ECO:0000244|PDB:5IFP}.
FT   STRAND      678    682       {ECO:0000244|PDB:5IFP}.
FT   HELIX       684    686       {ECO:0000244|PDB:5IFP}.
FT   STRAND      706    708       {ECO:0000244|PDB:5IFP}.
FT   STRAND      711    714       {ECO:0000244|PDB:5IFP}.
FT   HELIX       718    721       {ECO:0000244|PDB:5IFP}.
FT   STRAND      728    735       {ECO:0000244|PDB:5IFP}.
FT   STRAND      742    748       {ECO:0000244|PDB:5IFP}.
FT   STRAND      755    759       {ECO:0000244|PDB:5IFP}.
FT   STRAND      762    767       {ECO:0000244|PDB:5IFP}.
FT   STRAND      774    781       {ECO:0000244|PDB:5IFP}.
FT   STRAND      790    797       {ECO:0000244|PDB:5IFP}.
FT   HELIX       812    814       {ECO:0000244|PDB:5IFP}.
FT   STRAND      818    824       {ECO:0000244|PDB:5IFP}.
FT   HELIX       829    831       {ECO:0000244|PDB:5IFP}.
FT   STRAND      833    839       {ECO:0000244|PDB:5IFP}.
FT   TURN        840    843       {ECO:0000244|PDB:5IFP}.
FT   TURN        848    850       {ECO:0000244|PDB:5IFP}.
FT   STRAND      852    854       {ECO:0000244|PDB:5IFP}.
FT   HELIX       859    862       {ECO:0000244|PDB:5IFP}.
FT   TURN        863    866       {ECO:0000244|PDB:5IFP}.
FT   STRAND      867    869       {ECO:0000244|PDB:5IFP}.
FT   STRAND      875    877       {ECO:0000244|PDB:5IFP}.
FT   TURN        880    882       {ECO:0000244|PDB:5IFP}.
FT   STRAND      884    899       {ECO:0000244|PDB:5IFP}.
FT   STRAND      908    912       {ECO:0000244|PDB:5IFP}.
FT   STRAND      921    927       {ECO:0000244|PDB:5IFP}.
FT   STRAND      930    936       {ECO:0000244|PDB:5IFP}.
FT   TURN        937    939       {ECO:0000244|PDB:5IFP}.
FT   STRAND      944    947       {ECO:0000244|PDB:5IFP}.
FT   STRAND      955    966       {ECO:0000244|PDB:5IFP}.
FT   STRAND      977    981       {ECO:0000244|PDB:5IFP}.
SQ   SEQUENCE   1007 AA;  109712 MW;  CD1E91854D7A8A0A CRC64;
     MKLSSACAIA LLAAQAAGAS IKHRINGFTL TEHSDPAKRE LLQKYVTWDD KSLFINGERI
     MIFSGEFHPF RLPVKELQLD IFQKVKALGF NCVSFYVDWA LVEGKPGEYR ADGIFDLEPF
     FDAASEAGIY LLARPGPYIN AESSGGGFPG WLQRVNGTLR SSDKAYLDAT DNYVSHVAAT
     IAKYQITNGG PIILYQPENE YTSGCCGVEF PDPVYMQYVE DQARNAGVVI PLINNDASAS
     GNNAPGTGKG AVDIYGHDSY PLGFDCANPT VWPSGDLPTN FRTLHLEQSP TTPYAIVEFQ
     GGSYDPWGGP GFAACSELLN NEFERVFYKN DFSFQIAIMN LYMIFGGTNW GNLGYPNGYT
     SYDYGSAVTE SRNITREKYS ELKLLGNFAK VSPGYLTASP GNLTTSGYAD TTDLTVTPLL
     GNSTGSFFVV RHSDYSSEES TSYKLRLPTS AGSVTIPQLG GTLTLNGRDS KIHVTDYNVS
     GTNIIYSTAE VFTWKKFADG KVLVLYGGAG EHHELAISTK SNVTVIEGSE SGISSKQTSS
     SVVVGWDVST TRRIIQVGDL KILLLDRNSA YNYWVPQLAT DGTSPGFSTP EKVASSIIVK
     AGYLVRTAYL KGSGLYLTAD FNATTSVEVI GVPSTAKNLF INGDKTSHTV DKNGIWSATV
     DYNAPDISLP SLKDLDWKYV DTLPEIQSSY DDSLWPAADL KQTKNTLRSL TTPTSLYSSD
     YGFHTGYLLY RGHFTATGNE STFAIDTQGG SAFGSSVWLN GTYLGSWTGL YANSDYNATY
     NLPQLQAGKT YVITVVIDNM GLEENWTVGE DLMKTPRGIL NFLLAGRPSS AISWKLTGNL
     GGEDYEDKVR GPLNEGGLYA ERQGFHQPEP PSQNWKSSSP LEGLSEAGIG FYSASFDLDL
     PKGWDVPLFL NIGNSTTPSP YRVQVYVNGY QYAKYISNIG PQTSFPVPEG ILNYRGTNWL
     AVTLWALDSA GGKLESLELS YTTPVLTALG EVESVDQPKY KKRKGAY
//
DBGET integrated database retrieval system